The Hippo pathway effector Taz is required for cell morphogenesis and fertilization in zebrafish

Dingare, Chaitanya; Niedzwetzki, Alina; Klemmt, Petra; Godbersen, Svenja; Fuentes, Ricardo; Mullins, Mary C.; Lecaudey, Virginie

doi:10.1242/dev.167023

Cited by 24 publications

(21 citation statements)

References 62 publications

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“…Ccnb1 is expressed in 48 organs, including nervous system; oocyte; pectoral fin; trunk mesenchyme; and unfertilized egg, with highest expression level in blastula and third highest expression in the mature ovarian follicle [bgee.org—gene: ccnb1—ENSDARG00000051923— Danio rerio (zebrafish)]. Ccb1 is found in oocytes and needed for oocytes maturation and female sexual development (Kondo et al 1997 , 2001 ; Knoll-Gellida et al 2006 ; Wang et al 2007 ; Nagahama and Yamashita 2008 ; Yasuda et al 2013 ; Kotani et al 2013 ; Takahashi et al 2014 ; Horie and Kotani 2016 ; Dingare et al 2018 ; Takei et al 2018 ; Yi et al 2019 ). Ccnb1 is expressed in early zebrafish development (Yasuda et al 2010 ; Horie and Kotani 2016 ).…”

Section: Discussionmentioning

confidence: 99%

“…org-gene: ccnb1-ENSDARG00000051923-Danio rerio (zebrafish)]. Ccb1 is found in oocytes and needed for oocytes maturation and female sexual development (Kondo et al 1997(Kondo et al , 2001Knoll-Gellida et al 2006;Wang et al 2007;Nagahama and Yamashita 2008;Yasuda et al 2013;Kotani et al 2013;Takahashi et al 2014;Horie and Kotani 2016;Dingare et al 2018;Takei et al 2018;Yi et al 2019). Ccnb1 is expressed in early zebrafish development (Yasuda et al 2010;Horie and Kotani 2016).…”

Section: Ccnb1mentioning

confidence: 99%

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Shedding new light on early sex determination in zebrafish

2020

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In contrast to established zebrafish gene annotations, the question of sex determination has still not been conclusively clarified for developing zebrafish, Danio rerio, larvae, 28 dpf or earlier. Recent studies indicate polygenic sex determination (PSD), with the genes being distributed throughout the genome. Early genetic markers of sex in zebrafish help unravel co-founding sex-related differences to apply to human health and environmental toxicity studies. A qPCR-based method was developed for six genes: cytochrome P450, family 17, subfamily A, polypeptide 1 (cyp17a1); cytochrome P450, family 19, subfamily A, polypeptide 1a (cyp19a1a); cytochrome P450, family 19, subfamily A, polypeptides 1b (cyp19a1b); vitellogenin 1 (vtg1); nuclear receptor subfamily 0, group B, member 1 (nr0b1), sry (sex-determining region Y)-box 9b (sox9b) and actin, beta 1 (actb1), the reference gene. Sry-box 9a (Sox9a), insulin-like growth factor 3 (igf3) and double sex and mab-3 related transcription factor 1 (dmrt1), which are also known to be associated with sex determination, were used in gene expression tests. Additionally, Next-Generation-Sequencing (NGS) sequenced the genome of two adult female and male and two juveniles. PCR analysis of adult zebrafish revealed sex-specific expression of cyp17a1, cyp19a1a, vtg1, igf3 and dmrt1, the first four strongly expressed in female zebrafish and the last one highly expressed in male conspecifics. From NGS, nine female and four male-fated genes were selected as novel for assessing zebrafish sex, 28 dpf. Differences in transcriptomes allowed allocation of sex-specific genes also expressed in juvenile zebrafish.

