The galactose‐binding lectin isolated from <i>Bauhinia bauhinioides</i> Mart seeds inhibits neutrophil rolling and adhesion via primary cytokines

Girão, Deysen Kerlla Fernandes Bezerra; Cavada, Benildo S.; Pires, Alana de Freitas; Martins, Timna Varela; Franco, Álvaro Xavier; Morais, Cecília Mendes; Nascimento, Kyria S.; Delatorre, P.; Silva, Helton Colares da; Nagano, Celso Shiniti; Assreuy, Ana Maria Sampaio; Soares, Pedro Marcos Gomes

doi:10.1002/jmr.2441

Cited by 15 publications

(6 citation statements)

References 44 publications

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“…A number of other studies also detail important lectin‐mediated binding interactions during PMN trafficking. For example, macrophage galactose type lectin 1 deficiency is associated with increased neutrophilia and hyperinflammation during gram negative pneumonia, while the Galactose‐binding Bauhinia bauhinioides lectin inhibits PMN peritoneal adhesion and migration in response to cytokines including TNF‐α In addition, it has been reported that other lectins including Concavalin A and peanut agglutinin lectin stimulate a chemotactic response in human neutrophils . However, these studies did not identify the specific neutrophil glycoproteins that are the binding targets for these lectins.…”

Section: Discussionmentioning

confidence: 99%

Regulation of neutrophil function by selective targeting of glycan epitopes expressed on the integrin CD11b/CD18

et al. 2019

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Polymorphonuclear neutrophils (PMNs) play a critical role in the innate immune response to invading pathogens. However, dysregulated mucosal trafficking of PMNs and associated epithelial tissue damage is a pathological hallmark of numerous inflammatory conditions including inflammatory bowel disease. The glycoprotein CD11b/CD18 plays a well‐described role in regulating PMN transepithelial migration and PMN inflammatory functions. Previous studies have demonstrated that targeting of the N‐linked glycan Lewis X on CD11b blocks PMN transepithelial migration (TEpM). Given evidence of glycosylation‐dependent regulation of CD11b/CD18 function, we performed MALDI TOF Mass Spectrometry (MS) analyses on CD11b/CD18 purified from human PMNs. Unusual glycan epitopes identified on CD11b/CD18 included high Mannose oligosaccharides recognized by the Galanthus Nivalis lectin and biantennary galactosylated N‐glycans recognized by the Phaseolus Vulgaris erythroagglutinin lectin. Importantly, we show that selective targeting of glycans on CD11b with such lectins results in altered intracellular signaling events that inhibit TEpM and differentially affect key PMN inflammatory functions including phagocytosis, superoxide release and apoptosis. Taken together, these data demonstrate that discrete glycan motifs expressed on CD11b/CD18 such as biantennary galactose could represent novel targets for selective manipulation of CD11b function and reduction of PMN‐associated tissue damage in chronic inflammatory diseases.

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Section: Discussionmentioning

confidence: 99%

Regulation of neutrophil function by selective targeting of glycan epitopes expressed on the integrin CD11b/CD18

et al. 2019

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“…A number of other studies also detail important lectin mediated binding interactions during PMN trafficking. For example, Macrophage galactose type lectin 1 deficiency is associated with increased neutrophilia and hyperinflammation during gram negative pneumonia (58), while the Galactose binding Bauhinia bauhinioides lectin inhibits PMN peritoneal adhesion and migration in response to cytokines including TNF-α (59). In addition, it has been reported that other lectins including Concavalin A and peanut agglutinin lectin stimulate a chemotactic response in human neutrophils (60).…”

