The Hsp27 gene is not required for Drosophila development but its activity is associated with starvation resistance

Hao, Xiao‐Jiang; Zhang, Sen; Timakov, Benjamin; Zhang, Ping

doi:10.1379/csc-308.1

Cited by 46 publications

(26 citation statements)

References 50 publications

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“…Hsp27 is expressed highly in fly neuronal tissue where it increases resistance to oxidative stress (Liao et al, 2008). It is essential for development and specifically acts as a molecular chaperone to refold denatured proteins generated under starvation conditions (Hao et al, 2007). Transcription of the other stress protein, Hsp23 is regulated during development by binding of the ecdysone-receptor complex to the promoter region of the Hsp23 gene (Mestril et al, 1986).…”

Section: Resultsmentioning

confidence: 99%

A new approach for investigating venom function applied to venom calreticulin in a parasitoid wasp

Siebert

Wheeler

Werren

2015

Toxicon

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A new method is developed to investigate functions of venom components, using venom gene RNA interference knockdown in the venomous animal coupled with RNA sequencing in the envenomated host animal. The vRNAi/eRNA-Seq approach is applied to the venom calreticulin component (v-crc) of the parasitoid wasp Nasonia vitripennis. Parasitoids are common, venomous animals that inject venom proteins into host insects, where they modulate physiology and metabolism to produce a better food resource for the parasitoid larvae. vRNAi/eRNA-Seq indicates that v-crc acts to suppress expression of innate immune cell response, enhance expression of clotting genes in the host, and up-regulate cuticle genes. V-crc KD also results in an increased melanization reaction immediately following envenomation. We propose that v-crc inhibits innate immune response to parasitoid venom and reduces host bleeding during adult and larval parasitoid feeding. Experiments do not support the hypothesis that v-crc is required for the developmental arrest phenotype observed in envenomated hosts. We propose that an important role for some venom components is to reduce (modulate) the exaggerated effects of other venom components on target host gene expression, physiology, and survival, and term this venom mitigation. A model is developed that uses vRNAi/eRNA-Seq to quantify the contribution of individual venom components to total venom phenotypes, and to define different categories of mitigation by individual venoms on host gene expression. Mitigating functions likely contribute to the diversity of venom proteins in parasitoids and other venomous organisms.

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Section: Resultsmentioning

confidence: 99%

A new approach for investigating venom function applied to venom calreticulin in a parasitoid wasp

Siebert

Wheeler

Werren

2015

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“…Three day old flies (formulation fed and control) were starved in empty bottles containing filter paper strips soaked with water [61]. Mortality of starved flies was recorded every 12 hours.…”

Section: Methodsmentioning

confidence: 99%

In Vivo Effects Of Traditional Ayurvedic Formulations in Drosophila melanogaster Model Relate with Therapeutic Applications

et al. 2012

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BackgroundAyurveda represents the traditional medicine system of India. Since mechanistic details of therapy in terms of current biology are not available in Ayurvedic literature, modern scientific studies are necessary to understand its major concepts and procedures. It is necessary to examine effects of the whole Ayurvedic formulations rather than their “active” components as is done in most current studies.MethodsWe tested two different categories of formulations, a Rasayana (Amalaki Rasayana or AR, an herbal derivative) and a Bhasma (Rasa-Sindoor or RS, an organo-metallic derivative of mercury), for effects on longevity, development, fecundity, stress-tolerance, and heterogeneous nuclear ribonucleoprotein (hnRNP) levels of Drosophila melanogaster using at least 200 larvae or flies for each assay.ResultsA 0.5% (weight/volume) supplement of AR or RS affected life-history and other physiological traits in distinct ways. While the size of salivary glands, hnRNP levels in larval tissues, and thermotolerance of larvae/adult flies improved significantly following feeding either of the two formulations, the median life span and starvation resistance improved only with AR. Feeding on AR or RS supplemented food improved fecundity differently. Feeding of larvae and adults with AR increased the fecundity while the same with RS had opposite effect. On the contrary, feeding larvae on normal food and adults on AR supplement had no effect on fecundity but a comparable regime of feeding on RS-supplemented food improved fecundity. RS feeding did not cause heavy metal toxicity.ConclusionsThe present study with two Ayurvedic formulations reveals formulation-specific effects on several parameters of the fly's life, which seem to generally agree with their recommended human usages in Ayurvedic practices. Thus, Drosophila, with its very rich genetic tools and well-worked-out developmental pathways promises to be a very good model for examining the cellular and molecular bases of the effects of different Ayurvedic formulations.

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“…Together, these results suggest that Hsp27 may be required for differentiation of muscle cells. In contrast, Drosophila embryos lacking a homolog of human Hsp27 develop normally and, surprisingly, loss of Hsp27 does not alter the resistance of flies to heat shock or oxidative injury (Hao et al 2007). The Drosophila homolog of Hsp27 lacks phosphorylated serines conserved among vertebrate homologs of Hsp27 (Ingolia and Craig 1982) and displays a constitutive localization to the nucleus of cells under control conditions (Marin and Tanguay 1996) not typical of vertebrate Hsp27.…”

Section: Introductionmentioning

confidence: 99%

Hsp27 is persistently expressed in zebrafish skeletal and cardiac muscle tissues but dispensable for their morphogenesis

Tucker

Ustyugov

Bryantsev

et al. 2009

Cell Stress and Chaperones

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Constitutive expression of Hsp27 has been demonstrated in vertebrate embryos, especially in developing skeletal and cardiac muscle. Results of several previous studies have indicated that Hsp27 could play a role in the development of these tissues. For example, inhibition of Hsp27 expression has been reported to cause defective development of mammalian myoblasts in vitro and frog embryos in vivo. In contrast, transgenic mice lacking Hsp27 develop normally. Here, we examined the distribution of Hsp27 protein in developing and adult zebrafish and effects of suppressing Hsp27 expression using phosphorodiamidate morpholino oligonucleotides (PMO) on zebrafish development. Consistent with our previous analysis of hsp27 messenger RNA expression, we detected the protein Hsp27 in cardiac, smooth, and skeletal muscle of both embryonic and adult zebrafish. However, embryos lacking detectable Hsp27 after injection of antisense hsp27 PMO exhibited comparable heart beat rates to that of control embryos and cardiac morphology was indistinguishable in the presence or absence of Hsp27. Loss of Hsp27 also had no effect on the structure of the skeletal muscle myotomes in the developing embryo. Finally, embryos injected with antisense hsp27 and scrambled control PMO displayed equal motility. We conclude that Hsp27 is dispensable for zebrafish morphogenesis but could play a role in long-term maintenance of heart and muscle tissues.

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The Hsp27 gene is not required for Drosophila development but its activity is associated with starvation resistance

Cited by 46 publications

References 50 publications

A new approach for investigating venom function applied to venom calreticulin in a parasitoid wasp

A new approach for investigating venom function applied to venom calreticulin in a parasitoid wasp

In Vivo Effects Of Traditional Ayurvedic Formulations in Drosophila melanogaster Model Relate with Therapeutic Applications

Hsp27 is persistently expressed in zebrafish skeletal and cardiac muscle tissues but dispensable for their morphogenesis

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