The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1

Rasko, David A.; Ravel, Jacques; Økstad, Ole Andreas; Helgason, Erlendur; Cer, Regina Z.; Jiang, Lingxia; Shores, Kelly A.; Fouts, Derrick E.; Tourasse, Nicolas J.; Angiuoli, Samuel V.; Kolonay, James F.; Nelson, Karen E.; Kolstø, Anne‐Brit; Fraser, Claire M.; Read, Timothy D.

doi:10.1093/nar/gkh258

Cited by 280 publications

(285 citation statements)

References 77 publications

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“…To assess the mammalian virulence of B. cereus G9241, A͞J mice were challenged by i.p. injection (21) with mature spores of B. anthracis Sterne, B. cereus G9241, and B. cereus ATCC10987 (12). In these preliminary studies, G9241 produced 100% lethality, consistent with the positive control B. anthracis Sterne, although lethality was achieved faster when the high-spore dose was inoculated (t test, P ϭ 0.081) (Fig.…”

Section: Isolation Of B Cereus G9241 During a Retrospective Analysimentioning

confidence: 53%

“…Although genomic sequences with homology to B. anthracis pXO1 and pXO2 sequences have been detected in other Bacillus spp. (12), this work reports on the toxin genes and an entire pXO1-like plasmid found outside of a B. anthracis isolate.…”

Section: Discussionmentioning

confidence: 99%

“…A 13.5ϫ coverage draft genome sequence was obtained and assembled by using the Celera assembler (11). The assembly consisted of 131 contigs that were ordered and concatenated with our prior knowledge of the B. anthracis and B. cereus genome sequences (10,12). The resulting 5,286,464-bp sequence was annotated through the Institute for Genomic Research Bioinformatics pipeline as reported (13).…”

Section: Methodsmentioning

confidence: 99%

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Identification of anthrax toxin genes in aBacillus cereusassociated with an illness resembling inhalation anthrax

Hoffmaster

Ravel

Rasko

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

444

537

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Bacillus anthracis is the etiologic agent of anthrax, an acute fatal disease among mammals. It was thought to differ from Bacillus cereus, an opportunistic pathogen and cause of food poisoning, by the presence of plasmids pXO1 and pXO2, which encode the lethal toxin complex and the poly-␥-D-glutamic acid capsule, respectively. This work describes a non-B. anthracis isolate that possesses the anthrax toxin genes and is capable of causing a severe inhalation anthrax-like illness. Although initial phenotypic and 16S rRNA analysis identified this isolate as B. cereus, the rapid generation and analysis of a high-coverage draft genome sequence revealed the presence of a circular plasmid, named pBCXO1, with 99.6% similarity with the B. anthracis toxin-encoding plasmid, pXO1. Although homologues of the pXO2 encoded capsule genes were not found, a polysaccharide capsule cluster is encoded on a second, previously unidentified plasmid, pBC218. A͞J mice challenged with B. cereus G9241 confirmed the virulence of this strain. These findings represent an example of how genomics could rapidly assist public health experts responding not only to clearly identified select agents but also to novel agents with similar pathogenic potentials. In this study, we combined a public health approach with genome analysis to provide insight into the correlation of phenotypic characteristics and their genetic basis.

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Section: Isolation Of B Cereus G9241 During a Retrospective Analysimentioning

confidence: 53%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Identification of anthrax toxin genes in aBacillus cereusassociated with an illness resembling inhalation anthrax

Hoffmaster

Ravel

Rasko

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

444

537

View full text Add to dashboard Cite

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“…Although the B. cereus group has received much attention in the past few years and many B. cereus group genomes have recently been sequenced and published (Han et al, 2006;Ivanova et al, 2003;Rasko et al, 2004;Read et al, 2002Read et al, , 2003, hardly any research has been done on TCSs in this bacterial group. Only recently, a number of HKs has been shown to initiate sporulation in B. anthracis (Brunsing et al, 2005) and a RR has been shown to activate the alternative sigma factor s B in B. cereus (van Schaik et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of two-component signal transduction systems of Bacillus cereus, Bacillus thuringiensis and Bacillus anthracis

