The identification of a robust leucine dehydrogenase from a directed soil metagenome for efficient synthesis of L‐2‐aminobutyric acid

Liu, Yan; Zhong, X H; Luo, Zhidan; Meng, Xiangjing; Li, Rui; Wu, Zhong; Yang, Lin; Wang, Hualei; Wei, Dongzhi

doi:10.1002/biot.202200590

Cited by 3 publications

(2 citation statements)

References 36 publications

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“…An especially important discovery on the asteroid is also the presence of L-2-aminobutyric acid as a chiral precursor for the synthesis of non-natural L-amino acids in the later stage of origin [62,63,105]. The discovery of extra-terrestrial abiotic amino acids confirms our hypothesis that for the origin of life on Earth, all 20 natural amino acids were necessary.…”

Section: Discussionsupporting

confidence: 76%

The Evolution of Life Is a Road Paved with the DNA Quadruplet Symmetry and the Supersymmetry Genetic Code

Rosandić

Paar

2023

IJMS

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Symmetries have not been completely determined and explained from the discovery of the DNA structure in 1953 and the genetic code in 1961. We show, during 10 years of investigation and research, our discovery of the Supersymmetry Genetic Code table in the form of 2 × 8 codon boxes, quadruplet DNA symmetries, and the classification of trinucleotides/codons, all built with the same physiochemical double mirror symmetry and Watson–Crick pairing. We also show that single-stranded RNA had the complete code of life in the form of the Supersymmetry Genetic Code table simultaneously with instructions of codons’ relationship as to how to develop the DNA molecule on the principle of Watson–Crick pairing. We show that the same symmetries between the genetic code and DNA quadruplet are highly conserved during the whole evolution even between phylogenetically distant organisms. In this way, decreasing disorder and entropy enabled the evolution of living beings up to sophisticated species with cognitive features. Our hypothesis that all twenty amino acids are necessary for the origin of life on the Earth, which entirely changes our view on evolution, confirms the evidence of organic natural amino acids from the extra-terrestrial asteroid Ryugu, which is nearly as old as our solar system.

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Section: Discussionsupporting

confidence: 76%

The Evolution of Life Is a Road Paved with the DNA Quadruplet Symmetry and the Supersymmetry Genetic Code

Rosandić

Paar

2023

IJMS

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“…Gene mining is probabilistic, and it is difficult to find an enzyme with the same function. [22] Bioinformation analysis is necessary, which would contribute to reduced candidate homologues for functional verification.…”

Section: Screening Of Novel Ugt For Reb D Synthesis By Bioinformatics...mentioning

confidence: 99%

A sustainable strategy for biosynthesis of Rebaudioside D using a novel glycosyltransferase of Solanum tuberosum

Ma,

2024

Biotechnology Journal

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Bioconversion of Rebaudioside D faces high‐cost obstacles. Herein, a novel glycosyltransferase StUGT converting Rebaudioside A to Rebaudioside D was screened and characterized, which exhibits stronger affinity and substrate specificity for Rebaudioside A than previously reported enzymes. A whole‐cell catalytic system was thus developed using the StUGT strain. The production of Rebaudioside D was enhanced significantly by enhancing cell permeability, and the maximum production of 6.12 g/L and the highest yield of 98.08% by cell catalyst was obtained by statistical‐based optimization. A new cascade process utilizing this recombinant strain and E. coli expressing sucrose synthase was further established to reduce cost through replacing expensive UDPG with sucrose. A StUGT‐GsSUS1 system exhibited high catalytic capability, and 5.27 g L−1 Rebaudioside D was achieved finally without UDPG addition by systematic optimization. This is the best performance reported in cell‐cascaded biosynthesis, which paves a new cost‐effective strategy for sustainable synthesis of scarce premium sweeteners from biomass.

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Inside-Out Rational Design of Ornithine Cyclodeaminase RlOCD from Rhizobium leguminosarum by a Multiregion Synergy Strategy for Efficient Synthesis of l-Pipecolic Acid

Gao,

Jing,

Meng

et al. 2024

J. Agric. Food Chem.

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Lysine cyclodeaminase (LCD)-mediated synthesis of l-pipecolic acid (l-PA) from l-lysine (l-Lys) is a promising approach. However, only one LCD has been reported, and its inadequate activity limits industrial applications. To address this problem, a substrate analogue-guided enzyme mining strategy was employed. A novel ornithine cyclodeaminase (OCD) from Rhizobium leguminosarum (RlOCD) was identified in combination with directed macrogenomic approaches. RlOCD displayed a conversion rate of 28% at a substrate loading as high as 1000 mM. A multiregion synergy strategy consisting of pocket reshaping, dynamical cross-correlation matrix-guided coevolutionary design, and surface modification was used to design RlOCD from the inside-out. A quadruple mutant (V93C/L119C/I170T/R90L) designated Mu4 with significantly increased activity was obtained, which showed a 28.46-fold increase in the catalytic efficiency. The conversion of Mu4 was 91% within 10 h at 1000 mM (146.19 g L–1) loading. The space–time yield of 282.1 g L–1 d–1 is the highest level ever reported. Molecular dynamics simulations and interaction analyses revealed that efficient pocket expansion and unique conformational rearrangements increased the affinity for the substrate, resulting in a more catalytically active conformation. This study expands the toolbox for the production of l-PA and demonstrates the effectiveness and potential of Mu4 for its production.

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The identification of a robust leucine dehydrogenase from a directed soil metagenome for efficient synthesis of L‐2‐aminobutyric acid

Cited by 3 publications

References 36 publications

The Evolution of Life Is a Road Paved with the DNA Quadruplet Symmetry and the Supersymmetry Genetic Code

The Evolution of Life Is a Road Paved with the DNA Quadruplet Symmetry and the Supersymmetry Genetic Code

A sustainable strategy for biosynthesis of Rebaudioside D using a novel glycosyltransferase of Solanum tuberosum

Inside-Out Rational Design of Ornithine Cyclodeaminase RlOCD from Rhizobium leguminosarum by a Multiregion Synergy Strategy for Efficient Synthesis of l-Pipecolic Acid

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