The highly sensitive detection of catecholamines using a microfluidic device integrated with an enzyme-modified pre-reactor for interferent elimination and an interdigitated array electrode

Quantitative Analysis of Epinephrine in Human Plasma Samples Using Kinetic Fluorometric Method Combined with Second-order Calibration

“…[2][3][4][5][6][7][8][9][10][11][12][13][14] These mainly include high-performance liquid chromatography (HPLC) with electrochemical detection 2 or mass spectrometry, 3 flow injection analysis (FIA) techniques, 4 electrochemical detection with various modified electrodes, 5,6 capillary electrophoresis (CE), 7 chemiluminescence (CL), 8 spectrophotometry, 9,10 and fluorescence detection. [11][12][13][14] However, most of these methods usually involve careful pretreatment steps when analyzing biological matrices.…”

Fabrication of 3D Interdigitated Array Electrode Consisting of Au Nanoparticles and Its Application for Biosensing

“…16 However, the process is time-consuming and requires two step of deposition for Ni and Au. Figure 3 shows cyclic voltammograms of the 3D-IDA and 2D-IDA electrodes in 0.5 M Na 2 SO 4 solution including 10 mM K 3 [Fe(CN) 6 ]. In this case, electrode length was narrowed to be 300 µm by pattering a photoresist on the electrodes, and Pt counter electrode was used.…”

Microfluidic enzymatic biosensing systems: A review

“…oxidation of H 2 O 2 when using oxidases) and the widespread availability of dedicated and affordable laboratory instruments. Detection limits typically are in the low mM range, however measurements down to a few pM are possible with microelectrodes (Hayashi et al, 2005). Amperometry requires a three electrode setup in which the functionalized working electrode (WE) is held at a defined potential relative to a reference electrode (RE) which typically is an Ag/AgCl electrode (Ronkainen et al, 2010).…”

“…This approach is well suited for first experiments since neither a sophisticated microfluidic design nor complicated fabrication steps are necessary. To increase the surface of the functionalized part and thereby the accessible enzymes, structures like pillars can be incorporated into the microfluidic design (Hayashi et al, 2005). In the course of functionalization, also an antifouling coating can be applied to the microfluidics (Picher et al, 2013).…”