The gingival tissues: the architecture of periodontal protection

Schroeder, Hubert E.; Listgarten, Max A.

doi:10.1111/j.1600-0757.1997.tb00097.x

Cited by 271 publications

(259 citation statements)

References 145 publications

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“…It consists of three structurally different sections, the junctional, sulcular, and oral gingival epithelium (Schroeder and Listgarten 1997). The junctional epithelium has a nonkeratinized structure with high turnover rate and limited differentiation.…”

Section: Introductionmentioning

confidence: 99%

Construction and characterization of a multilayered gingival keratinocyte culture model: the TURK-U model

Gürsoy

Könönen

et al. 2016

Cytotechnology

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In construction of epithelial cells as multilayers, the cells are grown submerged to confluence on fibroblast-embedded collagen gels and, then, lifted to air to promote their stratification. We recently demonstrated that gingival epithelial cells form uniform monolayers on semi-permeable nitrocellulose membranes, supported with a semi-solid growth medium, which allows the cells to grow at an airliquid-solid interface from the beginning of the culturing protocol. In this study, the aim was to further develop our previous model to form a multilayered gingival epithelial culture model. Two different epithelial cell lines (HaCaT from skin and HMK from gingiva) were used in all experiments. Both cell lines were grown first as monolayers for 3 days. After that, keratinocytes were trypsinized, counted and seeded on a sterile semi-permeable nitrocellulose membrane placed on the top of a semi-solid growth medium, forming an air-liquid-solid interface for the cells to grow. At days 1, 4, and 7, epithelial cells were fixed, embedded in paraffin, and sectioned for routine Hematoxylin-Eosin staining and immunohistochemistry for cytokeratin (Ck). At day 1, HMK cells grew as monolayers, while HaCaT cells stratified forming an epithelium with two to three layers. At day 4, a stratified epithelium in the HMK model had four to five layers and its proliferation continued up to day 7. HaCaT cells formed a dense and weakly proliferating epithelium with three to four layers of stratification at day 4 but the proliferation disappeared at day 7. At all days, both models were strongly positive for Ck5, Ck7, and Ck 19, and weakly positive for Ck10. Gingival epithelial cells stratify successfully on semi-permeable nitrocellulose membranes, supported with a semi-solid growth medium. This technique allows researchers to construct uniform gingival epithelial cell multilayers at an air-liquid-solid interface, without using collagen gels, resulting in a more reproducible method.

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Section: Introductionmentioning

confidence: 99%

Construction and characterization of a multilayered gingival keratinocyte culture model: the TURK-U model

Gürsoy

Könönen

et al. 2016

Cytotechnology

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“…At the same time gingival epithelium provides the protective integument of the periodontium. The histological appearance of human gingival epithelium has been studied in great detail and described by numerous authors [9][10][11][12][13]. This study report characterizes the epithelial cell behavior collected from clinically healthy and inflamed human gingival tissues.…”

Section: Discussionmentioning

confidence: 99%

Expression of PCNA in oral gingival epithelium of aggressive and, chronic periodontitis—A pilot study

Jyostna¹,

Reddy²,

Manyam³

et al. 2013

OJST

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Periodontal disease leads to loss of tissue homeostasis of tooth supporting structures. The mechanism of fibrous tissue degradation by various enzymes in chronic periodontitis and aggressive periodontitis was well documented but there is paucity of information regarding epithelial response. The aim of the present study was to evaluation of epithelial cell proliferation in Chronic Periodontitis, Aggressive Periodontitis and normal patients using Proliferating Cell Nuclear Antigen (PCNA). The study consists of 30 individuals who attended the Periodontia clinic of Vishnu Dental College. They were divided equally into 3 groups: chronic periodontitis (10) aggressive periodontitis (10) and normal individuals requiring gingival excision for crown lengthening (10). Excised gingival tissues were routinely processed and subjected to Immunohistochemical evaluation using Proliferating Cell Nuclear Antigen (PCNA). The mean proliferation index was almost equal in Aggressive periodontitis patients (36.55) and normal individuals (37.02) but it was high in Aggressive Periodontitis patients when compared to chronic periodontitis group (18.86). Observations of this study indicate the possibility of subclinical inflammation in clinically normal gingiva and difference in the types of microorganism associated with chronic periodontitis and aggressive periodontitis.

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“…A tissue surrounding tooth, known as periodontium, is characterized by four types of tissue: periodontal ligament, cementum, alveolar bone, and gingiva (Schroeder, 1986). These components play critical roles in the support, maintenance, and repair of the tooth and the supporting tissue under both physiological and pathological conditions.…”

Section: Topological Features Of Peri-implant Mucosamentioning

confidence: 99%

Biological Sealing and Defense Mechanisms in Peri-Implant Mucosa of Dental Implants

Yamaza¹,

Kido²

2011

Implant Dentistry - The Most Promising Discipline of Dentistry

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The gingival tissues: the architecture of periodontal protection

Cited by 271 publications

References 145 publications

Construction and characterization of a multilayered gingival keratinocyte culture model: the TURK-U model

Construction and characterization of a multilayered gingival keratinocyte culture model: the TURK-U model

Expression of PCNA in oral gingival epithelium of aggressive and, chronic periodontitis—A pilot study

Biological Sealing and Defense Mechanisms in Peri-Implant Mucosa of Dental Implants

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