The Gcd10p/Gcd14p complex is the essential two-subunit tRNA(1-methyladenosine) methyltransferase of Saccharomyces cerevisiae

tRNAHis maturation: An essential yeast protein catalyzes addition of a guanine nucleotide to the 5′ end of tRNAHis

“…Gcd10p/Gcd14p catalyzes formation of m 1 A 58 in a number of tRNAs (Anderson et al 1998(Anderson et al , 2000, and is essential for modification of tRNA i Met (Calvo et al 1999), and Tad2p/Tad3p catalyzes formation of I 34 in the wobble position of the anticodon of tRNA Ala (Gerber and Keller 1999), and is presumably essential for proper decoding during translation. Strains lacking each of three other modification enzymes are viable, but have distinct growth defects (Lecointe et al 1998;Bjork et al 2001;Pintard et al 2002), whereas lack of most other modification proteins has only subtle growth defects.…”

Molecular Chaperones and Quality Control in Noncoding RNA Biogenesis

“…(B) Methylation of adenosine 58 is required for stable accumulation of tRNA i Met . In yeast carrying mutations in either TRM6 or TRM61, which encode the two subunits of the tRNA (m 1 A58) methyltransferase (Anderson et al 2000), the hypomethylated pre-tRNA i Met undergoes polyadenylation by Trf4p and degradation by the nuclear exosome (Kadaba et al 2004(Kadaba et al , 2006. As overexpression of LHP1, which encodes yeast La, results in increased levels of both pre-and mature tRNA i Met in trm6 strains (Anderson et al 1998), binding by La may interfere with polyadenylation and/or exonucleolytic degradation.…”

Crystal Structure of Thermus thermophilus tRNA m1A58 Methyltransferase and Biophysical Characterization of Its Interaction with tRNA

“…Actually, in the yeast Saccharomyces cerevisiae, mutants defective in N 1 -methylation of A 58 are non-viable. 2 Likewise, in Thermus thermophilus, gene disruption studies have shown that m 1 A 58 modification is required for growth of this bacterium at high temperatures. 3 S-Adenosyl-L-methionine (AdoMet) dependent methyltransferases (MTases) are involved in a wide variety of biological processes involving methylation of nucleic acids, proteins, phospholipids and small molecules using the ubiquitous methyl donor AdoMet.…”