The Genetics of Transfer RNA in Drosophila

Kubli, Eric

doi:10.1016/s0065-2660(08)60298-9

Cited by 27 publications

(9 citation statements)

References 152 publications

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“…Earlier studies suggested that su(s) affects tRNA modification (44,45) or that su(s) encodes a posttranslational inhibitor (46). However, our finding that the suppressible v alleles are insertions of the mobile element 412 illustrates that su(s) belongs to the class of Drosophila suppressors that suppress transposable element insertion mutations.…”

Section: Discussionmentioning

confidence: 55%

Molecular characterization of the Drosophila vermilion locus and its suppressible alleles.

Searles

Voelker

1986

Proc. Natl. Acad. Sci. U.S.A.

101

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We have cloned vermilion (v), one of the genes required for brown eye pigment synthesis in Drosophila, using a mutant (vH2a) "tagged" with the transposon P factor.Mutations that disrupt v gene expression are clustered within approximately 2 kilobases of DNA. A 1.4-kilobase transcript, homologous to this same region, is present in v+ RNA but absent in RNA from several v mutants. The spontaneous v alleles that are suppressed by the suppressor of sable [su(s) MATERIALS AND METHODSPurification of Nucleic Acids. The v mutants used for nucleic acid isolation are described by Lindsley and Grell (16) or are referenced in the text. Drosophila DNA was isolated from adult flies as described by Bingham et al. (18). Procedures for isolation ofbacteriophage X DNA and plasmid DNA have been described (19). Total RNA was prepared from 2-day-old adults by using procedures described by Thireos et al. (20) except that 10 mM vanadyl-ribonucleoside complex was included in the homogenization buffer. Poly(A)+ RNA was purified by chromatography on oligo(dT)-cellulose (21 nitrocellulose (26). The hybridization conditions were the same as described for Southern transfers. The hybridization probes were plasmid subclones that had been labeled with 32P by nick-translation (27).Abbreviations: kb, kilobase(s); bp, base pair(s). 404The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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Section: Discussionmentioning

confidence: 55%

Molecular characterization of the Drosophila vermilion locus and its suppressible alleles.

Searles

Voelker

1986

Proc. Natl. Acad. Sci. U.S.A.

101

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“…Although there is a rough correlation between the number of tRNA genes and the “complexity” of an organism, the actual number of tRNA genes [ 74 ] is not directly proportional to the genome size as can be exemplified when one looks at diptera. While D. melanogaster , with a genome size of ~125 Mb contains between 590 and 950 tRNA genes [ 75 ]. Aedes aegypti (genome size: ~1.3 Gb) contains 906 and A. gambiae 441 with a ~270 Mb long genome [ 76 ].…”

Section: Resultsmentioning

confidence: 99%

Non-coding RNA gene families in the genomes of anopheline mosquitoes

et al. 2014

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BackgroundOnly a small fraction of the mosquito species of the genus Anopheles are able to transmit malaria, one of the biggest killer diseases of poverty, which is mostly prevalent in the tropics. This diversity has genetic, yet unknown, causes. In a further attempt to contribute to the elucidation of these variances, the international “Anopheles Genomes Cluster Consortium” project (a.k.a. “16 Anopheles genomes project”) was established, aiming at a comprehensive genomic analysis of several anopheline species, most of which are malaria vectors. In the frame of the international consortium carrying out this project our team studied the genes encoding families of non-coding RNAs (ncRNAs), concentrating on four classes: microRNA (miRNA), ribosomal RNA (rRNA), small nuclear RNA (snRNA), and in particular small nucleolar RNA (snoRNA) and, finally, transfer RNA (tRNA).ResultsOur analysis was carried out using, exclusively, computational approaches, and evaluating both the primary NGS reads as well as the respective genome assemblies produced by the consortium and stored in VectorBase; moreover, the results of RNAseq surveys in cases in which these were available and meaningful were also accessed in order to obtain supplementary data, as were “pre-genomic era” sequence data stored in nucleic acid databases. The investigation included the identification and analysis, in most species studied, of ncRNA genes belonging to several families, as well as the analysis of the evolutionary relations of some of those genes in cross-comparisons to other members of the genus Anopheles.ConclusionsOur study led to the identification of members of these gene families in the majority of twenty different anopheline taxa. A set of tools for the study of the evolution and molecular biology of important disease vectors has, thus, been obtained.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-1038) contains supplementary material, which is available to authorized users.

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“…Indeed, any mechanism of translational suppression of insertion mutations within coding regions seems unlikely. It may also be noted that no tRNA hybridization is seen at 88B, the location of su(Hw) (32). In the separate case of suppressor of sable, su(s), there is evidence that suppression affects RNA modification (32) and enzyme inactivation (33).…”

mentioning

confidence: 98%

“…It may also be noted that no tRNA hybridization is seen at 88B, the location of su(Hw) (32). In the separate case of suppressor of sable, su(s), there is evidence that suppression affects RNA modification (32) and enzyme inactivation (33). Nevertheless, the mechanism of suppression has not been fully established for this or any other Drosophila suppressor system.…”

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confidence: 99%

Drosophila melanogaster mutations suppressible by the suppressor of Hairy-wing are insertions of a 7.3-kilobase mobile element.

Modolell¹,

Bender²,

Meselson³

1983

Proc. Natl. Acad. Sci. U.S.A.

276

120

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Certain spontaneous mutations of Drosophila melanogaster are suppressed by su(Hw), the suppressor of Hairywing (3R-54.8). We find that mutations suppressible by su(Hw) result from insertions of a mobile element at the affected loci. The element, named gypsy, is approximately 7.3 kilobases long and includes 0.5-kilobase direct terminal repeats. It was first identified in DNA cloned from the bithorax chromosomal region of several Drosophila stocks carrying suppressible mutations of the bithorax complex. Cloned gypsy DNA was used as a probe to test for the association of gypsy with suppressible mutations at various other loci by hybridization in situ. Gypsy was found to be associated with 19 suppressible alleles at 10 different loci: yellow, Hairy-wing, scute, diminutive, cut, lozenge, forked, Beadex, hairy, and the bithorax complex. It was not found with wild-type or nonsuppressible mutations at any of these loci. Gypsy DNA was also used as a probe to clone the element and adjacent unique DNA from the loci of some suppressible mutations. This confirmed the presence of the full-length element and also provided cloned DNA from the previously uncloned loci scute and cut. The suppressor of Hairy-wing is generally recessive and behaves as a null mutation. Thus, the disruption of normal gene function caused by the inserted gypsy element appears to require some product of the wild-type suppressor gene, su(Hw)+.

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The Genetics of Transfer RNA in Drosophila

Cited by 27 publications

References 152 publications

Molecular characterization of the Drosophila vermilion locus and its suppressible alleles.

Molecular characterization of the Drosophila vermilion locus and its suppressible alleles.

Non-coding RNA gene families in the genomes of anopheline mosquitoes

Drosophila melanogaster mutations suppressible by the suppressor of Hairy-wing are insertions of a 7.3-kilobase mobile element.

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