The functions and unique features of long intergenic non-coding RNA

Ransohoff, Julia D.; Wei, Yuning; Khavari, Paul A.

doi:10.1038/nrm.2017.104

Cited by 1,068 publications

(856 citation statements)

References 167 publications

(252 reference statements)

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“…Accumulating evidence have demonstrated that the function of lncRNAs could be either as an oncogene, or a tumor suppressor gene, or both involved in tumor prognosis and progression, depending on the circumstance(s) [13–15]. Amongst them, many lncRNAs, such as MALAT1, H19, and HOTAIR have been demonstrated as a critical regulator of carcinogenesis and development of many cancers [16–18].…”

Section: Introductionmentioning

confidence: 99%

High LINC01605 expression predicts poor prognosis and promotes tumor progression via up-regulation of MMP9 in bladder cancer

et al. 2018

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The advent of high-throughput sequencing methods has facilitated identification of novel long non-coding RNAs (lncRNAs), which have been demonstrated to play an important role in multiple tumors. Moreover, with the assistance of bioinformatics analysis, LINC01605 has been found to be up-regulated in bladder cancer (BC) tissues compared with normal tissues. Hence, the present study was to explore its specific biological role and related mechanism in BC. The relative expression level of LINC01605 was measured in a cohort of BC tissues with matched normal tissues as well as human BC cell lines by quantitative real-time PCR (qRT-PCR). Survival analysis was performed to explore the relationship between LINC01605 expression and the prognosis of BC patients. The biological function of LINC01605 was studied in vitroand in vivo, by means of CCK-8 assay, colony formation assay, transwell assay, and tumor xenografts mice model. LINC01605 was found to be frequently highly expressed in both human BC cells and tissues. Survival analysis indicated that high LINC01605 expression was associated with higher histological grade and clinical stages. In addition, down-regulated LINC01605 in BC cells could significantly inhibit the abilities of proliferation, migration, and invasion in vitro and knockdown of LINC01605 in subcutaneous xenograft tumor model could impede tumorigenesis in vivo. Mechanistically, LINC01605 could activate epithelial–mesenchymal transition (EMT) signaling pathway and promote the expression of matrix metallopeptidase (MMP) 9 (MMP9). In summary, our results shed light on that LINC01605, as a new prognostic biomarker, could promote the proliferation, migration, and invasion of BC cells via activating EMT signaling pathway and up-regulating MMP9 expression.

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Section: Introductionmentioning

confidence: 99%

High LINC01605 expression predicts poor prognosis and promotes tumor progression via up-regulation of MMP9 in bladder cancer

et al. 2018

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“…Bioinformatic studies have annotated tens of thousands of lncRNAs—transcripts > 200 nt in length that do not appear to code for proteins—and such lncRNAs can be mapped to every chromosome . While certain lncRNAs are now known to play key roles in critical cellular processes —such as lncRNA XIST in X chromosome inactivation —the vast majority of lncRNAs have not been demonstrated to have significant biological functions. For this emerging field of research, an important next step is to identify which lncRNAs regulate important aspects of cell and molecular biology, and lncRNA loss‐of‐function and gain‐of‐function approaches are a mainstay for such discovery.…”

Section: Introductionmentioning

confidence: 99%

Modulating the expression of long non‐coding RNA s for functional studies

Liu

Lim

2018

EMBO Reports

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Long non‐coding RNAs (lncRNAs) have emerged as important regulators of cell biology. The mechanisms by which lncRNAs function are likely numerous, and most are poorly understood. Currently, the mechanisms of functional lncRNAs include those that directly involve the lncRNA transcript, the process of their own transcription and splicing, and even underlying transcriptional regulatory elements within the genomic DNA that encodes the lncRNA. As our understanding of lncRNA biology evolves, so have the methods that are utilized to elucidate their functions. In this review, we survey a collection of different methods used to modulate lncRNA expression levels for the assessment of biological function. From RNA‐targeted strategies, genetic deletions, to engineered gene regulatory systems, the advantages and caveats of each method will be discussed. Ultimately, the selection of tools will be guided by which potential lncRNA mechanisms are being investigated, and no single method alone will likely be sufficient to reveal the function of any particular lncRNA.

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“…Accumulating evidence suggests that lncRNAs play important roles in the different biological process such as cell growth and differentiation, apoptosis and immune responses. Indeed, deregulation of lncRNA has been associated with different human diseases, including cancer, tumorigenesis and neurological diseases . LncRNAs are predominantly found in the cytoplasm and nucleus suggesting that lncRNAs regulate cell functions in both locations.…”

Section: Introductionmentioning

confidence: 99%

“…LncRNAs are predominantly found in the cytoplasm and nucleus suggesting that lncRNAs regulate cell functions in both locations. Indeed, studies have shown that lncRNAs can control gene expression by epigenetic regulation and also influencing transcription factors . Several aspects of lncRNA can be exploited for therapeutic purposes because of two major advantages.…”

Section: Introductionmentioning

confidence: 99%

Long non‐coding RNA GAPLINC promotes angiogenesis by regulating miR‐211 under hypoxia in human umbilical vein endothelial cells

Qiu

et al. 2019

J Cellular Molecular Medi

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In this study, we investigated the role of a long non‐coding RNA GAPLINC in angiogenesis using human umbilical vein endothelial cells (HUVEC). We found that hypoxia and hypoxia‐inducible factor 1α (HIF‐1α) increased the expression of GAPLINC in HUVEC cells. Moreover, GAPLINC overexpression down‐regulated miR‐211 and up‐regulated Bcl2 protein expression. Further rescue experiments confirmed that hypoxia directly increased GAPLINC expression. GAPLINC overexpression also increased cell migration and vessel formation which promoted angiogenesis, and these changes were attributed to the increased expression of vascular endothelial growth factor receptors (VEGFR) and delta‐like canonical notch ligand 4 (DLL4) receptors. Finally, we demonstrated that GAPLINC promotes vessel formation and migration by regulating MAPK and NF‐kB signalling pathways. Taken together, these findings comprehensively demonstrate that overexpression of GAPLINC increases HUVEC cells angiogenesis under hypoxia condition suggesting that GAPLINC can be a potential target for critical limb ischaemia (CLI) treatment.

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The functions and unique features of long intergenic non-coding RNA

Cited by 1,068 publications

References 167 publications

High LINC01605 expression predicts poor prognosis and promotes tumor progression via up-regulation of MMP9 in bladder cancer

High LINC01605 expression predicts poor prognosis and promotes tumor progression via up-regulation of MMP9 in bladder cancer

Modulating the expression of long non‐coding RNA s for functional studies

Long non‐coding RNA GAPLINC promotes angiogenesis by regulating miR‐211 under hypoxia in human umbilical vein endothelial cells

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