The Highest Dosage Combination Activity Screening from the Leaf Fraction of <i>Melastoma malabathricum</i> with Antibiotic Gentamicin and Ciprofloxacin

Sari, Rafika; Pratiwi, Liza; Apridamayanti, Pratiwi

doi:10.3831/kpi.2022.25.2.101

Cited by 1 publication

(3 citation statements)

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“…This study reinforced the use of plants containing gallic acid in formulation development, specifically focusing Melastoma malabathricum [13]. Sari et al, 2022 showed the synergistic effects of EAF and Gentamicin combination, which acted as an effective antibiotic against Bacillus cereus, Escherichia coli, and Staphylococcus aureus. Therefore, this current study aims to develop and validate an analytical method for quantifying gallic acid levels within SNEDDS formulation, a combination of Melastoma malabathricum leaves EAF and Gentamicin, which is promising for the effective treatment of chronic wounds such as DFU.…”

Section: Introductionmentioning

confidence: 52%

“…Hainil et al, 2023 detected that EAF showed an inhibitory effect of 92%, compared to the 96% observed in ascorbic acid. In a separate study by Apridamayanti et al, 2022, the preparation of nanocream containing EAF indicated an antioxidant capacity of 1444.157±15.538 μg/ml as measured by the FRAP method [5,8,9].…”

Section: Introductionmentioning

confidence: 95%

“…Gallic acid can be naturally obtained from plants such as Allan blackia floribunda, Garcinia densivenia, Caesalpinia sappan, Diospyros cinnabarina, Paratecoma peroba, Psidium guajava, Phyllanthus emblica, Terminalia bellirica, Toona sinensis, Oenothera bienni, Rubus suavissimus, Punica granatum, T. bellerica, and Melastoma malabathricum [3][4][5][6][7]. Apridamayanti et al, 2022 conducted fractionation of Melastoma malabathricum leaves and reported gallic acid content of 49.29±0.49 mg/gram when using ethyl acetate as the solvent. Furthermore, the resulting ethyl acetate fraction (EAF) exhibited antioxidant activity against DPPH free radicals, with a value of 1.9±0.12 μg/ml, identical to the ascorbic acid activity of 1.9±0.2 μg/ml.…”

Section: Introductionmentioning

confidence: 99%

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The Development and Validation of Analytical Method for Evaluating Gallic Acid in Ethyl Acetate Fraction (Eaf) of Snedds Formulation: Quantitative Analysis With in Vitro Assay

APRIDAMAYANTI,

PRATIWI,

SARI

2024

Int J App Pharm

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Objective: This study aimed to develop a simple, accurate, precise, sensitive, robust, and stable analytical method for the evaluation of gallic acid in Self-Nanoemulsifying Drug Delivery System (SNEDDS) incorporating ethyl acetate fraction (EAF) of Melastoma malabathricum leaves in combination with Gentamicin. Methods: Validation process followed ICH guidelines and applied a reverse phase HPLC method with a mobile phase of acetonitrile-phosphoric buffer at pH 3.03 (20:80 v/v). The stationary phase consisted of a VP-ODS shim-pack C-18 column (250x4.6 mm) with a flow rate of 0.2 ml/min and detection at 263 nm using an Ultraviolet detector. Additionally, antioxidant activity was assessed through the DPPH and FRAP methods, and SPF value was determined with a UV/Vis spectrophotometer in the 290-390 nm wavelength range. Results: The results showed that the retention time of quercetin was 16.648 min with a tailing factor of 1.623. The regression equation (y=224689x-989000) had a concentration range of 10-55 µg/ml and a correlation value of 0.9920. Limit of Detection (LOD) and Limit of Quantification (LOQ) were found to be 2.394±0.086 and 7.254±0.260 µg/ml, respectively. Method accuracy, determined by recovery values at concentrations of 50%, 100%, and 150%, ranged from 91.18% to 109.49%. Repeatability inter-day variations were expressed as %RSD values of 1.027-1.963% for AUC and 0.150-0.145 for RT. Moreover, the applied method showed stability within a temperature range of 14 °C–35 °C. Analysis showed gallic acid content of 1.773±0.049 mg/g in SNEDDS EAF formulation. Antioxidant activity measured through the DPPH and FRAP methods yielded IC50 values of 4.167±0.552 µg/ml and 20.253±0.619 µg/ml, respectively, while SPF value at SNEDDS concentration of 150 µg/ml was 36.993±0.183. Conclusion: This study successfully developed a precise, accurate, specific, and stable method for quantifying gallic acid levels in SNEDDS EAF of Melastoma malabathricum leaves in combination with Gentamicin. Therefore, SNEDDS EAF formulation exhibited an effective wound-healing potential, supported by a robust quality control process.

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Section: Introductionmentioning

confidence: 52%