The germ-free mice monocolonization with <i>Bacteroides fragilis</i> improves azoxymethane/dextran sulfate sodium induced colitis-associated colorectal cancer

Lee, Yen Peng; Chiu, Chien Chao; Lin, T.; Hung, Shao Wen; Huang, Wen Ching; Chiu, Ching-Feng; Huang, Yen Te; Chen, Yi Hsun; Chen, Ter Hsin; Chuang, Hsiao-Li

doi:10.1080/08923973.2019.1569047

Cited by 16 publications

(12 citation statements)

References 37 publications

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“…The anti-inflammatory and anti-tumorigenic effects against CAC in BF gnotobiotic micewere noted in our previous report. 19 Here, the anti-tumorigenic effect of the tumor mass in histological colonic adenoma was shown only in the BF/WT group rather than BF/T4K group. These findings suggest that effect of BF mono- inoculation against CAC might be mediated by TLR4 signaling.…”

Section: Discussionmentioning

confidence: 64%

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Toll-like receptor 4 prevents AOM/DSS-induced colitis-associated colorectal cancer in Bacteroides fragilis gnotobiotic mice

et al. 2020

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Bacteroides fragilis (BF) plays a critical role in developing and maintaining the mammalian immune system. We previously found that BF colonization could prevent inflammation and tumor formation in a germ-free (GF) colitis-associated colorectal cancer (CAC) mouse model. The role of Toll-like receptor 4 (TLR4) in CAC development has not been clearly elucidated in BF mono-colonized gnotobiotic mice. The wild-type (WT) and TLR4 knockout (T4K) germ-free mice were raised with or without BF colonization for 28 days (GF/WT, GF/T4K, BF/WT, and BF/T4K) and then CAC was induced under azoxymethane (AOM)/dextran sulfate sodium (DSS) administration. The results showed that tumor formation and tumor incidence were significantly inhibited in the BF/WT group compared to those observed in the GF/WT group. However, the tumor prevention effect was not observed in the BF/T4K group unlike in the BF/WT group. Moreover, the CAC histological severity of the BF/WT group was ameliorated, but more severe lesions were found in the GF/WT, GF/T4K, and BF/T4K groups. Immunohistochemistry showed decreased cell proliferation (PCNA, β-catenin) and inflammatory markers (iNOS) in the BF/WT group compared to those in the BF/T4K group. Taken together, BF mono-colonization of GF mice might prevent CAC via the TLR4 signal pathway.

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Section: Discussionmentioning

confidence: 64%

“…The result was slightly different in a previous study, which had 33% incidence of adenocarcinoma in GF/WT mice. 19 We suspect that the different results might be due to shortening experimental period from 27 weeks to 16 weeks. In the BF/T4K group, the tumor incidence was as high as in GF/WT and GF/T4K mice (100%).…”

Section: Discussionmentioning

confidence: 99%

Toll-like receptor 4 prevents AOM/DSS-induced colitis-associated colorectal cancer in Bacteroides fragilis gnotobiotic mice

et al. 2020

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Section: Discussionmentioning

confidence: 99%

Gut Microbes and Microbial Metabolites in Colorectal Cancer Complicated with Different Serum Albumin Levels

Han

Wei

et al. 2020

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Background Patients with colorectal cancer (CRC) are at risk of malnutrition. Gut microbes and microbial metabolites are involved in the initiation and development of CRC. Purpose To investigate serum protein levels in CRC patients and explore the role of gut microbes and microbial metabolites in CRCs complicated with different serum albumin levels. Methods Overall, 398 CRC patients and same number of healthy volunteers in Huzhou Central Hospital from January 2016 to December 2018 were recruited to compare serum protein levels. The serological indicators were detected by Abbott Automatic Biochemical Analyzer(HCHL-YQ-SH-01). A total of 30 and 56 stool samples from CRC patients were used to detect intestinal microbes and microbial metabolites, respectively. Bacterial 16S V3-V4 and fungal ITS ribosomal DNA genes were sequenced and gas chromatography/mass spectrometry (GC/MS) was performed to detect microbial metabolites. Results Some serum protein-related indicators in the CRC group were lower than those in the control group (p༜0.05). The total protein and albumin levels in colon cancer patients were lower than those in rectal cancer patients (p༜0.05). The higher abundance of Sutterella is correlated with lower serum albumin level in CRCs. There were statistically significant differences in the abundance of fungi including Agaricomycetes, Simplicillium, Sclerotiniaceae, and Exophiala among patients with different serum albumin levels. Multiple gut bacteria and fungi are closely related to serum albumin levels.We found some characteristic microbial metabolites in CRCs complicated with different serum protein levels. Conclusions The different serum albumin levels were associated with the gut microbes and microbial metabolites in CRCs. It may provide novel ideas for basic research and clinical application.

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“…The top three bacteria association with CRC has also been previously reported. For instance, several studies suggested that Bacteroides are the bacteria that induce CRC [29,30] . Tingting, et al reported that Escherichia/Shigella were present at higher concentrations in the stool of CRC patients that in stools of healthy volunteers [31] .…”

Section: Discussionmentioning

confidence: 99%

Gut Microbes and Microbial Metabolites in Colorectal Cancer Complicated with Different Serum Albumin Levels

Han

et al. 2020

Preprint

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Background: Patients with colorectal cancer (CRC) are at risk of malnutrition. Gut microbes and microbial metabolites are involved in the initiation and development of CRC.Purpose:　To investigate serum protein levels in CRC patients and explore the role of gut microbes and microbial metabolites in CRCs complicated with different serum albumin levels.Methods: Overall, 398 CRC patients and same number of healthy volunteers in Huzhou Central Hospital from January 2016 to December 2018 were recruited to compare serum protein levels. The serological indicators were detected by Abbott Automatic Biochemical Analyzer(HCHL-YQ-SH-01). A total of 30 and 56 stool samples from CRC patients were used to detect intestinal microbes and microbial metabolites, respectively. Bacterial 16S V3-V4 and fungal ITS ribosomal DNA genes were sequenced and gas chromatography/mass spectrometry (GC/MS) was performed to detect microbial metabolites. Results: Some serum protein-related indicators in the CRC group were lower than those in the control group (p＜0.05). The total protein and albumin levels in colon cancer patients were lower than those in rectal cancer patients (p＜0.05). The higher abundance of Sutterella is correlated with lower serum albumin level in CRCs. There were statistically significant differences in the abundance of fungi including Agaricomycetes， Simplicillium, Sclerotiniaceae, and Exophiala among patients with different serum albumin levels. Multiple gut bacteria and fungi are closely related to serum albumin levels.We found some characteristic microbial metabolites in CRCs complicated with different serum protein levels.Conclusions: The different serum albumin levels were associated with the gut microbes and microbial metabolites in CRCs. It may provide novel ideas for basic research and clinical application.

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The germ-free mice monocolonization with Bacteroides fragilis improves azoxymethane/dextran sulfate sodium induced colitis-associated colorectal cancer

Cited by 16 publications

References 37 publications

Toll-like receptor 4 prevents AOM/DSS-induced colitis-associated colorectal cancer in Bacteroides fragilis gnotobiotic mice

Toll-like receptor 4 prevents AOM/DSS-induced colitis-associated colorectal cancer in Bacteroides fragilis gnotobiotic mice

Gut Microbes and Microbial Metabolites in Colorectal Cancer Complicated with Different Serum Albumin Levels

Gut Microbes and Microbial Metabolites in Colorectal Cancer Complicated with Different Serum Albumin Levels

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