The functional class evaluated in rheumatoid arthritis is associated with soluble TGF-β1 serum levels but not with G915C (Arg25Pro) TGF-β1 polymorphism

Abstract

The influence of genetic factors in rheumatoid arthritis (RA) has been described, including several cytokine genes such as transforming growth factor β (TGF-β) with regulatory effects on lymphocytes, dendritic cells, macrophages, chondrocytes, and osteoblasts, which are important in the RA pathogenesis. The G915C TGF-β1 polymorphism has been associated with soluble TGF-β1 (sTGF-β) serum levels. Thus, we studied the association of G915C (Arg25Pro) TGF-β1 polymorphism with sTGF-β1 serum levels in RA. We enrolled 120 RA patients and 120 control subjects (CS). The G915C TGF-β1 polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, and sTGF-β1 serum levels were quantified using an ELISA kit. The genotype frequency of G915C TGF-β1 polymorphism in RA and CS was G/G (91.7%), G/C (8.3%), C/C (0%) and G/G (85.8%), G/C (14.2%), C/C (0%), respectively, without significant differences. Moreover, the G/G TGF-β1 genotype carriers presented the highest disability index evaluated for the Spanish HAQ-DI score (P < 0.001). In addition, the sTGF-β1 serum levels were higher in RA (182.2 ng/mL) than CS (160.2 ng/mL), there was not significant difference. However, we found a positive correlation between the sTGF-β1 serum levels and the functional class (r = 0.472, P = 0.023). In conclusion, the G915C (Arg25Pro) TGF-β1 polymorphism is not associated with RA, but the sTGF-β1 serum levels are related with the functional class in RA.

Peripheral regulatory T cells and anti-inflammatory cytokines in children with juvenile idiopathic arthritis

“…In rheumatoid arthritis one publication reported elevated serum levels of IL-10, but this observation was not further confirmed by other researchers (Cush et al, 1995). In the only study analyzing serum level of TGF-β1 in patients with RA, this parameter did not differ significantly from that found in healthy individuals (Muñoz-Valle et al, 2012). Our hereby presented findings imply that JIA is not associated with a deficit in anti-inflammatory cytokines.…”

Retracted: Long non‐coding RNA XIST binding to let‐7c‐5p contributes to rheumatoid arthritis through its effects on proliferation and differentiation of osteoblasts via regulation of STAT3

“…As one of genetic factors, TGF‐β exerts regulatory effects on osteoblasts. In fact, a high level of TGF‐β expression has been correlated to a faster function recovery in RA 53 . Furthermore, IGF1 is regarded as a key mediator in the developmental processes, including cell proliferation and differentiation, and RA patients were associated with a low level of IGF1 expression 54 .…”

Genetic Case-Control Study for Eight Polymorphisms Associated with Rheumatoid Arthritis

“…The TGFβ1 protein has been found in the synovial fluid of RA patients [ 11 ]. The T869C polymorphism is associated with the soluble TGFβ1 serum levels [ 12 ].…”