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Section: Discussionmentioning

confidence: 99%

Section: Ccnb1mentioning

confidence: 99%

Shedding new light on early sex determination in zebrafish

2020

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“…The Tg[HuC:GFP] line labels early differentiated neurons (Park et al, 2000). The mutant lines yap fu48 and wwtr1 fu55 were generated using TALEN-induced mutagenesis strategy (Dingare et al, 2018).…”

Section: Fish Samplesmentioning

confidence: 99%

Yap/Taz-TEAD activity links mechanical cues to progenitor cell behavior during zebrafish hindbrain segmentation

et al. 2019

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Cells perceive their microenvironment through chemical and physical cues. However, how the mechanical signals are interpreted during embryonic tissue deformation to result in specific cell behaviors is largely unknown. The Yap/Taz family of transcriptional co-activators has emerged as an important regulator of tissue growth and regeneration, responding to physical cues from the extracellular matrix, and to cell shape and actomyosin cytoskeletal changes. In this study, we demonstrate the role of Yap/Taz-TEAD activity as a sensor of mechanical signals in the regulation of the progenitor behavior of boundary cells during zebrafish hindbrain compartmentalization. Monitoring of in vivo Yap/Taz activity during hindbrain segmentation indicated that boundary cells responded to mechanical cues in a cellautonomous manner through Yap/Taz-TEAD activity. Cell-lineage analysis revealed that Yap/Taz-TEAD boundary cells decreased their proliferative activity when Yap/Taz-TEAD activity ceased, which preceded changes in their cell fate from proliferating progenitors to differentiated neurons. Functional experiments demonstrated the pivotal role of Yap/Taz-TEAD signaling in maintaining progenitor features in the hindbrain boundary cell population.

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“…IVF was performed as in 100 . Briefly, sperm was collected from anesthetized heterozygous or wt males into Hank's solution and stored on ice until eggs are collected.…”

Section: In Vitro Fertilization (Ivf)mentioning

confidence: 99%

Biomechanical control of meiotic chromosomal bouquet and germ cell morphogenesis by the zygotene cilium

Mytils

Kumar

Qin³

et al. 2021

Preprint

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Meiosis is a cellular program essential for the production of haploid gametes. A hallmark of meiosis is chromosomal pairing via synaptonemal complexes, and a major focus traditionally has been to understand synaptonemal complex formation. However, chromosomal pairing also depends on cytoplasmic counterparts that tether and rotate telomeres on the nuclear envelope, shuffling chromosomes and mechanically driving their homology searches. Rotating telomeres slide on perinuclear microtubules and are ultimately pulled towards the centrosome, forming a configuration called the zygotene chromosomal bouquet. The bouquet is universally conserved and is essential for pairing and fertility. However, despite its discovery in 1900, how the cytoplasmic counterparts of bouquet formation are mechanically regulated has remained enigmatic. Here, by studying zebrafish oogenesis, we report and comprehensively characterize a previously unrecognized cilium in oocytes, which we term the zygotene cilium. We show that the zygotene cilium specifically connects to the bouquet centrosome and constitutes a cable system of the cytoplasmic bouquet machinery. Farther, zygotene cilia extend throughout the germline cyst, a conserved cellular organization of germ cells. By analyzing multiple ciliary mutants, we demonstrate that the zygotene cilium is essential for chromosomal pairing, germ cell morphogenesis, ovarian development and fertility. We further show that the zygotene cilium is conserved in both male meiosis in zebrafish, as well as in mammalian oogenesis. Our work uncovers the novel concept of a cilium as a critical player in meiosis and sheds new light on reproduction phenotypes in ciliopathies. Furthermore, most cells in metazoans are ciliated and exhibit specific nuclear dynamics. We propose a cellular paradigm that cilia can control chromosomal dynamics.

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The Hippo pathway effector Taz is required for cell morphogenesis and fertilization in zebrafish

Cited by 24 publications

References 62 publications

Shedding new light on early sex determination in zebrafish

Shedding new light on early sex determination in zebrafish

Yap/Taz-TEAD activity links mechanical cues to progenitor cell behavior during zebrafish hindbrain segmentation

Biomechanical control of meiotic chromosomal bouquet and germ cell morphogenesis by the zygotene cilium

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