The giant African snail Achatina fulica as natural intermediate host of Angiostrongylus cantonensis in Pernambuco, northeast Brazil

Thiengo, Silvana Carvalho; Maldonado, Arnaldo; Mota, Eduardo; Torres, Eduardo José Lopes; Caldeira, Roberta Lima; Carvalho, Omar S.; Oliveira, Ana Paula Martins de; Simões, Raquel de Oliveira; Fernandez, Mônica Ammon; Lanfredi, R. M.

doi:10.1016/j.actatropica.2010.01.005

Cited by 105 publications

(106 citation statements)

References 16 publications

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“…The variation of this rate is broad and has been observed in several municipalities, such as São Gonçalo (35.4%) and Barra do Piraí (10.3%), both in the State of Rio de Janeiro and Joinville/SC (27.4%) 12 , China (13.4% and 28.4%) 40,41 , Pernambuco (42%) 11 , and Japan (52.79%) 42 . The climatic characteristics of Baixada Santista are appropriate for the development of A. fulica and A. cantonensis.…”

Section: Several Snails Play Roles As Intermediate Hosts Formentioning

confidence: 99%

“…These data are especially useful and can be used by public health authorities to establish policies related to surveillance and planning of preventive actions. Isolated cases of eosinophilic meningoencephalitis have recently been reported in Brazil 9,11,14,15,46,47 . Thus, it is plausible that A. cantonensis continues to spread to new regions, increasing the risk of eosinophilic meningoencephalitis in humans.…”

Section: Several Snails Play Roles As Intermediate Hosts Formentioning

confidence: 99%

“…A. cantonensis has been observed in several regions of the world [3][4][5][6][7] , and they were distributed from Eastern Asia to other continents by two main hosts: rats (definitive hosts) and Achatina fulica Bowdich, 1822 (one of the intermediate hosts), especially during the Second World War 8 . Several species of land and freshwater snails have also been found to be naturally infected with A. cantonensis [9][10][11][12][13][14] . In Brazil, the occurrence of A. cantonensis has been reported in all states except for Acre [9][10][11][12][13][14][15][16][17] .…”

Section: Introductionmentioning

confidence: 99%

“…Several species of land and freshwater snails have also been found to be naturally infected with A. cantonensis [9][10][11][12][13][14] . In Brazil, the occurrence of A. cantonensis has been reported in all states except for Acre [9][10][11][12][13][14][15][16][17] . Man, being an accidental host, acquires parasitosis when eating foods contaminated with stagethree larvae (L3), raw or undercooked mollusks, and paratenic hosts such as shrimp, frogs, fish, and flatworms 4,[18][19][20] , as well as crabs and lizards 21,22 .…”

Section: Introductionmentioning

confidence: 99%

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Prevalence and distribution of Angiostrongylus cantonensis (Nematoda, Angiostrongylidae) in Achatina fulica (Mollusca, Gastropoda) in Baixada Santista, São Paulo, Brazil

Guerino

Pecora

Miranda

et al. 2017

Rev. Soc. Bras. Med. Trop.

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Introduction: Angiostrongylus cantonensis causes eosinophilic meningoencephalitis in humans. Worldwide expansion of this nematode is linked to the dispersion of their hosts. This study aimed to determine the prevalence of A. cantonensis infection in Achatina fulica in the nine municipalities that make up Baixada Santista, São Paulo, Brazil. Methods: Angiostrongylus cantonensis larvae were analyzed using optical microscopy. We performed polymerase chain reaction and restriction fragment length polymorphism using restriction endonuclease ClaI, directed to the internal transcribed spacer region 2 of A. cantonensis larval DNA. Results: Of the 540 snails analyzed, 117 (21.7%) were infected by A. cantonensis. For morphological and morphometric analyses, 60 larvae were used. Second-stage larvae were, on average, 358.2µm long and 26.4µm wide, while third-stage larvae were, on average, 450µm long and 21.12µm wide. The tails of the larvae ended in a fine tip. Conclusions: All municipalities comprising Baixada Santista had A. fulica that were naturally infected with A. cantonensis. All of the observed characteristics were typical of the species.

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Section: Several Snails Play Roles As Intermediate Hosts Formentioning

confidence: 99%

Section: Several Snails Play Roles As Intermediate Hosts Formentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Prevalence and distribution of Angiostrongylus cantonensis (Nematoda, Angiostrongylidae) in Achatina fulica (Mollusca, Gastropoda) in Baixada Santista, São Paulo, Brazil

Guerino

Pecora

Miranda

et al. 2017

Rev. Soc. Bras. Med. Trop.

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“…En Brasil, también se ha establecido que es huésped intermediario de A. vasorum y se sugiere como huésped potencial de A. costaricensis (5)(6)(7)(8).…”

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Standardization of a multiplex real-time PCR test for the identification of Angiostrongylus cantonensis, A. costaricensis and A. vasorum

2018

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Introducción. En el mundo, las angiostrongilosis de mayor impacto en salud humana y animal son ocasionadas por Angiostrongylus cantonensis, A. costaricensis y A. vasorum. En las personas, las formas clínicas son la meningitis eosinofílica y la angiostrongilosis abdominal, y, en los mamíferos cánidos, el daño cardiopulmonar. Se las consideran enfermedades emergentes debido a la propagación mundial del caracol africano Lissachatina fulica, un huésped intermediario de los parásitos. Los escasos métodos de identificación de Angiostrongylus spp. no son muy específicos ni sensibles y son costosos. Se necesita urgentemente una herramienta diagnóstica asequible, sensible y específicapara el manejo de las angiostrongilosis humana y la animal.Objetivo. Desarrollar una prueba de PCR múltiple en tiempo real (qPCR) para identificar las tres especies patógenas de Angiostrongylus.Materiales y métodos. Mediante un análisis bioinformático se seleccionó una secuencia del genoma ITS-2 de Angiostrongylus para garantizar la especificidad del cebador y las sondas. El ADN de los parásitos adultos (control positivo) y de las larvas se extrajo con el estuche DNeasyBlood & Tissue®.Las reacciones de la PCR cuantitativa se ejecutaron en un termociclador Smartcycler Cepheid®, usando el estuche de mezcla maestra QuantiTect®. Como control negativo, se utilizó ADN humano, de otros parásitos y del caracol africano.Resultados. Los valores del ciclo umbral para los controles positivos de ADN fueron: 21 para Angiostrongylus cantonensis, 22 para A. costaricensis y 31 para A. vasorum. En los controles negativos, el ciclo umbral fue cero. La qPCR mostró una eficiencia de amplificación de 2 (100 %).Conclusiones. En el laboratorio se estandarizó una qPCR múltiple para tres especies clínicamente significativas de Angiostrongylus.

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Angiostrongylus spp. of Public Health Importance

Silva

Mathison²

2018

Foodborne Parasites

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The giant African snail Achatina fulica as natural intermediate host of Angiostrongylus cantonensis in Pernambuco, northeast Brazil

Cited by 105 publications

References 16 publications

Prevalence and distribution of Angiostrongylus cantonensis (Nematoda, Angiostrongylidae) in Achatina fulica (Mollusca, Gastropoda) in Baixada Santista, São Paulo, Brazil

Prevalence and distribution of Angiostrongylus cantonensis (Nematoda, Angiostrongylidae) in Achatina fulica (Mollusca, Gastropoda) in Baixada Santista, São Paulo, Brazil

Standardization of a multiplex real-time PCR test for the identification of Angiostrongylus cantonensis, A. costaricensis and A. vasorum

Angiostrongylus spp. of Public Health Importance

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