The genus <i>Micromonospora</i> as a model microorganism for bioactive natural product discovery

Hifnawy, Mohamed S.; Fouda, Mohamed; Sayed, Ahmed M.; Mohammed, Rabab; Hassan, Hossam M.; AbouZid, Sameh; Rateb, Mostafa E.; Keller, Alexander; Adámek, M.; Ziemert, Nadine; Abdelmohsen, Usama Ramadan

doi:10.1039/d0ra04025h

Cited by 41 publications

(37 citation statements)

References 157 publications

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“…The next top metabolite linked to the antitrypanosomal activity was antascomicin C (28) (accurate mass = 689.4139, p = 2.66 × 10 −4 ), an antibiotic previously reported from several Micromonospora strains [1,41].…”

Section: Bioactivity-metabolites Correlationmentioning

confidence: 96%

“…UR17, but did not show a specific cluster ( Figure 2). Micromonospora is a well-known talented actinomycete and was considered as a huge pipeline of diverse, interesting bioactive specialized metabolites during the last 60 years; it is still considered as an untapped source for further novel ones [1]. Hence, this new strain of Micromonospora can be considered a promising source of bioactive chemical entities.…”

Section: Identification and Phylogenetic Analysis Of The Isolated Actmentioning

confidence: 99%

“…UR17-derived metabolite neihumicin (26) (accurate mass = 304.1205, p = 3.11 × 10 −3 ). This metabolite has been associated with interesting cytotoxicity against a wide range of cancer cell lines [1,45].…”

Section: Bioactivity-metabolites Correlationmentioning

confidence: 99%

“…Actinomycetes are considered talented producers of bioactive secondary metabolites that are spread across a wide range of terrestrial and aquatic habitats. These Gram-positive bacteria can also establish symbiotic relationships with other higher organisms like plants, animals, and insects [1][2][3]. During the last 70 years, actinomycetes from terrestrial environments have been extensively investigated and have gained great success in providing much essential therapeutics (e.g., antibiotics and anticancer agents).…”

Section: Introductionmentioning

confidence: 99%

“…Induced metabolites(1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18) of Streptomyces sp. UR23-derived extracts: upon fermentation on the solid medium (black color, 1-4), on the liquid medium (blue color, 5-9), upon the co-fermentation on the solid medium (red color, 10-13), and upon the co-fermentation in the liquid medium (brown color, 14-18).…”

mentioning

confidence: 99%

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Exploration of Chemical Diversity and Antitrypanosomal Activity of Some Red Sea-Derived Actinomycetes Using the OSMAC Approach Supported by LC-MS-Based Metabolomics and Molecular Modelling

et al. 2020

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In the present study, we investigated the actinomycetes associated with the Red Sea-derived soft coral Sarcophyton glaucum in terms of biological and chemical diversity. Three strains were cultivated and identified to be members of genera Micromonospora, Streptomyces, and Nocardiopsis; out of them, Micromonospora sp. UR17 was putatively characterized as a new species. In order to explore the chemical diversity of these actinobacteria as far as possible, they were subjected to a series of fermentation experiments under altering conditions, that is, solid and liquid fermentation along with co-fermentation with a mycolic acid-containing strain, namely Nocardia sp. UR23. Each treatment was found to affect these actinomycetes differently in terms of biological activity (i.e., antitrypanosomal activity) and chemical profiles evidenced by LC-HRES-MS-based metabolomics and multivariate analysis. Thereafter, orthogonal projections to latent structures discriminant analysis (OPLS-DA) suggested a number of metabolites to be associated with the antitrypanosomal activity of the active extracts. The subsequent in silico screenings (neural networking-based and docking-based) further supported the OPLS-DA results and prioritized desferrioxamine B (3), bafilomycin D (10), and bafilomycin A1 (11) as possible antitrypanosomal agents. Our approach in this study can be applied as a primary step in the exploration of bioactive natural products, particularly those from actinomycetes.

