The head and neck cancer (HN-5) cell line properties extraction by AFM

Korayem, M. H.; Heidary, K.; Rastegar, Z.

doi:10.1186/s13036-020-00233-6

Cited by 10 publications

(5 citation statements)

References 23 publications

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“…33 for cancer diagnosis, such as head and neck cancer or ovarian cancer. 34 However, most studies only focus on the changes in the mechanical properties of single cell, but ignore the changes in that of tissues. In this study, fresh live surgical prostate specimens were collected and found that PCa tissues were softer than normal tissues, which exhibited its clinical significance.…”

Section: Discussionmentioning

confidence: 99%

“…AFM has been widely used to measure the deformability of single cell after the first detection in bladder cancer cell line since 1999 33 . Changes in the mechanical properties of single cell have been identified as one of the indicators for cancer diagnosis, such as head and neck cancer or ovarian cancer 34 . However, most studies only focus on the changes in the mechanical properties of single cell, but ignore the changes in that of tissues.…”

Section: Discussionmentioning

confidence: 99%

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Nanomechanical‐based classification of prostate tumor using atomic force microscopy

Zeng,

Zhang,

et al. 2023

The Prostate

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BackgroundThe loss of mechanical homeostasis between tumor cells and microenvironment is an important factor in tumor metastasis. In the process, mechanical forces affect cell proliferation, differentiation, migration and tissue development.AimsUsing high spatial resolution of Atomic force microscopy (AFM) technology, our study provides the direct measurement of the nanomechanical properties of prostate cancer clinical tissue specimens.Materials and MethodsAFM was used to determine the biomechanical properties of prostate tissue with different grade scores. K‐means clustering method and fuzzy C‐means were used to distinguish the cellular component in prostate tissue from non‐cellular component based on their viscoelasticity. Futhermore, AFM measurements in vitro cells, including metastatic prostate cells (PC‐3) and normal human prostate cells (PZ‐HPV‐7) were carried out.ResultsThe Young's modulus was decreased in prostate cancer progression, and the elasticity of cellular component in prostate cancer tissue was smaller than that of normal prostate tissue. PC‐3 cells were softer than PZ‐HPV‐7 cells. Further mechanism investigation showed that the difference in modulus between cancerous and normal prostate tissue may be associated with a greater actin cytoskeleton distribution inside the cancer cells.ConclusionThe results suggests that the nanomechanical properties can classify the prostate tumor, which could be used as an index for the identification and classification of cancer at cellular level.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Nanomechanical‐based classification of prostate tumor using atomic force microscopy

Zeng,

Zhang,

et al. 2023

The Prostate

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“…Korayem et al [ 276 ] recently used the microcantilever-based atomic force microscope to obtain various mechanical and physical properties of the head and neck cancer cells. These properties include the modulus of elasticity, cell topography, and viscoelastic properties.…”

Section: Microcantilever Sensors In Afm Applicationsmentioning

confidence: 99%

Review: Cantilever-Based Sensors for High Speed Atomic Force Microscopy

Alunda¹,

Lee

2020

Sensors

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This review critically summarizes the recent advances of the microcantilever-based force sensors for atomic force microscope (AFM) applications. They are one the most common mechanical spring–mass systems and are extremely sensitive to changes in the resonant frequency, thus finding numerous applications especially for molecular sensing. Specifically, we comment on the latest progress in research on the deflection detection systems, fabrication, coating and functionalization of the microcantilevers and their application as bio- and chemical sensors. A trend on the recent breakthroughs on the study of biological samples using high-speed atomic force microscope is also reported in this review.

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“…Biological atomic force microscopy (Bio-AFM) is a powerful tool, particularly at microscale- and nanoscale levels ( 15 ). It is widely used in biomedical research to determine the mechanical properties of various tumor tissues including in breast cancer ( 16 ), liver cancer ( 17 ), lung cancer ( 18 ), bladder cancer ( 19 ), colon cancer ( 20 ), head and neck cancer ( 21 ), and prostate cancer ( 22 ). It is used to explore the intrinsic relationship between tumor cell proliferation, adhesion, metastasis, and the mechanical properties of tumor cells.…”

Section: Introductionmentioning

confidence: 99%

Tissue mechanics modulate PCNP expression in oral squamous cell carcinomas with different differentiation

Zhang

Guo²,

Shen³

et al. 2023

Front. Oncol.

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BackgroundPEST-containing nuclear protein (PCNP), a novel zinc finger protein, participates in cell cycle regulation. Previous studies have confirmed that PCNP plays a role in mediating cellular development and invasion in a variety of cancer types. However, the relationship between PCNP expression and the occurrence and development of oral squamous cell carcinoma (OSCC) requires further exploration. In this study, we used biological atomic force microscopy to examine the histomorphological and mechanical properties of OSCC to explore the relationship between PCNP expression and differentiation of OSCC.MethodsSeventy-seven OSCC samples with varying degrees of differentiation were selected for hematoxylin and eosin staining, immunohistochemistry, and cellular mechanical measurement. The expression of PCNP and the mechanical properties such as stiffness and roughness of the tissue interface in OSCC samples were investigated. The Kaplan-Meier survival curve was utilized to assess the relationship of PCNP expression with patient survival.ResultsThe level of PCNP was significantly higher in well-differentiated OSCC than in moderately and poorly differentiated OSCC (P < 0.001). High expression of PCNP was specifically associated with higher tumor differentiation, lack of lymph node metastasis, and lower tumor node metastasis stage (all P < 0.05). Patients with high PCNP expression had a higher survival rate than those with low PCNP expression. The average variation of stiffness within a single tissue ranged from 347 kPa to 539 kPa. The mean surface roughness of highly, moderately, and poorly differentiated OSCC and paraneoplastic tissues were 795.53 ± 47.2 nm, 598.37 ± 45.76 nm, 410.16 ± 38.44 nm, and 1010.94 ± 119.07 nm, respectively. Pearson correlation coefficient demonstrated a positive correlation between PCNP expression and tissue stiffness of OSCC (R = 0.86, P < 0.001).ConclusionThe expression of PCNP was positively correlated with patient survival, tumor differentiation, and mechanical properties of tissue interfaces. PCNP is a potential biomarker for the early diagnosis and staging of OSCC. Furthermore, determination of the mechanical properties of the tissue interface could provide further useful information required for the detection and differentiation of OSCC.

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The head and neck cancer (HN-5) cell line properties extraction by AFM

Cited by 10 publications

References 23 publications

Nanomechanical‐based classification of prostate tumor using atomic force microscopy

Nanomechanical‐based classification of prostate tumor using atomic force microscopy

Review: Cantilever-Based Sensors for High Speed Atomic Force Microscopy

Tissue mechanics modulate PCNP expression in oral squamous cell carcinomas with different differentiation

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