The Hypoosmotic Swelling Test: An Update

Jeyendran, Rajasingam S.; Ven, H. H. Van Der; Zaneveld, L. J. D.

doi:10.3109/01485019208987714

Cited by 175 publications

(79 citation statements)

References 40 publications

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“…Jeyendran et al (1992) found in dogs that the changes provoked by the hypoosmotic environment could be used for predicting sperm quality. Our findings also show the usability of this test for interpreting the membrane resistance of sperm.…”

Section: Discussionmentioning

confidence: 99%

“…• The speed of sperm movement was routinely determined using the propulsivity method according to Baker et al (1957 Jeyendran et al (1984Jeyendran et al ( , 1992) was used to determine the membrane integrity of the plasmatic membrane, the membrane on the tail and the consequent morphological changes in the tail for documenting live and dead sperm. The semen was incubated for 30 min in a solution of sodium citrate with 1.35% fructose at 150 mosmoles.…”

Section: Methodsmentioning

confidence: 99%

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Functional evaluation of dog ejaculates with priority given to the aspect of acrosome integrity

Vĕzník¹,

Švecová²,

Zajicova³

et al. 2003

Vet. Med. - Czech

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38 ejaculates of dogs of various breeds from 2 to 6 years old were examined in a short-term 120 min survival test. The examination focused on the functional parameters of sperm (motility, percentage of live spermatozoa, acrosomal integrity) and their morphology. The levels of relationship between individual criteria of sperm analysis were demonstrated. Statistically highly significant correlations between motility, ratio of live spermatozoa, percentage of intact acrosomes and percentage of spermatozoa with altered superficial structures were proven. Our findings proved the sensitivity of superficial structures, primarily plasmatic membranes, and the higher resistance of structures with an organelle basis. Strict morphological sperm analysis provides important information about the rise in qualitative alterations of ejaculates caused by changes in superficial structures during the course of a short-term survival test.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Functional evaluation of dog ejaculates with priority given to the aspect of acrosome integrity

Vĕzník¹,

Švecová²,

Zajicova³

et al. 2003

Vet. Med. - Czech

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“…The e ect could have been due to membrane damage as the treated spermatozoa failed to swell in hypoosmotic bu er. The swelling of the sperm tail in hypoosmotic bu er indicates that the sperm membrane is intact and that the sperm are functionally active [8,9]. In addition, Trypan blue staining showed that the treated sperm were not viable, suggesting that the Pongam ia oil also has spermicidal activity.…”

Section: Discussionmentioning

confidence: 99%

“…The e ect of oil on sperm membrane integrity was studied by the hypoosmotic swelling (HOS) test [8,9]. Following treatment with Pongam ia oil as described above, sperm were incubated at 37¯C for 30 min with 1 mL of hypoosmotic solution (7.35 g Na-citrate, 2H 2 O, and 13.51 g fructose in 1 L of H2O having 150 mosmol).…”

Section: At Erials and M Ethodsmentioning

confidence: 99%

Spermicidal Activity of Seed Oil of Pongamia Glabra

Bandivdekar

Moodbidri

2002

Archives of Andrology

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“…If no sperm was present in either sample men were advised to seek clinical care for azoospermia. Briefly, sperm viability was evaluated by the hypo-osmotic swelling (HOS) assay (Schrader et al, 1990;Jeyendran et al, 1992). The hyperactivated motility (HTM)-integrated visual optical system (IVOS) computer-aided semen analysis (CASA) platform with video playback (Hamilton Thorne Biosciences, Beverly, MA, USA) was employed to assess eight measures of motility in a 20-µm-depth micro chamber slide (Leja, Nieuw-Vennep, Netherlands).…”

Section: Semen Quality Assessmentmentioning

confidence: 99%

Male birthweight, semen quality and birth outcomes

et al. 2017

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STUDY QUESTION: What are the relations among birthweight (BW), semen parameters and birth outcomes in a population-based sample?SUMMARY ANSWER: BW is unrelated to semen parameters, which are in turn unrelated to birth outcomes. WHAT IS KNOWN ALREADY:In clinical settings, there has been suggestion that semen parameters are related to BW when comparing fertile and infertile men; however, findings have been less clear in more general populations.STUDY DESIGN, SIZE, DURATION: Questionnaire data and semen samples were collected at baseline from 427 male participants of the population-based Longitudinal Investigation of Fertility and the Environment (LIFE) prospective cohort study from 2005 to 2009, who were followed prospectively to assess pregnancy outcomes among 226 singleton births. PARTICIPANTS/MATERIALS, SETTING, METHODS:Men of at least 18 years of age who were married or in a committed relationship and trying to conceive were eligible for participation; physician-diagnosed infertility was an exclusion criterion. Participants were recruited from two geographic areas and semen samples were analyzed for 34 quality parameters categorized as general, motility, morphology, sperm head and sperm chromatin structure using methods including computer-aided semen analysis integrated visual optical system and sperm chromatin structure assay. Linear and mixed models were used for statistical analysis of the relations between men's BW, semen parameters, and BW, gestational age at delivery, birth length, head circumference and ponderal index of singleton births. MAIN RESULTS AND THE ROLE OF CHANCE:No association was observed between male BW and semen parameters or birth outcomes. Few associations were observed between semen parameters and birth outcomes, and the observed statistically significant associations were isolated and without a consistent pattern that would suggest an association between BW and birth outcomes.LIMITATIONS, REASONS FOR CAUTION: Men's BW was self-reported and may be subject to some imprecision. Semen analysis was performed the day after collection, an approach that impacts the assessment of motility and that may limit inference from our analyses of motility measures. In addition, inclusion criteria for selection into the cohort limits generalizability to generally healthy couples trying to conceive and without known subfertility. WIDER IMPLICATIONS OF THE FINDINGS:Despite suggestions from prior studies of male in utero exposures impacting BW and male reproductive health, there appears to be little support for such relations in this generally healthy population.

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The Hypoosmotic Swelling Test: An Update

Cited by 175 publications

References 40 publications

Functional evaluation of dog ejaculates with priority given to the aspect of acrosome integrity

Functional evaluation of dog ejaculates with priority given to the aspect of acrosome integrity

Spermicidal Activity of Seed Oil of Pongamia Glabra

Male birthweight, semen quality and birth outcomes

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