The Gly152Val mutation possibly confers resistance to beta-lactam antibiotics in ovine &lt;i&gt;Staphylococcus aureus&lt;/i&gt; isolates

Sarhan, Sarhan R.; Hashim, Hayder O.; Al-Shuhaib, Mohammed Baqur S.

doi:10.4314/ovj.v9i4.12

Cited by 8 publications

(3 citation statements)

References 36 publications

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“…S. aureus was isolated phenotypically from the clinical samples using a previously reported method [ 32 , 33 ]. Furthermore, the molecular detection of S. aureus was performed by PCR amplification of the nuc gene with Fnuc and Rnuc primers ( Table 3 ) [ 34 ].…”

Section: Methodsmentioning

confidence: 99%

Mutation-Based Antibiotic Resistance Mechanism in Methicillin-Resistant Staphylococcus aureus Clinical Isolates

et al. 2021

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β-Lactam antibiotics target penicillin-binding proteins and inhibit the synthesis of peptidoglycan, a crucial step in cell wall biosynthesis. Staphylococcus aureus acquires resistance against β-lactam antibiotics by producing a penicillin-binding protein 2a (PBP2a), encoded by the mecA gene. PBP2a participates in peptidoglycan biosynthesis and exhibits a poor affinity towards β-lactam antibiotics. The current study was performed to determine the diversity and the role of missense mutations of PBP2a in the antibiotic resistance mechanism. The methicillin-resistant Staphylococcus aureus (MRSA) isolates from clinical samples were identified using phenotypic and genotypic techniques. The highest frequency (60%, 18 out of 30) of MRSA was observed in wound specimens. Sequence variation analysis of the mecA gene showed four amino acid substitutions (i.e., E239K, E239R, G246E, and E447K). The E239R mutation was found to be novel. The protein-ligand docking results showed that the E239R mutation in the allosteric site of PBP2a induces conformational changes in the active site and, thus, hinders its interaction with cefoxitin. Therefore, the present report indicates that mutation in the allosteric site of PBP2a provides a more closed active site conformation than wide-type PBP2a and then causes the high-level resistance to cefoxitin.

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Section: Methodsmentioning

confidence: 99%

Mutation-Based Antibiotic Resistance Mechanism in Methicillin-Resistant Staphylococcus aureus Clinical Isolates

et al. 2021

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“…A specific comprehensive tree was constructed according to the neighbour-joining protocol described by Sarhan et al. [ 23 ], with some modifications. The observed variants were compared with the deposited reference sequences using the NCBI-BLAST server.…”

Section: Methodsmentioning

confidence: 99%

Molecular detection and phylogenetic analysis of Vibrio cholerae genotypes in Hillah, Iraq

Al-Sa’ady

Baqer²,

Al-Salim³

2020

New Microbes and New Infections

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Vibrio cholerae is a cause of serious endemic diarrhoea associated with cholera in many regions in the world. A total of 256 stool and rectal swabs were collected from patients suspected to have cholera admitted to three hospitals in Hillah, Babylon Governorate, Iraq, for the period 1 September to 29 December 2017. After the routine culture of samples for isolation and identification of V. cholerae isolates, PCR was performed for molecular detection of V. cholerae isolates based on 16S ribosomal RNA gene. Toxigenicity was detected by RTX toxin genes. PCR technique emphasized molecular detection of V. cholerae for eight isolates. Only two isolates (25%) possessed both the rtxA and rtxC genes, while only three isolates (37.5%) possessed the rtxB gene. DNA sequencing was performed for the eight isolates via analysis and phylogenetic tree. The observed bacterial variants were compared to their neighbour homologous reference sequences using the National Center for Biotechnology Information (NCBI) BLAST server (Basic Local Alignment Search Tool; https://blast.ncbi.nlm.nih.gov/Blast.cgi ). The findings indicated that the eight investigated isolates of V. cholerae were positioned in three different phylogenetic positions. Partial sequence dissimilarities were reported between GenBank isolate accession number MK212155.1 and these six clustered GenBank accession numbers of the same species. For the first time in Babylon Governorate, Iraq, the molecular assay, sequencing and phylogenetic tree are reported for V. cholerae and their toxins isolated during the 2017 cholera outbreak.

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“…6.12.01 was utilized to group the haplotype of the variations identified into specific Hap categories (20). A phylogenetic tree was constructed according to the Neighbor-joining method (21).…”

Section: Phylogenetic Analysismentioning

confidence: 99%

A Survey of HBV Core/Pre-Core Mutations in Iraqi Patients with Chronic Hepatitis

Kadhim Jwaziri,

Esghaei,

Karbalaie Niya

et al. 2023

Hepat Mon

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Objectives: The present study was conducted to assess the pattern of HBV Core/Pre-Core mutations and HBV genotype in Iraqi patients with chronic hepatitis B virus (HBV) infection. Methods: In the current cross-sectional study in an Iraqi province, we evaluated 134 patients diagnosed with HBV hepatitis. We used PCR and, subsequently, Sanger sequencing to assess HBV Core/Pre-Core mutations. Sanger sequencing reads were further used for phylogenetic analysis and multiple sequence alignment. A phylogenetic tree was generated according to the neighbor-joining method. Results: The current study revealed that 58 (45%) of the patients were male, and 72 (55%) of them were female. The mean age of the patients was 36 ± 12.7 years, and the mean duration of infection was 5.2 ± 4.8 years. The results revealed 21 nucleic acid alterations in the samples analyzed. The generated phylogenetic tree divided samples into two genotypes. Pre-core/Core mutations were significantly associated with the treatment received (P = 0.0.001) but not with laboratory parameters. Most samples were matched with the genotype D clade, while only four samples were positioned adjacent to the genotype E clade. Direct nucleic acid translation disclosed five nucleic acid variants (73T>G, 347T>G, 364A>G, 365T>C, and 366A>G) on the core protein. Conclusions: This study has detected 21 nucleotide variants and 5 amino acid alterations within the coding sequences of the C gene. This study revealed that genotype D represents the primary genotype for the identified viral infections. The current study highlights the importance of these mutations evaluation for future, more comprehensive studies.

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The Gly152Val mutation possibly confers resistance to beta-lactam antibiotics in ovine <i>Staphylococcus aureus</i> isolates

Cited by 8 publications

References 36 publications

Mutation-Based Antibiotic Resistance Mechanism in Methicillin-Resistant Staphylococcus aureus Clinical Isolates

Mutation-Based Antibiotic Resistance Mechanism in Methicillin-Resistant Staphylococcus aureus Clinical Isolates

Molecular detection and phylogenetic analysis of Vibrio cholerae genotypes in Hillah, Iraq

A Survey of HBV Core/Pre-Core Mutations in Iraqi Patients with Chronic Hepatitis

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