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Section: Bioactivity-metabolites Correlationmentioning

confidence: 96%

Section: Identification and Phylogenetic Analysis Of The Isolated Actmentioning

confidence: 99%

Section: Bioactivity-metabolites Correlationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Exploration of Chemical Diversity and Antitrypanosomal Activity of Some Red Sea-Derived Actinomycetes Using the OSMAC Approach Supported by LC-MS-Based Metabolomics and Molecular Modelling

et al. 2020

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Yield improvement of enediyne yangpumicins in Micromonospora yangpuensis through ribosome engineering and fermentation optimization

Wang

Sun

et al. 2021

Biotechnology Journal

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Yangpumicins (YPMs), for example, YPM A, F, and G, are newly discovered enediynes from Micromonospora yangpuensis DSM 45577, which could be exploited as promising payloads of antibody-drug conjugates. However, the low yield of YPMs in the wildtype strain (∼1 mg L −1 ) significantly hampers their further drug development. In this study, a combined ribosome engineering and fermentation optimization strategy has been used for yield improvement of YPMs. One gentamicin-resistant M. yangpuensis DSM 45577 strain (MY-G-1) showed higher YPMs production (7.4 ± 1.0 mg L −1 ), while it exhibits delayed sporulation and slender mycelium under scanning electron microscopy. Whole genome re-sequencing of MY-G-1 reveals several deletion and single nucleotide polymorphism mutations, which were confirmed by PCR and DNA sequencing. Further Box-Behnken experiment and regression analysis determined that the optimal medium concentrations of soluble starch, D-mannitol, and pharmamedia for YPMs production in shaking flasks (10.0 ± 0.8 mg L −1 ). Finally, the total titer of YPM A/F/G in MY-G-1 reached to 15.0 ± 2.5 mg L −1 in 3 L fermenters, which was about 11-fold higher than the original titer of 1.3 ± 0.3 mg L −1 in wild-type strain. Our study may be instrumental to develop YPMs into a clinical anticancer drug, and inspire the use of these multifaceted strategies for yield improvement in Micromonospora species.

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Determination of Bacterial Diversity of Propolis Microbiota

Sudağıdan

Omeroglu

Arserim-Uçar

et al. 2023

Chemistry & Biodiversity

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Propolis is a natural resinous mixture produced by the excretions of honeybees. PCR amplification of the 16S rRNA gene region was achieved using DNA of pre-enriched propolis samples collected from Apis mellifera production hives (n = 37) in Eastern Türkiye (Bingöl and its regions). Next-generation sequencing and metabarcoding techniques were used to identify bacterial communities in propolis samples. Firmicutes dominated the phylum structure, with Proteobacteria, Actinobacteria, Tenericutes, and Spirochaetes following. The top three bacterial families were Bacillaceae, Enterobacteriaceae, and Enterococcaceae. Bacillus (dominantly B. badius and B. thermolactis at the species level) was recognized at the genus level, followed by Enterococcus and Clostridium sensu stricto. Our study comprehensively identified the bacterial diversity of propolis samples. Further investigations targeting to enlighten the microbiota of propolis and its potential application fields are required to gain better insight into ecological, nutritional, and medicinal perspectives.

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The genus Micromonospora as a model microorganism for bioactive natural product discovery

Abstract: We explore the genus Micromonospora as a model for natural product research and the discovery progress from the classical bioassay-guided approaches through to the application of genome mining and genetic engineering techniques that target specific products.

Cited by 41 publications

References 157 publications

Exploration of Chemical Diversity and Antitrypanosomal Activity of Some Red Sea-Derived Actinomycetes Using the OSMAC Approach Supported by LC-MS-Based Metabolomics and Molecular Modelling

Exploration of Chemical Diversity and Antitrypanosomal Activity of Some Red Sea-Derived Actinomycetes Using the OSMAC Approach Supported by LC-MS-Based Metabolomics and Molecular Modelling

Yield improvement of enediyne yangpumicins in Micromonospora yangpuensis through ribosome engineering and fermentation optimization

Determination of Bacterial Diversity of Propolis Microbiota

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