The Human in Vitro Anti‐type 4 Pneumococcal Polysaccharide Antibody Response is Regulated by Suppressor T Cells

Griffioen, Arjan W.; Rijkers, Ger T.; Toebes, Elly A.H.; Zegers, B. J. M.

doi:10.1111/j.1365-3083.1991.tb01541.x

Cited by 22 publications

(11 citation statements)

References 25 publications

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“…They stated that "usually the effects produced by Ts and Ta are counterbalanced: this is why the Ab response to SSS-III is similar in magnitude in thymus-bearing and athymic mice and why Ts and Ta have escaped detection for so many years." The existence of stimulatory and inhibitory T cells has been confirmed by other groups (23,24) and, more recently, we further substantiated that CD4 ϩ T lymphocytes exert stimulatory, and CD8 ϩ T lymphocytes exert inhibitory, effects, and that these effects depended on the CD40L-CD40 interaction (9,10). In the present manuscript, we demonstrate that CD4 ϩ T cells expressing CD40L help the IgM Ab response to soluble pneumococcal caps-PS via an intermediate cell type.…”

Section: Discussionsupporting

confidence: 70%

CD4+ T Lymphocytes Expressing CD40 Ligand Help the IgM Antibody Response to Soluble Pneumococcal Polysaccharides via an Intermediate Cell Type

Jeurissen

Billiau²,

Moens³

et al. 2006

The Journal of Immunology

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Streptococcus pneumoniae causes serious infections in children, the elderly, and immunocompromised patients. Protection against infections with S. pneumoniae is mediated through Abs against the capsular polysaccharides (caps-PS). We previously showed that the murine Ab response to caps-PS is dependent on CD40-CD40L interaction. In the present paper, we addressed the question of whether the CD40-CD40L-mediated modulation of the anti-caps-PS immune reaction is the result of a direct interaction between B lymphocytes and T lymphocytes or of an indirect interaction. SCID/SCID mice reconstituted with B lymphocytes from wild-type mice did not mount anti-caps-PS Abs. SCID/SCID mice reconstituted with B lymphocytes from wild-type mice and CD4+ T lymphocytes from wild-type mice but not CD4+ T lymphocytes from CD40L knockout mice stimulated the anti-caps-PS Ab response. This indicated that CD4+ T lymphocytes stimulated the anti-caps-PS Ab response in a CD40L-dependent manner. SCID/SCID mice reconstituted with B lymphocytes from CD40 knockout mice and CD4+ T lymphocytes from wild-type mice generated an anti-caps-PS Ab response that could be inhibited by MR1, a blocking anti-CD40L Ab. These data indicated that CD4+ T lymphocytes stimulated the anti-caps-PS Ab response in an indirect way. Finally, lethally irradiated CD40 knockout mice reconstituted with bone marrow from wild-type mice mounted an anti-caps-PS Ab response that was comparable to the Ab response in wild-type mice, revealing that the required CD40 was on hemopoietic cells. In conclusion, we provide evidence that CD4+ T lymphocytes expressing CD40L stimulate the Ab response to soluble caps-PS by interacting with CD40-expressing non-B cells.

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Section: Discussionsupporting

confidence: 70%

CD4+ T Lymphocytes Expressing CD40 Ligand Help the IgM Antibody Response to Soluble Pneumococcal Polysaccharides via an Intermediate Cell Type

Jeurissen

Billiau²,

Moens³

et al. 2006

The Journal of Immunology

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“…Up to now, only scarce evidence for a possible role of T lymphocytes and the CD40-CD40L interaction in the human immune response to caps-PS is available. Griffioen et al [11,20] provided evidence that CD4 + T lymphocytes stimulate and CD8 + T lymphocytes inhibit the human immune response to caps-PS in vitro. Leiva et al [12] showed induction of CD40L on T lymphocytes by stimulation with TI-2 antigens, which suggested that the CD40-CD40L interaction could play a role in the human immune response to caps-PS.…”

Section: Discussionmentioning

confidence: 99%

The human antibody response to pneumococcal capsular polysaccharides is dependent on the CD40‐CD40 ligand interaction

et al. 2004

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Protection against infections with Streptococcus pneumoniae is mediated by antibodies against the capsular polysaccharides (caps-PS). Here we show that in in vitro experiments CD4 + T lymphocytes stimulate and CD8 + T lymphocytes inhibit the human anti-caps-PS antibody response. Using antagonistic anti-CD40 and antagonistic anti-CD40 ligand (CD40L) monoclonal antibodies, we showed that the CD4 + T lymphocyte-mediated stimulation is dependent on the CD40-CD40L interaction. The role of CD40L was further illustrated by the observation that CD4 + T lymphocytes obtained from a patient with hyper-IgM syndrome were unable to enhance the immune response to caps-PS. Furthermore, CD4 + T lymphocytes from cord blood, which did not express CD40L in response to stimulation with caps-PS, failed to stimulate the antibody response of adult B lymphocytes to caps-PS. These in vitro findings were confirmed by in vivo experiments in which SCID/SCID mice were reconstituted with human mononuclear cells. Furthermore, we showed that caps-PS induce production of IL-4, IL-6, IL-10, and IFN-+ , and that this enhanced production was inhibited by blocking the CD40-CD40L interaction. This is the first demonstration that the human immune response to caps-PS, which is markedly regulated by T lymphocytes, is dependent on the CD40-CD40L interaction.

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“…It has been suggested that typical TI-2 antigens can activate murine T cells in an antigen-specific manner and induce them to provide help to B cells for the production of IgG antibody responses (33). Griffioen et a1 (34) suggest that the ongoing antibody responses to some TI-2 antigens, such as pneumococcal capsular polysaccharide, are under the strong influence of regulatory T cells that can exert either an amplifying effect or a suppressive effect. This antibody response to TI-2 antigens is regulated by an antigenspecific suppressor function that is MHC restricted.…”

Section: Discussionmentioning

confidence: 99%

Serum antibodies to klebsiella capsular polysaccharides in ankylosing spondylitis

Sahly

Podschun

Sass

et al. 1994

Arthritis & Rheumatism

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Objective. To measure antibodies to Klebsiella capsular polysaccharides in the sera of HLA-B27 positive patients with ankylosing spondylitis (AS), compared with HLA-B27 positive and HLA-B27 negative healthy control subjects.Methods. Antibodies were detected by means of an enzyme-linked immunosorbent assay specific for each of the 77 known Klebsiella serotypes.Results. Significantly elevated frequencies and titers of antibodies to capsular polysaccharides K26, K36, and K50 were detected in sera from AS patients, compared with controls.Conclusion. These results suggest the predominance of Klebsiella serotypes K26, K36, and K50 in patients with AS.

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The Human in Vitro Anti‐type 4 Pneumococcal Polysaccharide Antibody Response is Regulated by Suppressor T Cells

Cited by 22 publications

References 25 publications

CD4+ T Lymphocytes Expressing CD40 Ligand Help the IgM Antibody Response to Soluble Pneumococcal Polysaccharides via an Intermediate Cell Type

CD4+ T Lymphocytes Expressing CD40 Ligand Help the IgM Antibody Response to Soluble Pneumococcal Polysaccharides via an Intermediate Cell Type

The human antibody response to pneumococcal capsular polysaccharides is dependent on the CD40‐CD40 ligand interaction

Serum antibodies to klebsiella capsular polysaccharides in ankylosing spondylitis

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