et al. 2006

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Members of the Bacillus cereus group are ubiquitously present in the environment and can adapt to a wide range of environmental fluctuations. In bacteria, these adaptive responses are generally mediated by two-component signal transduction systems (TCSs), which consist of a histidine kinase (HK) and its cognate response regulator (RR). With the use of in silico techniques, a complete set of HKs and RRs was recovered from eight completely sequenced B. cereus group genomes. By applying a bidirectional best-hits method combined with gene neighbourhood analysis, a footprint of these proteins was made. Around 40 HK-RR gene pairs were detected in each member of the B. cereus group. In addition, each member contained many HK and RR genes not encoded in pairs (‘orphans’). Classification of HKs and RRs based on their enzymic domains together with the analysis of two neighbour-joining trees of these domains revealed putative interaction partners for most of the ‘orphans’. Putative biological functions, including involvement in virulence and host–microbe interactions, were predicted for the B. cereus group HKs and RRs by comparing them with those of B. subtilis and other micro-organisms. Remarkably, B. anthracis appeared to lack specific HKs and RRs and was found to contain many truncated, putatively non-functional, HK and RR genes. It is hypothesized that specialization of B. anthracis as a pathogen could have reduced the range of environmental stimuli to which it is exposed. This may have rendered some of its TCSs obsolete, ultimately resulting in the deletion of some HK and RR genes.

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“…Also, the sequence data of phaR was 484 bp and the BLAST search of homologous sequence to PHAR showed 99% homology to PHA synthase subunit PHAR; regulator of PhaP of Bacillus cereus ATCC 10987, accession No. : NC_003909.8 [22] and Bacillus thuringiensis serovar finitimus YBT-020, accession No. : NC_017200.1.…”

Section: Discussionmentioning

confidence: 99%

Isolation, Cloning and Sequencing of Poly 3-Hydroxybutyrate Synthesis Genes from Local Strain of Bacillus cereus mm7 and Expression them in E. coli

Rabey¹

2017

JIG

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Poly 3-hydroxybutyrate (PHB) is the most known degradable biopolymers, produced by some genera of bacteria under unfavorable growth conditions. Isolation, cloning, and expression of PHB genes from PHB producing Bacillus cereus was achieved. A highest Poly 3-hydroxybutyrate producer bacterium was selected and identified as Bacillus cereus MM7, based on morphological and physiological characters in addition to 16S rRNA gene sequences. The four genes of Bacillus cereus MM7 that involved in Poly 3-hydroxybutyrate production were successfully amplified using specific primers which were designed depending on the sequence alignment of the known PHA biosynthetic genes from Bacillus cereus, cloned in E. coli DH5α and completely sequenced. Moreover, the expression of the four genes responsible for PHA biosynthetic in E. coli XL1-Blue and E coli MJ 109 was assayed by 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis. In the current study Bacillus cereus was able to synthesize PHB. PHB genes were isolated, cloned and expressed in E. coli DH5α. The PHB encoding proteins were analyzed in SDS-PAGE and the PHB corresponding protein bands were analyzed using mass spectrometry to nominate their amino acid sequence. Keywords: Pre cultureThe pre culture was prepared in 250 ml Erlenmeyer flasks containing 50 ml of nutrient broth [16]. Each flask was inoculated with a loop of the tested cultures and incubated in a 130 rpm speed rotary shaker at 30˚C for 24h.

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The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1

Cited by 280 publications

References 77 publications

Identification of anthrax toxin genes in aBacillus cereusassociated with an illness resembling inhalation anthrax

Identification of anthrax toxin genes in aBacillus cereusassociated with an illness resembling inhalation anthrax

Comparative analysis of two-component signal transduction systems of Bacillus cereus, Bacillus thuringiensis and Bacillus anthracis

Isolation, Cloning and Sequencing of Poly 3-Hydroxybutyrate Synthesis Genes from Local Strain of Bacillus cereus mm7 and Expression them in E. coli

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