Section: Discussionmentioning

confidence: 99%

Regulation of Neutrophil Function by Selective Targeting of Glycan Epitopes Expressed on the Integrin CD11b/CD18

et al. 2020

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Polymorphonuclear neutrophils (PMN) play a critical role in the innate immune response to invading pathogens. However, dysregulated mucosal trafficking of PMN and associated epithelial tissue damage is a pathological hallmark of numerous inflammatory conditions including inflammatory bowel disease and chronic obstructive pulmonary disease. The glycoprotein CD11b/CD18 plays a well described role in regulating PMN transepithelial migration and PMN inflammatory functions. Previous studies have demonstrated that targeting of the N‐linked glycan Lewis X on CD11b blocks PMN transepithelial migration (TEpM). Given evidence of glycosylation dependent regulation of CD11b/CD18 function, we performed MALDI TOF Mass Spectrometry (MS) analyses on CD11b/CD18 purified from human PMN. MS analyses revealed a surprising lack of sialylation on CD11b glycans. Unusual glycan epitopes identified on CD11b/CD18 included high Mannose oligosaccharides recognized by the Galanthus Nivalis (GNA) lectin and biantennary galactosylated N‐glycans recognized by the Phaseolus Vulgaris erythroagglutinin (PHA‐E) lectin. Importantly, we show that selective targeting of glycans on CD11b with such lectins results in altered intracellular signaling events that inhibit TEpM and differentially affect key PMN inflammatory functions including phagocytosis, superoxide release and apoptosis. Taken together, these data demonstrate that discrete glycan motifs expressed on CD11b/CD18 such as biantennary galactose could represent novel targets for selective manipulation of CD11b function and reduction of PMN associated tissue damage in chronic inflammatory diseases. Support or Funding Information This work was supported by the Crohn’s and colitis foundation (SRA # 544596)

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“…Recent research shows that lectin exhibits promising control over tumor cell metastasis by inducing programmed cell death. This potentially offers antiinflammatory properties via the lectin domain in legumes, and demonstrates immunomodulatory effects [234][235][236][237][238]. Additionally, these proteins demonstrate antifungal action and generation of cytokines in both in vitro and in vivo [239][240][241].…”

Section: Uses Of Antinutritional Factors In Research and Future Direc...mentioning

confidence: 99%

A Review of Bioactive Compound Effects from Primary Legume Protein Sources in Human and Animal Health

Shea,

Ogando do Granja,

Fletcher

et al. 2024

CIMB

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The global demand for sustainable and nutritious food sources has catalyzed interest in legumes, known for their rich repertoire of health-promoting compounds. This review delves into the diverse array of bioactive peptides, protein subunits, isoflavones, antinutritional factors, and saponins found in the primary legume protein sources—soybeans, peas, chickpeas, and mung beans. The current state of research on these compounds is critically evaluated, with an emphasis on the potential health benefits, ranging from antioxidant and anticancer properties to the management of chronic diseases such as diabetes and hypertension. The extensively studied soybean is highlighted and the relatively unexplored potential of other legumes is also included, pointing to a significant, underutilized resource for developing health-enhancing foods. The review advocates for future interdisciplinary research to further unravel the mechanisms of action of these bioactive compounds and to explore their synergistic effects. The ultimate goal is to leverage the full spectrum of benefits offered by legumes, not only to advance human health but also to contribute to the sustainability of food systems. By providing a comprehensive overview of the nutraceutical potential of legumes, this manuscript sets a foundation for future investigations aimed at optimizing the use of legumes in the global pursuit of health and nutritional security.

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The galactose‐binding lectin isolated from Bauhinia bauhinioides Mart seeds inhibits neutrophil rolling and adhesion via primary cytokines

Cited by 15 publications

References 44 publications

Regulation of neutrophil function by selective targeting of glycan epitopes expressed on the integrin CD11b/CD18

Regulation of neutrophil function by selective targeting of glycan epitopes expressed on the integrin CD11b/CD18

Regulation of Neutrophil Function by Selective Targeting of Glycan Epitopes Expressed on the Integrin CD11b/CD18

A Review of Bioactive Compound Effects from Primary Legume Protein Sources in Human and Animal Health

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