The Haemonetics(R) Cell Saver 5 washing properties: effect of different washing pump and centrifuge speeds

Abstract

This study evaluated the effect of different washing and centrifuge rates of the Cell Saver 5 on the quality of processed autologous blood. Autologous blood was washed with 1000 ml of sterile normal saline at centrifuge speed of 5650 revolutions per minute (rpm) (group I) or 4350 rpm (group II) with different washing pump speeds--500, 800 and 1000 ml/min. Hemoglobin, free hemoglobin, hematocrit, erythrocytes, leukocytes, platelets, and protein were measured before and after processing. The highest values of hemoglobin, hematocrit and erythrocytes were achieved using 800 and 1000 ml/min pump speeds in group I and 500 ml/min speed in group II. Red blood cells concentration was higher in group I. There were no significant changes of free hemoglobin removal within group I. In group II the lowest free hemoglobin was achieved when 1000 ml/min rate was used. Platelets and protein did not depend on wash pump speeds in both groups. Platelet recovery in group I was higher than in group II at all washing pump speeds. Leukocytes were not adequately removed at all pump speeds. The Cell Saver 5 produces optimum results when the high wash pump speeds (800 and 1000 ml/min) and standard centrifuge speed are used.

Impact of intraoperatively salvaged and washed blood on stimulated cytokine release in vitro

“…[32][33][34][35] In our investigation, we found an increase in the concentration of the WBCs in the salvaged blood compared to venous blood that was even higher than the increase of the Hct. This was in contrast to other investigations, 36,37 because usually during cell washing some WBCs are lost. However, the observed results in the concentration of WBCs and RBCs in our study might depend on the used continuous autotransfusion system (C.A.T.S., Fresenius).…”

“…This is in line with the findings of ten Brinke and colleagues 36 who reported that blood processing with an automatic autotransfusion system significantly enhances the PLT removal. In contrast, Naumenko and coworkers 37 observed a significant increase of the PLTs due to the processing. One reason for these inconsistent results might be differences in the counting of the flow cytometers used.…”

A quality assurance programme for cell salvage in cardiac surgery

“…Naumenko et al. showed that standard centrifuge speeds (5650 rpm) do not lead to greater mechanical damage to red blood cells than low centrifuge speeds (4350 rpm) [12]. Indeed, we showed higher free haemoglobin concentrations associated with the BRAT 2 cell saver.…”

“…The Cobe BRAT 2 Autologous Blood Recovery Unit (Sorin Biomedica, Milan, Italy) was used between October 2000 and May 2006, and the Haemonetics Cell Saver 5 (Haemonetics Corp., Braintree, MA, USA) between June 2006 and December 2007. These are both discontinuous cell washing autotransfusion systems, and use the same principle of centrifugal force to separate red cells from plasma; the BRAT 2 uses a 250‐ml Baylor bowl which is spun at 4400 revolutions per minute (rpm) and the Cell Saver 5 a 225‐ml Latham bowl at 5650 rpm [12]. Burman et al.…”

Pretransfusion Comparison of Dialyser-Based Hemoconcentrator with Cell Saver System for Perioperative Cell Salvage

“…17 Apart from being nephrotoxic, free hemoglobin has also been implicated in acute lung injury as well as endothelial and platelet aggregation dysfunction. 4 Studies have shown that there is no minimum amount of free hemoglobin that is safe for transfusion because even 100-mg/dL free hemoglobin has been shown to inhibit nitric oxideYdependent vasodilatation, leading to systemic and pulmonary artery hypertension. 6 However, Martin and Popovsky in their study suggested that the damage associated with free hemoglobin is directly related to the total amount of free hemoglobin infused rather than its concentration.…”