The genus Anoxybacillus: an emerging and versatile source of valuable biotechnological products

Rosa, Deisiane Fernanda da; Macêdo, Alexandre José

doi:10.1007/s00792-023-01305-3

Cited by 4 publications

(3 citation statements)

References 112 publications

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“…The genus Anoxybacillus , initially characterized by Pikuta in 2000 [ 59 ], which originally consisted of 2 species, Anoxybacillus pushchinensis and Anoxybacillus flavithermus , has expanded to encompass 24 species and two subspecies as of 2023 [ 60 ]. This genus comprises thermophilic and alkaliphilic microorganisms and has been isolated from diverse locations worldwide [ 61 , 62 ].…”

Section: Discussionmentioning

confidence: 99%

“…The inoculation of isolated strains was performed using fresh cultures, and the cell count was conducted based on a minimum of 300 cells observed in at least 20 random fields per sample [30]. The strains were cultured in triplicates of 3 mL of liquid ATM1 medium, exposed to temperatures ranging from 37 to 80 • C (37,40,45,50,55,60,65,70,75, and 80 • C), and subjected to different pH conditions using various buffers (50 mM Acetic Acid-Acetate for pH 5, 50 mM HEPES for pH 6 and 7, 50 mM Tris-HCl for pH 8, and 50 mM NaHCO 3 -Na 2 CO 3 for pH 9 and 10). This was done to determine the optimal pH and growth temperature for the strains.…”

Section: Determination Of Cultivation Parametersmentioning

confidence: 99%

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Isolation of Thermophilic Bacteria from Extreme Environments in Northern Chile

Valenzuela,

Solís-Cornejo,

Araya

et al. 2024

Microorganisms

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The northern region of Chile boasts unique geographical features that support the emergence of geothermal effluents, salt lagoons, and coastal creeks. These extreme climate conditions create polyextreme habitats for microorganisms, particularly adapted to survive these harsh environments. These extremophilic microorganisms hold immense potential as a source of hydrolytic enzymes, among other biotechnological applications. In this study, we isolated 15 strains of aerobic thermophilic bacteria (45–70 °C) from sediment samples collected at five different ecological sites, including hot springs, geothermal fields, and lagoons in the Atacama Desert and Andes high planes. Analyses of the 16S rRNA gene sequences of the isolates showed a close genetic similarity (98–100%) with microorganisms of the genera Parageobacillus, Geobacillus, Anoxybacillus, and Aeribacillus. Notably, these thermophiles exhibited significant hydrolytic enzyme activity, particularly amylases, lipases, and proteases. These findings underscore the potential of using these thermophilic bacterial strains as an invaluable source of thermozymes with wide-ranging applications in diverse industries, such as detergent formulations, pharmaceutical processing, and food technology. This research highlights the ecological significance of these extreme environments in the Atacama Desert and Andes high plains, which serve as vital ecological niches housing extremophilic bacteria as a genetic source of relevant thermozymes, promising great potential for innovation in the biotechnology industry.

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Section: Discussionmentioning

confidence: 99%

Section: Determination Of Cultivation Parametersmentioning

confidence: 99%

Isolation of Thermophilic Bacteria from Extreme Environments in Northern Chile

Valenzuela,

Solís-Cornejo,

Araya

et al. 2024

Microorganisms

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“…Anoxybacillus species are frequently discovered in thermophilic conditions. As an example, Anoxybacillus flavithermus inhabits hot springs and is characterized as a Grampositive, endospore-forming anaerobic microorganism [36]. To date, there are only seven whole genomes for Anoxybacillus flavithermus strains deposited in genome databases.…”

Section: Introductionmentioning

confidence: 99%

Expression and Functional Analysis of the Compact Thermophilic Anoxybacillus flavithermus Cas9 Nuclease

Matveeva,

Ryabchenko,

Petrova

et al. 2023

IJMS

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Research on Cas9 nucleases from different organisms holds great promise for advancing genome engineering and gene therapy tools, as it could provide novel structural insights into CRISPR editing mechanisms, expanding its application area in biology and medicine. The subclass of thermophilic Cas9 nucleases is actively expanding due to the advances in genome sequencing allowing for the meticulous examination of various microorganisms’ genomes in search of the novel CRISPR systems. The most prominent thermophilic Cas9 effectors known to date are GeoCas9, ThermoCas9, IgnaviCas9, AceCas9, and others. These nucleases are characterized by a varying temperature range of the activity and stringent PAM preferences; thus, further diversification of the naturally occurring thermophilic Cas9 subclass presents an intriguing task. This study focuses on generating a construct to express a compact Cas9 nuclease (AnoCas9) from the thermophilic microorganism Anoxybacillus flavithermus displaying the nuclease activity in the 37–60 °C range and the PAM preference of 5′-NNNNCDAA-3′ in vitro. Here, we highlight the close relation of AnoCas9 to the GeoCas9 family of compact thermophilic Cas9 effectors. AnoCas9, beyond broadening the repertoire of Cas9 nucleases, suggests application in areas requiring the presence of thermostable CRISPR/Cas systems in vitro, such as sequencing libraries’ enrichment, allele-specific isothermal PCR, and others.

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