The histone deacetylase inhibitor scriptaid enhances nascent mRNA production and rescues full-term development in cloned inbred mice

Thuan, Nguyen Van; Bui, Hong-Thuy; Kim, Jin‐Hoi; Hikichi, Takafusa; Wakayama, Sayaka; Kishigami, Satoshi; Mizutani, Eiji; Wakayama, Teruhiko

doi:10.1530/rep-08-0299

Cited by 141 publications

(124 citation statements)

References 26 publications

Supporting

Mentioning

118

Contrasting

Unclassified

Order By: Relevance

“…As Lys-5 is the last lysine to be acetylated, acetylated H4K5 reflects hyperacetylated histone H4, and this is correlated with a transcriptionally permissive state of the chromatin (26) that may be a necessary step for the embryonic genome activation occurring at the twocell stage. Indeed, a recent study by Van Thuan et al (27) has shown that Scriptaid, another inhibitor of HDAC, is able to increase the rate of de novo RNA synthesis in SCNT embryos at the two-cell stage. Comparison of the transcriptome profile at one-and two-cell stages in mouse SCNT and fertilized embryos revealed that a large subset of genes is indeed abnormally expressed during the embryonic genome activation (27).…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, a recent study by Van Thuan et al (27) has shown that Scriptaid, another inhibitor of HDAC, is able to increase the rate of de novo RNA synthesis in SCNT embryos at the two-cell stage. Comparison of the transcriptome profile at one-and two-cell stages in mouse SCNT and fertilized embryos revealed that a large subset of genes is indeed abnormally expressed during the embryonic genome activation (27). Among them, many transcription factors were found to be either up-regulated or repressed, therefore, affecting the reprogramming of the somatic genome into a totipotent one.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Somatic Nucleus Reprogramming Is Significantly Improved by m-Carboxycinnamic Acid Bishydroxamide, a Histone Deacetylase Inhibitor

Dai

Hao

Hou

et al. 2010

Journal of Biological Chemistry

View full text Add to dashboard Cite

Somatic cell nuclear transfer (SCNT) has shown tremendous potential for understanding the mechanisms of reprogramming and creating applications in the realms of agriculture, therapeutics, and regenerative medicine, although the efficiency of reprogramming is still low. Somatic nucleus reprogramming is triggered in the short time after transfer into recipient cytoplasm, and therefore, this period is regarded as a key stage for optimizing SCNT. Here we report that CBHA, a histone deacetylase inhibitor, modifies the acetylation status of somatic nuclei and increases the developmental potential of mouse cloned embryos to reach pre-and post-implantation stages. Furthermore, the cloned embryos treated by CBHA displayed higher efficiency in the derivation of nuclear transfer embryonic stem cell lines by promoting outgrowths. More importantly, CBHA increased blastocyst quality compared with trichostatin A, another prevalent histone deacetylase inhibitor reported previously. Use of CBHA should improve the productivity of SCNT for a variety of research and clinical applications, and comparisons of cells with different levels of pluripotency and treated with CBHA versus trichostatin A will facilitate studies of the mechanisms of reprogramming.Reprogramming of a terminally differentiated nucleus is successfully achieved in many species through somatic cell nuclear transfer (SCNT) 3 technology. However, its efficiency remains very low, limiting its application in agriculture to well bred livestock propagation and in species preservation (1) and regenerative medicine. The reprogramming process remains largely unknown; at the time of its transfer into an enucleated oocyte the somatic donor nucleus is in a configuration very different from a germ cell or an embryonic nucleus. Its own specific program of gene expression will have to be turned on, and the specific epigenetic modifications and chromatin configuration were erased (2). Extensive chromatin remodeling takes place as soon as the somatic nucleus is in contact with the oocyte cytoplasm, and two main types of epigenetic marks are directly involved in gene expression regulation; that is, methylation of histones or DNA and acetylation of histones (3-5). During normal pre-implantation development, the embryonic genome is progressively demethylated up to the early blastocyst stage. This is followed by differential remethylation in the two lineages of the blastocyst, the pluripotent inner cell mass and the trophoblast (6), but this process has been shown to be errorprone in SCNT embryos (7,8).Attempts to improve reprogramming during nuclear transfer have, therefore, focused primarily on modifying the epigenetic configuration of the donor nuclei before nuclear transfer. Therefore, treating donor cells with pharmacological agents to remove some epigenetic marks before NT may improve the ability of the donor cells to be fully reprogrammed by the recipient ooplasm. Pretreatment of bovine fibroblast donor cells by DNA demethylation agents such as 5-aza-2Ј-deoxycytidine or S-adenosy...

