The grape VvMBF1 gene improves drought stress tolerance in transgenic Arabidopsis thaliana

Yan, Qin; Hou, Hongmin; Singer, Stacy D.; Yan, Xiaoxiao; Guo, Rongrong

doi:10.1007/s11240-014-0508-2

Cited by 37 publications

(19 citation statements)

References 57 publications

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“…Moreover, the DNAbinding domain of MBF1c had a dominant-negative effect on heat tolerance when constitutively expressed in plants. Yan et al (2014) isolated a full-length MBF1 cDNA sequence (VvMBF1) from V. vinifera 3 Vitis amurensis that was up-regulated in the leaves of grape plants following both drought and abscisic acid treatments. Carbonell-Bejerano et al (2013) reported that berry MBF1a and MBF1c were up-regulated under high temperature when grape 'Muscat Hamburg' plants growing from cuttings were exposed to two different environmental regimes, 20°C/15°C (day/night) and 30°C/25°C (day/night), at the onset of ripening (veraison).…”

Section: Some Important Transcript Factors Could Be Involved In Heat mentioning

confidence: 99%

Integrating Omics and Alternative Splicing Reveals Insights into Grape Response to High Temperature

et al. 2017

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Heat stress is one of the primary abiotic stresses that limit crop production. Grape (Vitis vinifera) is a cultivated fruit with high economic value throughout the world, with its growth and development often influenced by high temperature. Alternative splicing (AS) is a widespread phenomenon increasing transcriptome and proteome diversity. We conducted high-temperature treatments (35°C, 40°C, and 45°C) on grapevines and assessed transcriptomic (especially AS) and proteomic changes in leaves. We found that nearly 70% of the genes were alternatively spliced under high temperature. Intron retention (IR), exon skipping, and alternative donor/acceptor sites were markedly induced under different high temperatures. Among all differential AS events, IR was the most abundant up-and down-regulated event. Moreover, the occurrence frequency of IR events at 40°C and 45°C was far higher than at 35°C. These results indicated that AS, especially IR, is an important posttranscriptional regulatory event during grape leaf responses to high temperature. Proteomic analysis showed that protein levels of the RNA-binding proteins SR45, SR30, and SR34 and the nuclear ribonucleic protein U1A gradually rose as ambient temperature increased, which revealed a reason why AS events occurred more frequently under high temperature. After integrating transcriptomic and proteomic data, we found that heat shock proteins and some important transcription factors such as MULTIPROTEIN BRIDGING FACTOR1c and HEAT SHOCK TRANSCRIPTION FACTOR A2 were involved mainly in heat tolerance in grape through up-regulating transcriptional (especially modulated by AS) and translational levels. To our knowledge, these results provide the first evidence for grape leaf responses to high temperature at simultaneous transcriptional, posttranscriptional, and translational levels.Heat stress is one of the main abiotic stresses limiting crop production worldwide. Global warming is predicted to be accompanied by more frequent and powerful extreme temperature events (Lobell et al., 2008). Therefore, a better understanding of the response of plants to heat stress is essential for improving their heat tolerance. In general, heat stress is a complex function of intensity (temperature in degrees), duration, and rate of increase in temperature. At very high temperatures, a catastrophic collapse of cellular organization may occur within minutes, which results in severe cellular injury and even cell death (Wahid et al., 2007). At moderately high temperatures, direct injuries include protein denaturation and aggregation and the increased fluidity of membrane lipids. Indirect or slower heat injuries include inactivation of enzymes, inhibition of protein synthesis, protein degradation, and loss of membrane integrity. These injuries eventually lead to a decline of net photosynthetic rate, reduced ion flux, production of toxic compounds and reactive oxygen species, and inhibition of growth. Actually, plants are not passively damaged by heat stress but respond to temperature changes by r...

