The honey bee tyramine receptor AmTYR1 and division of foraging labor

Scheiner, Ricarda; Kulikovskaja, Leonora; Thamm, Markus

doi:10.1242/jeb.098475

Cited by 15 publications

(23 citation statements)

References 11 publications

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“…Tyramine (TA) is a biological precursor of octopamine (OA), and both these biogenic amines cooperatively and also independently affect glycogenolysis, behavior, reproduction and stress resistance in various insect species (Candy, 1978;Downer, 1979;Mentel et al, 2003;Roeder, 2005). In honey bees, OA and TA levels in the brain appear to influence locomotory behavior (Fussnecker et al, 2006), gustatory perception (Scheiner et al, 2014a) and foraging preference (Scheiner et al, 2014b). We have recently uncovered associations between TYR1 expression in the fat body, ovariole number (Wang et al, 2012b), gustatory perception and foraging behavior (Y.W., G.V.A.…”

Section: Discussion Adaptive Responses To An Anticipatory Starvationmentioning

confidence: 99%

Starvation stress during larval development facilitates an adaptive response in adult worker honey bees (Apis mellifera L.)

Wang

Kaftanoğlu

Brent

et al. 2016

Journal of Experimental Biology

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Most organisms are constantly faced with environmental changes and stressors. In diverse organisms, there is an anticipatory mechanism during development that can program adult phenotypes. The adult phenotype would be adapted to the predicted environment that occurred during organism maturation. However, whether this anticipatory mechanism is present in eusocial species is questionable because eusocial organisms are largely shielded from exogenous conditions by their stable nest environment. In this study, we tested whether food deprivation during development of the honey bee (Apis mellifera), a eusocial insect model, can shift adult phenotypes to better cope with nutritional stress. After subjecting fifth instar worker larvae to short-term starvation, we measured nutrition-related morphology, starvation resistance, physiology, endocrinology and behavior in the adults. We found that the larval starvation caused adult honey bees to become more resilient toward starvation. Moreover, the adult bees were characterized by reduced ovary size, elevated glycogen stores and juvenile hormone (JH) titers, and decreased sugar sensitivity. These changes, in general, can help adult insects survive and reproduce in food-poor environments. Overall, we found for the first time support for an anticipatory mechanism in a eusocial species, the honey bee. Our results suggest that this mechanism may play a role in honey bee queen-worker differentiation and worker division of labor, both of which are related to the responses to nutritional stress.

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Section: Discussion Adaptive Responses To An Anticipatory Starvationmentioning

confidence: 99%

Starvation stress during larval development facilitates an adaptive response in adult worker honey bees (Apis mellifera L.)

Wang

Kaftanoğlu

Brent

et al. 2016

Journal of Experimental Biology

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“…For determining gustatory responsiveness, each bee was sequentially stimulated by presenting a series of sucrose concentrations (0.1, 0.3, 1, 3, 10 and 30% w/v) to her antennae (for details, see Scheiner et al, 2013Scheiner et al, , 2014a. The sum of proboscis extension responses (PER) to the stimulations with seven different sucrose concentrations constitutes the gustatory response score (GRS) of a bee, which is an excellent measure of its gustatory responsiveness (Scheiner et al, 2003(Scheiner et al, , 2014a.…”

Section: Gustatory Responsivenessmentioning

confidence: 99%

“…The sum of proboscis extension responses (PER) to the stimulations with seven different sucrose concentrations constitutes the gustatory response score (GRS) of a bee, which is an excellent measure of its gustatory responsiveness (Scheiner et al, 2003(Scheiner et al, , 2014a. To evaluate the effect of neuroactive substances, changes in GRS 30 min after application compared with GRS prior to treatment were calculated and compared between groups.…”

Section: Gustatory Responsivenessmentioning

confidence: 99%

Learning, gustatory responsiveness and tyramine differences across nurse and forager honeybees

