The immune status, oxidative and epigenetic changes in tissues of turkeys fed diets with different ratios of arginine and lysine

Ognik, Katarzyna; Mikulski, D.; Konieczka, Paweł; Tykałowski, Bartłomiej; Krauze, M; Stępniowska, Anna; Nynca, Anna; Jankowski, Jan

doi:10.1038/s41598-021-95529-y

Cited by 6 publications

(4 citation statements)

References 44 publications

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“…Therefore, they do not contribute to chronic stimulation that reduces the efficiency of the immune system. Analysis of the immune and redox status of the small intestinal wall of birds do not corroborate the results of our previous study [ 40 ] where turkeys were fed diets with different Arg:Lys ratios relative to Met. In the cited study, no significant interactions were found between different dietary proportions of Arg and Lys vs. the immune response and the antioxidant status in the intestinal wall and blood of turkeys.…”

Section: Discussioncontrasting

confidence: 90%

Increased arginine, lysine, and methionine levels can improve the performance, gut integrity and immune status of turkeys but the effect is interactive and depends on challenge conditions

Konieczka

Tykałowski

Ognik

et al. 2022

Vet Res

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Arginine (Arg), lysine (Lys), and methionine (Met) can be used to support the health status of turkeys. The present study investigated selected performance, gut integrity, and immunological parameters in turkeys reared in optimal or challenge conditions. The experiment lasted for 28 days, and it had a completely randomized 2 × 3 factorial design with two levels of dietary Arg, Lys and Met (high or low) and challenge with Clostridium perfringens (C. perfringens), Escherichia coli lipopolysaccharide (LPS) or no challenge (placebo). Increased dietary levels of Arg, Lys and Met had a beneficial effect on turkey performance and immunological parameters, and it improved selected indicators responsible for maintaining gut integrity in different challenge conditions. Under optimal conditions (with no challenge), high ArgLysMet diets did not compromise bird performance and they improved selected performance parameters in challenged birds. The immune system of turkeys was not excessively stimulated by high ArgLysMet diets, which did not disrupt the redox balance and had no negative effect on gut integrity. High ArgLysMet diets increased the expression levels of selected genes encoding nutrient transporters and tight junction proteins. However, the influence exerted by different dietary inclusion levels of Arg, Lys and Met on gut integrity was largely determined by the stressor (C. perfringens vs. LPS). Further studies are required to investigate the role of Arg, Lys and Met levels in the diet on the immune response, gut function and performance of turkeys in different challenge conditions.

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Section: Discussioncontrasting

confidence: 90%

Increased arginine, lysine, and methionine levels can improve the performance, gut integrity and immune status of turkeys but the effect is interactive and depends on challenge conditions

Konieczka

Tykałowski

Ognik

et al. 2022

Vet Res

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“…Доказано, что введение аргинина способствует снижению уровней IL-6 и моноцитарного хемоаттрактанта. Установлено, что регуляция доступности аргинина является потенциальным механизмом, контролирующим продукцию NO, активацию iNOS и аргиназы, отражающим тип воспалительного ответа в условиях патологического состояния [18]. Наряду с этим, аргинин служит предшественником многочисленных биологически активных соединений таких как, оксида азота, орнитина, пролина и полиаминов, креатина и, следовательно, фосфокреатина и агматина, которые так или иначе участвуют в реализации иммунных механизмов.…”

Section: Victim Animalsunclassified

Evaluation of the immunotropic activity of neuropeptide compound Lys-Lys-Arg-Arg-Pro-Gly-Pro in conditions of "social" stress