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Section: Some Important Transcript Factors Could Be Involved In Heat mentioning

confidence: 99%

Integrating Omics and Alternative Splicing Reveals Insights into Grape Response to High Temperature

et al. 2017

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“…Water loss from leaves is strongly influenced by stomatal regulation, which is in turn affected by the concentration of ABA in the leaves ( Yan et al, 2014 ). The largest water loss was observed in WT, whereas 35S:: VlWRKY3 transgenic plants exhibited lower water loss ( Figure 3C ).…”

Section: Resultsmentioning

confidence: 99%

The Grape VlWRKY3 Gene Promotes Abiotic and Biotic Stress Tolerance in Transgenic Arabidopsis thaliana

et al. 2018

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WRKY transcription factors are known to play important roles in plant responses to various abiotic and biotic stresses. The grape WRKY gene, WRKY3 was previously reported to respond to salt and drought stress, as well as methyl jasmonate and ethylene treatments in Vitis labrusca × V. vinifera cv. ‘Kyoho.’ In the current study, WRKY3 from the ‘Kyoho’ grape cultivar was constitutively expressed in Arabidopsis thaliana under control of the cauliflower mosaic virus 35S promoter. The 35S::VlWRKY3 transgenic A. thaliana plants showed improved salt and drought stress tolerance during the germination, seedling and the mature plant stages. Various physiological traits related to abiotic stress responses were evaluated to gain further insight into the role of VlWRKY3, and it was found that abiotic stress caused less damage to the transgenic seedlings than to the wild-type (WT) plants. VlWRKY3 over-expression also resulted in altered expression levels of abiotic stress-responsive genes. Moreover, the 35S::VlWRKY3 transgenic A. thaliana lines showed improved resistance to Golovinomyces cichoracearum, but increased susceptibility to Botrytis cinerea, compared with the WT plants. Collectively, these results indicate that VlWRKY3 plays important roles in responses to both abiotic and biotic stress, and modification of its expression may represent a strategy to enhance stress tolerance in crops.

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“…Water loss from plants largely depends on stomatal regulation, which is in turn affected by ABA (Yan et al 2014;Guo et al 2014). Since the water loss rate was lower in the leaves from the transgenic seedlings than in the leaves from the WT plants at each time point (Fig.…”

Section: Identification Of Transgenic a Thaliana Linesmentioning

confidence: 99%

Expression of a grape bZIP transcription factor, VqbZIP39, in transgenic Arabidopsis thaliana confers tolerance of multiple abiotic stresses

Wang

Huang

et al. 2016

Plant Cell Tiss Organ Cult

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The basic region/leucine zipper (bZIP) transcription factors are known to play key roles in response to abiotic stress. In this study, a bZIP gene (VqbZIP39) was isolated from grape (Vitis quinquangularis) and constitutively expressed in Arabidopsis under control of the cauliflower mosaic virus 35S promoter. The transgenic Arabidopsis thaliana plants showed enhance salt and drought stress tolerance during seed germination and in the seedling and mature plant stages. Various physiological parameters related to stress responses were analyzed to gain further insight into the role of VqbZIP39 and it was found that osmotic stress caused less damage to the transgenic seedlings than to the corresponding wild type plants. This correlated with an increase in endogenous ABA content as a consequence of the constitutive overexpression of VqbZIP39, and the up-regulated expression of stress-inducible target genes associated with tolerance of drought, high-salt, and oxidative stresses. Our results suggest that the expression of VqbZIP39 in A. thaliana likely enhances the tolerance to multiple abiotic stresses through the ABA signaling pathway, and may therefore have a similar function in the response to abiotic stresses in grape.

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The grape VvMBF1 gene improves drought stress tolerance in transgenic Arabidopsis thaliana

Cited by 37 publications

References 57 publications

Integrating Omics and Alternative Splicing Reveals Insights into Grape Response to High Temperature

Integrating Omics and Alternative Splicing Reveals Insights into Grape Response to High Temperature

The Grape VlWRKY3 Gene Promotes Abiotic and Biotic Stress Tolerance in Transgenic Arabidopsis thaliana

Expression of a grape bZIP transcription factor, VqbZIP39, in transgenic Arabidopsis thaliana confers tolerance of multiple abiotic stresses

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