Scheiner

Reim

Søvik

et al. 2017

Journal of Experimental Biology

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Honeybees are well known for their complex division of labor. Each bee sequentially performs a series of social tasks during its life. The changes in social task performance are linked to gross differences in behavior and physiology. We tested whether honeybees performing different social tasks (nursing versus foraging) would differ in their gustatory responsiveness and associative learning behavior in addition to their daily tasks in the colony. Further, we investigated the role of the biogenic amine tyramine and its receptors in the behavior of nurse bees and foragers. Tyramine is an important insect neurotransmitter, which has long been neglected in behavioral studies as it was believed to only act as the metabolic precursor of the better-known amine octopamine. With the increasing number of characterized tyramine receptors in diverse insects, we need to understand the functions of tyramine on its own account. Our findings suggest an important role for tyramine and its two receptors in regulating honeybee gustatory responsiveness, social organization and learning behavior. Foragers, which were more responsive to gustatory stimuli than nurse bees and performed better in appetitive learning, also differed from nurse bees in their tyramine brain titers and in the mRNA expression of a tyramine receptor in the brain. Pharmacological activation of tyramine receptors increased gustatory responsiveness of nurse bees and foragers and improved appetitive learning in nurse bees. These data suggest that a large part of the behavioral differences between honeybees may be directly linked to tyramine signaling in the brain.

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“…CA 5 calyx; vL 5 vertical lobe; gL 5 gamma lobe. [Color figure can be viewed at wileyonlinelibrary.com]Scheiner, Kulikovskaja, et al, 2014). There are two possible interpretations for these findings.…”

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confidence: 97%

“…It is conceivable that this change in gustatory responsiveness is mediated by AmTAR1, because the respective gene is a candidate gene in a quantitative trait locus correlating with gustatory responsiveness and different aspects of foraging behavior (Hunt et al, 2007). Furthermore, Amtar1 mRNA is expressed differentially in the gnathal ganglia of pollen and nectar foragers (Scheiner, Kulikovskaja, & Thamm, 2014). This finding is exciting, because nectar and pollen foragers also differ in their gustatory responsiveness (Scheiner, Erber, & Page, 1999;Scheiner, Page, & Erber, 2001), and gustatory stimuli are mediated by the gnathal ganglia (Rehder, 1989).…”

mentioning

confidence: 99%

Neuronal distribution of tyramine and the tyramine receptor AmTAR1 in the honeybee brain

Thamm

Scholl

Reim

et al. 2017

J of Comparative Neurology

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Tyramine is an important neurotransmitter, neuromodulator, and neurohormone in insects. In honeybees, it is assumed to have functions in modulating sensory responsiveness and controlling motor behavior. Tyramine can bind to two characterized receptors in honeybees, both of which are coupled to intracellular cAMP pathways. How tyramine acts on neuronal, cellular and circuit levels is unclear. We investigated the spatial brain expression of the tyramine receptor AmTAR1 using a specific antibody. This antibody detects a membrane protein of the expected molecular weight in western blot analysis. In honeybee brains, it labels different structures which process sensory information. Labeling along the antennal nerve, in projections of the dorsal lobe and in the gnathal ganglion suggest that tyramine receptors are involved in modulating gustatory and tactile perception. Furthermore, the ellipsoid body of the central complex and giant synapses in the lateral complex show AmTAR1-like immunoreactivity (AmTAR1-IR), suggesting a role of this receptor in modulating sky-compass information and/or higher sensor-motor control. Additionally, intense signals derive from the mushroom bodies, higher-order integration centers for olfactory, visual, gustatory and tactile information. To investigate whether AmTAR1-expressing brain structures are in vicinity to tyramine releasing sites, a specific tyramine antibody was applied. Tyramine-like labeling was observed in AmTAR1-IR positive structures, although it was sometimes weak and we did not always find a direct match of ligand and receptor. Moreover, tyramine-like immunoreactivity was also found in brain regions without AmTAR1-IR (optic lobes, antennal lobes), indicating that other tyramine-specific receptors may be expressed there.

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The honey bee tyramine receptor AmTYR1 and division of foraging labor

Cited by 15 publications

References 11 publications

Starvation stress during larval development facilitates an adaptive response in adult worker honey bees (Apis mellifera L.)

Starvation stress during larval development facilitates an adaptive response in adult worker honey bees (Apis mellifera L.)

Learning, gustatory responsiveness and tyramine differences across nurse and forager honeybees

Neuronal distribution of tyramine and the tyramine receptor AmTAR1 in the honeybee brain

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