The gene encoding the capsid protein P82 of the Choristoneura fumiferana multicapsid nucleopolyhedrovirus: sequencing, transcription and characterization by immunoblot analysis.

Identification of protein-protein interactions of the occlusion-derived virus-associated proteins of Helicoverpa armigera nucleopolyhedrovirus

“…3a, c, lane I). The appearance of a band migrating at a similar low molecular mass was also detected for VP80 of Choristoneura fumiferana MNPV (Li et al, 1997) when the sample from infected cells was probed with VP80 antibody. As this band was detected with anti-VP80 antisera, it might be due to the degradation of VP80.…”

Molecular analysis of the p48 gene of Choristoneura fumiferana multicapsid nucleopolyhedroviruses CfMNPV and CfDEFNPV.

“…The same immunological method was used successfully to detect the P82 protein in infected cells (Li et al, 1997). Thus, the amount of protein expressed in infected cells was probably below the threshold of immunoblot detection.…”

“…2 A). Immediately upstream of ORF p48 is the p82 gene, which encodes a capsid-related virus structural protein (Li et al, 1997). There are two other ORFs, p12 and p40, downstream of p48 in the fragment EcoRI-N (Fig.…”

Autographa californica Multiple Nucleopolyhedrovirus 38K Is a Novel Nucleocapsid Protein That Interacts with VP1054, VP39, VP80, and Itself

“…It was proposed that PP78/83, BV/ ODV-C42, and ODV-EC27 form a complex at the nucleocapsid base because electron microscopy revealed that PP78/83 is associated with the nucleocapsid base, and BV/ODV-C42 interacts directly with PP78/83 and ODV-EC27 (5,36,38). Several additional minor proteins have also been identified to be associated with the nucleocapsid, including PCNA (2), VP1054 (31), FP25 (4,18,35,48), VLF-1 (22,27,29,42,43,50), VP80 (21,25,30), and P24 (47). PP78/83, VP1054, and VLF-1 have been reported to be required for nucleocapsid assembly (31,34,43).…”