The histopathological and morphometric investigation of the effects of systemically administered humic acid on alveolar bone loss in ligature‐induced periodontitis in rats

Çalışır, Metin; Akpınar, Aysun; Poyraz, Ömer; Göze, Fahrettin; Çınar, Ziynet

doi:10.1111/jre.12329

Cited by 23 publications

(20 citation statements)

References 49 publications

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“…Humic acid was obtained from peat coming from the western Black Sea region and was diluted in sterile saline solution to reach the study concentrations (80 mg/kg) [ 20 ]. The concentration of the trace elements in the humic acid solution such as Si, Se, Ca, Mg, Fe, and Zn is provided in Table 1 .…”

Section: Methodsmentioning

confidence: 99%

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Humic Acid Enhances Wound Healing in the Rat Palate

Çalışır

Akpınar

Talmaç

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Introduction Humic acid was previously shown to enhance cutaneous wound healing and show antibacterial properties; however, it has not been used for wound healing in the oral cavity. Thus, the goal of this study was the evaluation of the effect of the humic acid on the healing of excisional wounds in an experimental rat study. Materials and Methods A circular wound on mid-palatal surfaces was made on a total of 77 Wistar rats by using a 3-mm biopsy punch under anesthesia. The animals were divided into 4 groups as baseline, saline control (0.09%), chlorhexidine gluconate (0.05%), and humic acid (80 mg/kg) and were treated with these materials for 7 days. Results The rats were observed for 3 weeks in order to track the wound closure rates. Both humic acid treatment and chlorhexidine gluconate treatment resulted in statistically significant enhanced rate of wound closure compared to the saline control on both the 1st and 2nd weeks of treatment. Humic acid treatment for the wounds in the palate resulted in enhanced recovery compared to not only saline control but also chlorhexidine gluconate treatment. Conclusion In this study, humic acid was shown to enhance healing of oral wounds for the first time in the literature. These findings indicate that humic acid can be used as an alternative to current treatment methods for oral wounds.

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Section: Methodsmentioning

confidence: 99%

“…In the oral cavity, we have previously shown that humic acid prevents alveolar bone loss and reduce inflammation in rats [ 20 ]. In addition, carbohydrate-derived fulvic acid, which is a major constituent of humic acids, has been shown to have a broad-spectrum antimicrobial activity against orally active microorganisms [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Humic Acid Enhances Wound Healing in the Rat Palate

Çalışır

Akpınar

Talmaç

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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“…Ionel and Lucaciu 2015, Çalışır et al, 2016 comes in aggrement with our result, they found that PD in rats after 4 weeks revealed pronounced inflammation of PDL and advanced resorption of the alveolar bone crest. [21][22] While after 4 weeks, the same group showed irregular areas of external root resorption with the destruction of periodontal ligament fibers and massive inflammation. hasan and palmer 2016 aggremented with our results.…”

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confidence: 99%

Protective Effect of Moringa Oleifera Extract on Experimentally LPS- Induced Periodontitis.

Alshwerf¹,

Amin²,

Ibrahim³

et al. 2017

IJAR

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Periodontal disease denotes a group of oral inflammatory infections. This infectious disease severity varies from minor and reversible gingiva inflammation (gingivitis) to chronic damage of CT. 1 This process causes separation of the gum tissues from the tooth, producing a periodontal pocket and bone loss, which causing tooth loosening. 2 Lipopolysaccharide (LPS) is a main compound present in the membrane of Gram negative bacteria. It has the ability to initiate immune responses by stimulating cells that reside in the periodontal tissues, leading to releasing of large numbers of inflammatory mediators including interleukins, chemokines and adhesion molecules. 3 After recognition and presentation of microbes to the appropriate cells, cytokines of the innate response, including tumor necrosis factor alpha (TNF-α), interleukin-1beta (IL-1β) and interleukin-6 (IL-6), are the first to appear in the periodontal disease pathogenesis pathways. 4 IL-1β and IL-6 are signature innate cytokines and have been characteristically associated with inflammatory cell migration and osteoclastogenesis. 5

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“…The ligature‐induced periodontitis model was used because it mimics features of human periodontitis, including the formation of an inflammatory infiltrate, loss of attachment, and loss of alveolar bone. The majority of studies have kept the ligature in place from 15 to 60 days for the induction of periodontal destruction (Çalışır, Akpınar, Poyraz, Göze, & Çınar, ; de Molon et al, ; Fontana, Kurachi, Mendonça, & Bagnato, ; Johnson, ). In this study, ligatures were removed 27 days after their placement.…”

Section: Discussionmentioning

confidence: 99%

Molecular analyses of two bacterial sampling methods in ligature‐induced periodontitis in rats

Fontana

Grecco

Bagnato

et al. 2018

Clinical & Exp Dental Res

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The prevalence profile of periodontal pathogens in dental plaque can vary as a function of the detection method; however, the sampling technique may also play a role in determining dental plaque microbial profiles. We sought to determine the bacterial composition comparing two sampling methods, one well stablished and a new one proposed here. In this study, a ligature‐induced periodontitis model was used in 30 rats. Twenty‐seven days later, ligatures were removed and microbiological samples were obtained directly from the ligatures as well as from the periodontal pockets using absorbent paper points. Microbial analysis was performed using DNA probes to a panel of 40 periodontal species in the checkerboard assay. The bacterial composition patterns were similar for both sampling methods. However, detection levels for all species were markedly higher for ligatures compared with paper points. Ligature samples provided more bacterial counts than paper points, suggesting that the technique for induction of periodontitis could also be applied for sampling in rats. Our findings may be helpful in designing studies of induced periodontal disease‐associated microbiota.

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The histopathological and morphometric investigation of the effects of systemically administered humic acid on alveolar bone loss in ligature‐induced periodontitis in rats

Abstract: Within the limits of this study, it can be suggested that humic acid, when administered systemically as an 80 mg/kg dose, may prevent alveolar bone loss and reduce inflammation in the rat model.

Cited by 23 publications

References 49 publications

Humic Acid Enhances Wound Healing in the Rat Palate

Humic Acid Enhances Wound Healing in the Rat Palate

Protective Effect of Moringa Oleifera Extract on Experimentally LPS- Induced Periodontitis.

Molecular analyses of two bacterial sampling methods in ligature‐induced periodontitis in rats

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