The genes encoding the P39 and CG30 proteins of Bombyx mori nuclear polyhedrosis virus

Lu, Maolong; Iatrou, Kostas

doi:10.1099/0022-1317-77-12-3135

Cited by 11 publications

(14 citation statements)

References 39 publications

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“…This may be necessary in some cell types that the virus infects. The ratios of IE0 and IE-1 also affect baculovirus host specificity (Lu and Iatrou, 1996;Pearson and Rohrmann, 1997;Stewart et al, 2005) and thus PNK/PNL may be playing a role in specificity through RNA splicing.…”

Section: H Nucleocapsid-associated Proteins Involved In Transcriptiomentioning

confidence: 99%

The Baculoviruses Occlusion‐Derived Virus: Virion Structure and Function

Slack

Arif

2006

Advances in Virus Research

264

240

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Section: H Nucleocapsid-associated Proteins Involved In Transcriptiomentioning

confidence: 99%

The Baculoviruses Occlusion‐Derived Virus: Virion Structure and Function

Slack

Arif

2006

Advances in Virus Research

264

240

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“…to deduce whether or not transcriptional activation of early (and late) BmNPV genes occurs. The RT-PCR analyses encompassed detection of transcripts originating from three early genes, ie1 (39), he65 (8), and ie0, the only baculovirus gene whose transcript contains an excisable intron (the splicing event involves the use of the splicing junction of the ie0 to the ie1 exon early in baculovirus infection and results in the addition of new N-terminal sequences to the IE1 transactivator [50]), as well as two late genes, p39 (53,75) and pol (41). Because of the lack of intronic sequences, which could enable an easy distinction between amplification of cDNA and genomic DNA, in all but the ie0 gene, all RNA preparations were treated with RNase-free DNase prior to PCR.…”

Section: Optimization Of Mammalian Cell Transduction Conditionsmentioning

confidence: 99%

Baculovirus-Mediated Gene Delivery into Mammalian Cells Does Not Alter Their Transcriptional and Differentiating Potential but Is Accompanied by Early Viral Gene Expression

Kenoutis

Efrose

Swevers

et al. 2006

J Virol

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During the last decade, it has become apparent that baculoviruses not only represent a powerful expression system for production of recombinant proteins in insect cells but also can be used for transduction of dividing and nondividing mammalian cells and tissues in vitro, ex vivo, and in vivo (49). Advantages of the use of baculoviruses as gene delivery agents include their inability to replicate in mammalian cells, apparent lack of cytotoxicity, capacity to sustain large insertions of foreign DNA, ability to target many different cell types, and superior safety features relative to mammalian virus-based transduction systems (25,37,38,48). Thus, increasing interest for the development of recombinant baculoviruses as gene delivery vectors for use in human gene therapy exists.All baculovirus vectors for gene delivery in mammalian cells reported thus far have been based on the Autographa californica nuclear polyhedrosis virus (AcNPV). This is primarily due to the relatively wide host range of the virus and the multiplicity of lepidopteran cell lines in which it can be grown and propagated effectively as well as the availability of integrated methodologies allowing the rapid generation of recombinant viruses (55, 80). In contrast, there is a paucity of information regarding the suitability of other baculovirus species, particularly species with narrow host ranges to function as transduction vectors for mammalian cells.In this regard, it is also known that AcNPV has a propensity to express endogenous viral genes in nonhost insect species (12, 13). Moreover, studies involving transfection of mammalian cells with gene constructs employing baculovirus gene promoter elements have shown that certain early baculovirus promoters are marginally functional in mammalian cells and can also be activated by mammalian virus regulators (46, 66). These findings raise safety concerns related to the potential for low-level baculovirus gene expression in mammalian cells and the triggering of cellular immune responses against the transduced cells by the recipient host (61).In this work, we have explored the capacity of Bombyx mori NPV (BmNPV), the second best characterized baculovirus species after AcNPV (2,20,21,27,40,56,57,65), which has a very limited host range (58), to function as a transducing vector for mammalian cell lines and primary Schwann cells. The choice for the latter rests with the results of experimental transplantation in rodent and primate models, which has provided substantial evidence that Schwann cells are good candidates for cell therapy in human central nervous system (CNS) demyelinating diseases, such as multiple sclerosis, and trauma (5,6,9,19,26). Schwann cells provide trophic support and remyelinate demyelinated CNS axons. However, their integration in the CNS is limited. Therefore, modifying Schwann cells to express "therapeutic" factors enhancing axonal regeneration and remyelination by ex vivo gene transduction is a promising strategy to improve their capacity to repair the injured or demyelinated nervous ...

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“…In AcMNPV and BmNPV, the cg30 promoter is shown to be present within the ORF of vp39, a major capsid protein gene (Thiem and Miller, 1989;Lu and Iatrou, 1996;Katsuma et al, 2011b). This suggests that insertional mutations of cg30 may have effect on vp39 transcription, presumably leading to aberrant BV and OB production.…”

Section: Discussionmentioning

confidence: 95%

“…The cg30 genes of Autographa californica NPV (AcMNPV) and Bombyx mori NPV (BmNPV) are expressed as delayed early genes (Thiem and Miller, 1989;Lu and Iatrou, 1996) and encode a 30-kDa protein with two putative functional domains, a RING finger motif and a leucine zipper motif (Thiem and Miller, 1989). To investigate the roles of cg30 during NPV infection, gene-knockout studies have been performed in AcMNPV and BmNPV.…”

Section: Introductionmentioning

confidence: 99%