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Somatic Nucleus Reprogramming Is Significantly Improved by m-Carboxycinnamic Acid Bishydroxamide, a Histone Deacetylase Inhibitor

Dai

Hao

Hou

et al. 2010

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…These beneficial effects, corroborated in miniature pig SCNT embryos (Miyoshi et al 2010), have recently been questioned by Ono et al (2010), who compared the effects of different HDACis on cloning BD129F1 mice. The discrepancy of results in the mouse species can be probably attributed to strain-specific differences, as not all HDACis are equally effective in all genetic backgrounds (Kishigami et al 2007, Van Thuan et al 2009). With regard to the IE procedure, although Gasparrini et al (2003) previously reported the production of a cloned mouse using cytoplasts prepared by IE, we were unable to obtain live offspring from the IE group.…”

Section: Discussionmentioning

confidence: 99%

Effect of the enucleation procedure on the reprogramming potential and developmental capacity of mouse cloned embryos treated with valproic acid

Costa-Borges

González²,

Santaló³

et al. 2011

Reproduction

View full text Add to dashboard Cite

Mouse recipient cytoplasts for somatic cell nuclear transfer (SCNT) are routinely prepared by mechanical enucleation (ME), an invasive procedure that requires expensive equipment and considerable micromanipulation skills. Alternatively, oocytes can be enucleated using chemically assisted (AE) or chemically induced (IE) enucleation methods that are technically simple. In this study, we compared the reprogramming potential and developmental capacity of cloned embryos generated by ME, AE, and IE procedures and treated with the histone deacetylase inhibitor valproic acid. A rapid and almost complete deacetylation of histone H3 lysine 14 in the somatic nucleus followed by an equally rapid and complete re-acetylation after activation was observed after the injection of a cumulus cell nucleus into ME and AE cytoplasts. In contrast, histone deacetylation occurred at a much lower level in IE cytoplasts. Despite these differences, the cloned embryos generated from the three types of cytoplasts developed into blastocysts of equivalent total and inner cell mass mean cell numbers, and the rates of blastocyst formation and embryonic stem cell derivation were similar among the three groups. The cloned embryos produced from ME and AE cytoplasts showed an equivalent rate of full-term development, but no offspring could be obtained from the IE group, suggesting a lower reprogramming capacity of IE cytoplasts. Our results demonstrate the usefulness of AE in mouse SCNT procedures, as an alternative to ME. AE can facilitate oocyte enucleation and avoid the need for expensive microscope optics, or for potentially damaging Hoechst staining and u.v. irradiation, normally required in ME procedures.

show abstract

“…In fact, different types of epigenetic modifications can interact with each other (as shown in Figure 2); the abnormality in one type of epigenetic modification must result in the abnormality in other types of epigenetic modifications. For example, due to incomplete DNA demethylation, cloned embryos would exhibit lower level of histone acetylation and H3K4 methylation, and higher level of H3K9 methylation, that is why treatment of cloned embryos with histone deacetylase inhibitor (trichostatin A, sodium butyrate, Scriptaid, and VPA), as well as inducing express of H3K9me3 demethylases JMJD2B, could improve their developmental ability (Rybouchkin et al 2006;Van Thuan et al 2009;Das et al 2010;Xu et al 2012;Antony et al 2013). …”

Section: Main Barricades Of Reprogramming Somatic Cells By Oocytesmentioning

confidence: 99%

Somatic cell reprogrammed by oocyte: process and barricade

Wang

et al. 2014

Animal Cells and Systems

View full text Add to dashboard Cite

Somatic cell nuclear transfer (SCNT) is a technology in which a somatic nucleus is transferred into the cytoplasm of a matured oocyte -reconstructed embryos have the capacity to develop to term. Why somatic cells can be reprogrammed by oocytes -the answer for this question must exist in the cytoplasm of matured oocytes. In this review, totipotent characters of matured oocytes were discussed, which may confer matured oocytes with the capacity to reprogram somatic nucleus. Moreover, the procedure of SCNT also makes a possibility for somatic nucleus to be reprogrammed by oocytes. Compared with fertilized embryos, embryos derived from SCNT exhibit low developmental ability; the barricades in reprogramming process and their possible reasons were also discussed. This review maybe can benefit the mechanism research of SCNT technology and can make contribution for improving the efficiency of this technology.

show abstract

The histone deacetylase inhibitor scriptaid enhances nascent mRNA production and rescues full-term development in cloned inbred mice

Cited by 141 publications

References 26 publications

Somatic Nucleus Reprogramming Is Significantly Improved by m-Carboxycinnamic Acid Bishydroxamide, a Histone Deacetylase Inhibitor

Somatic Nucleus Reprogramming Is Significantly Improved by m-Carboxycinnamic Acid Bishydroxamide, a Histone Deacetylase Inhibitor

Effect of the enucleation procedure on the reprogramming potential and developmental capacity of mouse cloned embryos treated with valproic acid

Somatic cell reprogrammed by oocyte: process and barricade

Contact Info

Product

Resources

About