The <i>TRANSPARENT TESTA16</i> Locus Encodes the ARABIDOPSIS BSISTER MADS Domain Protein and Is Required for Proper Development and Pigmentation of the Seed Coat

Nési, Nathalie; Debeaujon, Isabelle; Jond, Clarisse; Stewart, Amanda; Jenkins, Gareth I.; Caboche, Michel; Lepiniec, Loïc

doi:10.1105/tpc.004127

Cited by 316 publications

(380 citation statements)

References 69 publications

(92 reference statements)

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“…PAs accumulate in the endothelium and chalaza of wild-type seeds and confer a dark-brown color to the seed coat after desiccation (Debeaujon et al, 2003). By contrast, tt16 seeds synthetize PAs only in the chalaza and micropyle region; thus, the rest of the seed coat appears mostly yellow (Nesi et al, 2002). All three complementation lines produced fully colored brown seeds, indicating that PA accumulation was restored in the tt16 mutant endothelium (Supplemental Figure 6).…”

Section: Tt16 Mediates the Crosstalk Between Endothelium And Nucellusmentioning

confidence: 87%

“…Dexamethasone (DEX) treatment releases the GR transcription factor chimeric protein from a cytoplasmic HEAT SHOCK PROTEIN90 complex that prevents its nuclear translocation and therefore its functionality (Schena et al, 1991). Addition of DEX to Pro35S:TT16-GR Arabidopsis plants caused phenotypic adaxialization of leaves and stunted growth identical to plants that overexpress TT16 (Supplemental Figure 7) (Nesi et al, 2002), indicating that the TT16-GR chimeric protein retains function. To prevent indirect transcriptional effects of the inducible TT16-GR protein, we infiltrated 35S:TT16-GR inflorescences with cycloheximide (CHX), an inhibitor of protein synthesis.…”

Section: Tt16 Represses Hva22d a Negative Regulator Of Autophagy Andmentioning

confidence: 99%

“…The kpl-1, tt16-2, tt16-3, ProFIS:FIS-GUS, and ProMEA:MEA-GUS lines are in the Ws-2 accession (Luo et al, 2000;Nesi et al, 2002;Ron et al, 2010). The agl62-2/+, mc1, lsd1, fie-12/+, msi1-1/+, fis2-5/+, goa-1, and ProFWA:FWA-GUS lines are in the Col-0 accession (Kinoshita et al, 2004;Coll et al, 2010;Prasad et al, 2010;Roszak and Köhler, 2011).…”

Section: Plant and Genetic Materialsmentioning

confidence: 99%

“…TT16 promotes the differentiation of the seed endothelium, the innermost layer of the seed coat. tt16 mutant seeds display abnormally shaped endothelium cells and fail to synthetize PAs (Nesi et al, 2002;Lepiniec et al, 2006). We analyzed the central longitudinal section of tt16 mutant ovules and seeds between 0 and 8 DAF ( Figures 1F to 1J).…”

Section: Tt16 Promotes Nucellus Degenerationmentioning

confidence: 99%

“…The agl62-2/+, mc1, lsd1, fie-12/+, msi1-1/+, fis2-5/+, goa-1, and ProFWA:FWA-GUS lines are in the Col-0 accession (Kinoshita et al, 2004;Coll et al, 2010;Prasad et al, 2010;Roszak and Köhler, 2011). The tt16-1 mutant was isolated in the Ws-2 accession and then backcrossed to the Col-0 accession more than three times (Nesi et al, 2002). The tt16-1;goa-1 double mutant is in the Col-0 accession (Prasad et al, 2010).…”

Section: Plant and Genetic Materialsmentioning

confidence: 99%

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Endosperm and Nucellus Develop Antagonistically in Arabidopsis Seeds

et al. 2016

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In angiosperms, seed architecture is shaped by the coordinated development of three genetically different components: embryo, endosperm, and maternal tissues. The relative contribution of these tissues to seed mass and nutrient storage varies considerably among species. The development of embryo, endosperm, or nucellus maternal tissue as primary storage compartments defines three main typologies of seed architecture. It is still debated whether the ancestral angiosperm seed accumulated nutrients in the endosperm or the nucellus. During evolution, plants shifted repeatedly between these two storage strategies through molecular mechanisms that are largely unknown. Here, we characterize the regulatory pathway underlying nucellus and endosperm tissue partitioning in Arabidopsis thaliana. We show that Polycomb-group proteins repress nucellus degeneration before fertilization. A signal initiated in the endosperm by the AGAMOUS-LIKE62 MADS box transcription factor relieves this Polycomb-mediated repression and therefore allows nucellus degeneration. Further downstream in the pathway, the TRANSPARENT TESTA16 (TT16) and GORDITA MADS box transcription factors promote nucellus degeneration. Moreover, we demonstrate that TT16 mediates the crosstalk between nucellus and seed coat maternal tissues. Finally, we characterize the nucellus cell death program and its feedback role in timing endosperm development. Altogether, our data reveal the antagonistic development of nucellus and endosperm, in coordination with seed coat differentiation.

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Section: Tt16 Mediates the Crosstalk Between Endothelium And Nucellusmentioning

confidence: 87%

Section: Tt16 Represses Hva22d a Negative Regulator Of Autophagy Andmentioning

confidence: 99%

Section: Plant and Genetic Materialsmentioning

confidence: 99%

Section: Tt16 Promotes Nucellus Degenerationmentioning

confidence: 99%

Section: Plant and Genetic Materialsmentioning

confidence: 99%

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Endosperm and Nucellus Develop Antagonistically in Arabidopsis Seeds

et al. 2016

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Regulatory Genes in Plant Development: MADS

Mā

2009

Encyclopedia of Life Sciences

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MADS‐box genes are found in all major groups of eukaryotes and encode known or putative transcription factors. MADS‐box genes have been phylogenetically defined as type I and type II genes. In plants, type II MADS‐domain proteins typically have the MADS, Intervening, K‐domain, C ‐terminal (MIKC) domain organization, with conserved MADS and K domains. Many of the plant type II MADS‐box genes have important regulatory functions in development, including the specification of flower organ identities, the control of flowering time, the regulation of meristem identity and the promotion of reproductive organ development. Recent studies have provided further insights into the regulation of MADS‐box genes and uncovered additional functions in controlling the development of various structures, such as embryo, endosperm, gametophyte and roots. In addition, protein physical interaction and genetic studies support the hypothesis that MADS‐box proteins form multimeric protein complexes that regulate specific target genes.

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Phenylpropanoid Metabolism in Plants: Biochemistry, Functional Biology, and Metabolic Engineering

Iandolino

Cook

2009

Plant Phenolics and Human Health

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The TRANSPARENT TESTA16 Locus Encodes the ARABIDOPSIS BSISTER MADS Domain Protein and Is Required for Proper Development and Pigmentation of the Seed Coat

Cited by 316 publications

References 69 publications

Endosperm and Nucellus Develop Antagonistically in Arabidopsis Seeds

Endosperm and Nucellus Develop Antagonistically in Arabidopsis Seeds

Regulatory Genes in Plant Development: MADS

Phenylpropanoid Metabolism in Plants: Biochemistry, Functional Biology, and Metabolic Engineering

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