The Genus Aquaspirillum

Pot, Bruno; Gillis, Monique; Ley, J. De

doi:10.1007/0-387-30745-1_30

Cited by 5 publications

(4 citation statements)

References 38 publications

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“…No other pure culture of the Aquaspirillum ‐related bacteria exists, so this study presents the first more detailed study of the physiology of these organisms. The closest isolated relatives are Aquaspirillum delicatum and Pseudomonas lanceolata , which grow on a variety of substrates under aerobic conditions, but are unable to perform full denitrification (the strains can only make a partial reduction of nitrate to nitrite; Leifson, 1962; Krieg, 1984; Pot et al , 1991). This physiology is very different from that found in this in situ study of the Aquaspirillum ‐related bacteria, where only uptake of phenylalanine and perhaps a few other amino acids were observed and they most likely carried out full denitrification.…”

Section: Discussionmentioning

confidence: 99%

Ecophysiology of abundant denitrifying bacteria in activated sludge

Thomsen

Kong

Nielsen

2007

FEMS Microbiology Ecology

191

108

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The abundance of potential denitrifiers in full-scale wastewater treatment plants with biological nitrogen and phosphorus removal was investigated by FISH and various oligonucleotide probes. The potential denitrifiers were characterized as probe-defined populations that were able to consume radiolabelled substrate with oxygen, nitrate and nitrite as electron acceptor as determined by microautoradiography. The most abundant potential denitrifiers were related to the genera Aquaspirillum, Azoarcus, Thauera and Rhodocyclus, all within the Betaproteobacteria. They made up 20-49% of all bacteria in most of the 17 nitrogen removal plants investigated and were hardly present in four plants without denitrification. The ecophysiology of Aquaspirillum, Azoarcus and Thauera-related bacteria was consistent within each probe-defined group in the plants investigated. These three groups showed distinct physiological differences, with the Aquaspirillum-related bacteria appearing as the most specialized one, consuming only amino acids among the substrates tested, and Thauera as the most versatile consuming some volatile fatty acids, ethanol and amino acids. The coexistence of Aquaspirillum, Azoarcus and Thauera-related bacteria in a range of treatment plants with differences in wastewater, design and operation suggest that the populations ensure a functional stability of the plants by occupying different ecological niches related to the carbon transformation.

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Section: Discussionmentioning

confidence: 99%

Ecophysiology of abundant denitrifying bacteria in activated sludge

Thomsen

Kong

Nielsen

2007

FEMS Microbiology Ecology

191

108

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“…The closest isolated relatives to the Aquaspi‐sequences, Aquaspirillum delicatum and Pseudomonas lanceolata, are heterotrophs, grow under aerobic conditions, but are unable to perform full denitrification (can reduce nitrate to nitrite) (Leifson, 1962; Krieg, 1984; Pot et al ., 1991). Clone GOUTB19 and GOUTB4 from Alfreider et al .…”

Section: Discussionmentioning

confidence: 99%

Micromanipulation and further identification of FISH‐labelled microcolonies of a dominant denitrifying bacterium in activated sludge

Thomsen

Nielsen

Ramsing

et al. 2004

Environmental Microbiology

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The activated sludge process relies on the formation of strong microbial flocs. The knowledge about dominant floc-forming bacteria is at present very limited, especially from a phylogenetic perspective. In this study, numerous microcolonies in the activated sludge flocs were found to be targeted by a Betaproteobacteria-group-specific oligonucleotide probe using fluorescence in situ hybridization (FISH). Some of these were micromanipulated and further identified by reverse transcriptase polymerase chain reaction (RT-PCR) and sequencing to belong to the Aquaspirillum genus in the Neisseriaceae family. A specific oligonucleotide probe, Aqs997, was designed to target the identified bacteria. A survey in nine different wastewater treatment plants with nutrient removal (WWTP) showed a high abundance of bacteria hybridizing to the oligonucleotide probe developed. Microautoradiography (MAR) combined with FISH on activated sludge incubated with radiolabelled substrate showed uptake of substrate with oxygen, nitrate and nitrite as electron acceptor demonstrating a denitrifying potential of the bacteria investigated. The Aquaspirillum-related bacteria seemed to be abundant denitrifiers in WWTPs with nitrogen removal and they were particularly numerous in plants mainly receiving domestic wastewater, where they constituted up to 30% of all bacteria.

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“…Aquaspirillum delicatum y Methylibium petroleiphilum, agrupadas en el clado II, tienen la capacidad de regular metabólicamente la fijación y el uso de amonio en el medio de cultivo (Pot et al, 2006;Cheng et al, 2011). Esto resulta importante porque la nitrogenasa puede inhibirse por concentraciones de amonio superiores a 18 mg L -1 y de nitrato superiores a 1,240 mg L -1 (Almon & Boger, 1988;Li et al, 2010).…”

Section: Abundancia Relativaunclassified

Phylogenetic and morphological identification of a photosynthetic microbial consortium of potential biotechnological interest

et al. 2016

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morfológica y filogenética de un consorcio microbiano fotosintético de posible interés biotecnológico. Hidrobiológica 26 (2): 311-321. RESUMEN Antecedentes. Los consorcios microbianos son de importancia ecológica y biotecnológica por que contribuyen a los ciclos biogeoquímicos y producen compuestos de alto valor añadido. Objetivos. Realizar el estudio polifásico de un consorcio microbiano (CM) fotosintético, con el fin de identificar los microorganismos que lo integran, además del aporte teórico del potencial biotecnológico de cada uno de ellos como parte del consorcio. Métodos. Se empleó un estudio de la diversidad morfológica y filogenética. Resultados. En el CM se identificaron 21 microorganismos diferentes pertenecientes a los phyla Proteobacteria (Rhodobacter sp., Devosia insulae, Pedomicrobium americanum, Alpha proteobacteria, Aquaspirillum delicatum, Methylibium petroleiphilum y Nannocystis sp.), Bacteroidetes (Flavobacterium sp. y Flavobacterium aquatile), Cyanobacteria (Aphanizomenon aphanizomenoides, Leptolyngbya sp. y Anabaena oscillarioides), Chlorophyta (Monoraphidium sp., Chlorella sp.), Heterokontophyta (Cyclotella meneghiniana, Melosira varians, Cocconeis placentula, Achnanthidium chlidanos, Navicula radiosa, Fragilaria ulna y Nitzschia sp.). Este consorcio microbiano ha mostrado tener una elevada capacidad de fijación de nitrógeno (10,294 nmoles etileno g-1 peso seco h-1). Conclusiones. La identificación de los microorganismos que conforman el CM, su capacidad de crecimiento y la fijación de nitrógeno en un fotobiorreactor, permiten vislumbrar su posible aplicación biotecnológica como biofertilizante.

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The Genus Aquaspirillum

Cited by 5 publications

References 38 publications

Ecophysiology of abundant denitrifying bacteria in activated sludge

Ecophysiology of abundant denitrifying bacteria in activated sludge

Micromanipulation and further identification of FISH‐labelled microcolonies of a dominant denitrifying bacterium in activated sludge

Phylogenetic and morphological identification of a photosynthetic microbial consortium of potential biotechnological interest

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