The human tuftelin gene: cloning and characterization

Mao, Zhengkuan; Shay, Boaz; Hekmati, M; Fermon, Eran; Taylor, Angie; Dafni, Leah; Heikinheimo, Kristiina; Lustmann, J.; Fisher, Larry W.; Young, Marian F.; Deutsch, D.

doi:10.1016/s0378-1119(01)00749-1

Cited by 40 publications

(57 citation statements)

References 18 publications

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“…In the present study, we examined some of the soft tissues that were previously indicated to express partial tuftelin mRNA sequences, using blast search of EST databases (human and mouse) (Mao et al, 2001). …”

Section: Results and Discussion Expression And Localization Of Tuftelmentioning

confidence: 99%

“…Briefly, the full mouse tuftelin sequence was RT-PCR-amplified using the following primers: DD112 (forward), 5 0 -CTCATACGAC-CTCCGCGTGAAGATG-3 0 , and DD108 (reverse), 5 0 -TCA-GGTTTCCACCACCCGGATGAG-3 0 (Mao et al, 2001), followed by cloning into pGEM-T Easy Vector (Promega, Madison, WI). The tuftelin sequence was amplified by PCR using SP6 and T7 primers.…”

Section: In Situ Hybridizationmentioning

confidence: 99%

“…Further search of the human and mouse expressed sequence tag (EST) databases (Mao et al, 2001;Deutsch et al, 2002) and Northern blot analysis (MacDougall et al, 1998) revealed that, in addition to tuftelin expression in the developing and mineralizing enamel, partial tuftelin cDNA sequences were also detected in many normal as well as cancerous soft tissues. Initial studies indicated that tuftelin protein is also synthesized in some of these tissues, such as eye, stomach, brain, and kidney (Mao et al, 2001;Deutsch et al, 2002).…”

mentioning

confidence: 99%

“…Initial studies indicated that tuftelin protein is also synthesized in some of these tissues, such as eye, stomach, brain, and kidney (Mao et al, 2001;Deutsch et al, 2002). However, the precise pattern of expression and localization to specific cell types within these tissues were not fully explored.…”

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confidence: 99%

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Localization, quantification, and characterization of tuftelin in soft tissues

Leiser

Blumenfeld

Haze

et al. 2007

The Anatomical Record

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Tuftelin was initially found in the developing and mature extracellular enamel. Here we describe our novel discovery of tuftelin cellular distribution (protein and mRNA) in six soft tissues. The expression levels of tuftelin mRNA were significantly higher in mouse kidney and testis, in which oxygen levels are hovering closely to hypoxia under normal conditions. Anat Rec, 290:449-454, 2007. 2007 Wiley-Liss, Inc.

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Section: Results and Discussion Expression And Localization Of Tuftelmentioning

confidence: 99%

Section: In Situ Hybridizationmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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Localization, quantification, and characterization of tuftelin in soft tissues

Leiser

Blumenfeld

Haze

et al. 2007

The Anatomical Record

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“…La expresión de esta proteína se ha observado en numerosos tipos de tejidos en diferentes orígenes embriológicos (mesenquimal, epitelial y neuronal) (Mao et al, 2001;Deutsch et al, 2002;Shay et al, 2009), tejidos mineralizados normales y con presencia de células cáncerosas (Mao et al, 2001;Deutsch et al, 2002;2011;Leiser et al, 2007). Su peso molecular es de 44 KDa con un punto isoeléctrico de 5,7 , y se considera una proteína no específica del esmalte (Deutsch et al, 2002).…”

Section: Introductionunclassified

Expresión de Tuftelina en Gérmenes Dentales Humanos

et al. 2017

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RESUMEN:La tuftelina es una proteína secretada en la matriz adamantina en desarrollo durante la formación del esmalte. Su función continúa sin esclarecerse, aunque se presume que juega un papel importante en la biomineralización de esmalte y dentina, así como en el desarrollo del órgano dental. Con el presente estudio se identificó su localización en las diferentes estructuras de gérmenes dentales de fetos humanos, conforme a los resultados se observó su expresión en el estadio pre-secretor observándose en el citoplasma de los ameloblastos, retículo estrellado, papila dental, así como en el estrato intermedio; en el secretor se identificó principalmente en la unión amelodentinaria, y en la superficie externa del esmalte, observando una marcada expresión de la proteína en la porción basal del proceso odontoblástico, pero no en la matriz extracelular de la dentina. De acuerdo a los resultados obtenidos se puede considerar que su expresión se presenta tanto en la amelogénesis, como en la odontogénesis en tejidos sin mineralizar, PALABRAS CLAVE: tuftelina; inmunohistoquímica; amelogénesis. INTRODUCCIÓNEl esmalte dental es el tejido más duro y mineralizado del cuerpo humano. Su formación se caracteriza por un proceso de desarrollo intenso y organizado (Fincham et al., 1999), que se forma químicamente por matriz inorgánica, (95 %), agua (3-5 %) y por matriz orgánica (1-2 %)

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Tuftelin‐derived peptide facilitates remineralization of initial enamel caries in vitro

et al. 2020

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With the gradual discovery of functional domains in natural proteins, several biologically inspired peptides have been designed for use as biomaterials for hard tissue regeneration and repair. In this study, we designed a tuftelin-derived peptide (TDP) and tested its effects on hydroxyapatite crystallization and remineralization of initial enamel carious lesions in vitro. Using circular dichroism spectroscopy, we found that TDP contained 36.1% β-sheets and β-turns, which could be influenced by calcium ions. We verified the ability of TDP to crystallize hydroxyapatite using transmission electron microscopy and its ability to bind to the enamel surface and hydroxyapatite using confocal laser scanning microscopy and Langmuir adsorption isotherms (K = 881.56, N = 1.41 × 10 −5). Artificial enamel lesions were generated on human enamel blocks and subjected to a 12-day pH cycling model and were treated with 25 μM TDP, 1 g/L sodium fluoride (NaF), or deionized water. We analyzed the results of remineralization by surface microhardness testing, polarized light microscopy, and transverse microradiography. The TDP group showed significantly higher surface microhardness recovery (49.21 ± 1.66%), shallower lesions (34.89 ± 4.05 μm), and less mineral loss (871.33 ± 81.49 vol%•μm) after pH cycling than the deionized water group (p < .05). There were no significant differences between the TDP and NaF groups. Our experiment indicated that TDP could regulate hydroxyapatite crystallization and promote remineralization of enamel caries in vitro.

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The human tuftelin gene: cloning and characterization

Cited by 40 publications

References 18 publications

Localization, quantification, and characterization of tuftelin in soft tissues

Localization, quantification, and characterization of tuftelin in soft tissues

Expresión de Tuftelina en Gérmenes Dentales Humanos

Tuftelin‐derived peptide facilitates remineralization of initial enamel caries in vitro

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