Ясенявская¹,

Цибизова²,

Andreeva³

et al. 2023

Humans and their Health

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Objective: to investigate immunotropic activity of the argenine-containing neuropeptide compound Lys-Lys-Arg-Arg-Pro-Gly-Pro in conditions of "social" stress. Material and methods. The study was conducted on mongrel male rats of 6 months of age using a model of "social" stress based on the provision of permanent residence of animals in conditions of sensory contact, as a result of which submissive and aggressive types of behavior were formed. All animals were divided into groups: control I - rats who were in a cage one at a time and received water for injection in an equivalent volume; control II - groups of stressed animals with aggressive and submissive types of behavior; three groups of stressed rats were intraperitoneally injected with Lys-Lys-Arg-Arg-Pro-Gly-Pro at a dose of 100 mcg/kg/day and the comparison drug Imunofan (Arginyl-alpha-aspartyl-lysyl-valyl-thyrosyl-argininum) at an average therapeutic dose of 0.7 mcg/kg/day. The following indicators were evaluated: delayed hypersensitivity reaction index, antibody titer of direct hemagglutination reaction, phagocytic activity of peripheral blood neutrophils, leukocyte formula indicators. Results. The results obtained in the course of this study indicate that the arginine-containing neuropeptide compound Lys-Lys-Arg-Arg-Pro-Gly-Pro contributes to the correction of immune changes that occur against the background of exposure to chronic "social" stress, namely, an increase in the delayed hypersensitivity reaction index, the level of anti-erythrocyte antibodies, the total number of leukocytes and a decrease in phagocytic activity of peripheral blood neutrophils (phagocytic number and phagocytic index), as well as restoration of the percentage ratio of elements of the leukocyte formula. Conclusion. Thus, the study of the immunotropic activity of the argenine-containing neuropeptide compound Lys-Lys-Arg-Arg-Pro-Gly-Pro showed the presence of an immunocorrecting effect on the cellular and humoral links of immunity, which actualizes the need for a detailed study of the immune mechanisms of this compound.

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“…DL-methionine contained 990 g methionine/kg (MetAMINO ® ; Evonik Degussa GmbH, Essen, Germany). 1 Source: Ognik et al [ 17 ]. 2 L Low A Low , diet with low Lys and low Arg levels; L Low A High , diet with low Lys and high Arg levels; L High A Low , diet with high Lys and low Arg levels; L High A High , diet with high Lys and high Arg levels.…”

Section: Tablementioning

confidence: 99%

Increased Dietary Inclusion Levels of Lysine Are More Effective than Arginine in Supporting the Functional Status of the Gut in Growing Turkeys

Konieczka

Mikulski

Ognik

et al. 2021

Animals

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Arginine (Arg) and lysine (Lys) may be important for the overall health of turkeys. The aim of this study was to determine whether low (consistent with the guidelines) and high (10% higher than recommended) levels of dietary Arg and Lys can modulate performance and the functional status of the gut. Female turkeys were allocated to four dietary treatments (two levels of Lys (low or high) and two levels of Arg (low or high)) for a 16 wk feeding period. The treatments did not affect turkey performance determined separately for four feeding phases and for the entire 16 wk experiment (p > 0.05). They had no significant influence on carcass yield, meat characteristics or the associated traits either (p > 0.05). High-Lys diets contributed to a decrease in cecal pH, a significant increase in the concentrations of short-chain fatty acids (SCFA) and a decrease in the concentrations of putrefactive SCFA and ammonia in the cecum. High dietary levels of both amino acids significantly enhanced the activity of cecal microbiota evaluated based on extracellular enzyme activity. These findings indicate that the higher dietary level of Lys was more effective in modulating the physiological status of the gut in turkeys than Arg.

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The immune status, oxidative and epigenetic changes in tissues of turkeys fed diets with different ratios of arginine and lysine

Cited by 6 publications

References 44 publications

Increased arginine, lysine, and methionine levels can improve the performance, gut integrity and immune status of turkeys but the effect is interactive and depends on challenge conditions

Increased arginine, lysine, and methionine levels can improve the performance, gut integrity and immune status of turkeys but the effect is interactive and depends on challenge conditions

Evaluation of the immunotropic activity of neuropeptide compound Lys-Lys-Arg-Arg-Pro-Gly-Pro in conditions of "social" stress

Increased Dietary Inclusion Levels of Lysine Are More Effective than Arginine in Supporting the Functional Status of the Gut in Growing Turkeys

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