The in vitro and in vivo antitumor effects of Dracaena cinnabari resin extract on oral cancer

Al-Afifi, Nashwan Abdullah; Alabsi, Aied M.; Shaghayegh, Gohar; Ramanathan, Anand; Ali, Rola; Al-koshab, May; Bakri, Marina Mohd

doi:10.1016/j.archoralbio.2019.05.030

Cited by 10 publications

(5 citation statements)

References 57 publications

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“…Chondrocyte apoptosis was evaluated using the AO-PI double fluorescence staining assay [ 20 ]. Before the experiment, 1 μL each of AO dye and PI dye (both at 1 mg/mL) were mixed in PBS buffer.…”

Section: Methodsmentioning

confidence: 99%

Icariin alleviates osteoarthritis by regulating autophagy of chondrocytes by mediating PI3K/AKT/mTOR signaling

Tang

Li²,

Xin

et al. 2021

Bioengineered

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Osteoarthritis (OA) is a chronic degenerative disease that significantly impacts the quality of life of the elderly population. Recently, the pathogenesis of OA has been reported to involve autophagy in chondrocytes. Intriguingly, icariin, one of the main components of epimedium, exerts multiple pharmacological effects, including a protective effect against chondrocyte damage. Thus, we aimed to investigate the therapeutic effect of icariin on OA and its potential underlying mechanism by using a rat model of OA. After treatment with icariin or an autophagy activator (rapamycin) or inhibitor (3-methyladenine), OA chondrocyte viability was measured using the CCK-8 assay, apoptosis in the chondrocytes was evaluated using the acridine orange-propidium iodide assay and flow cytometry, and OA tissue pathological state was assessed using micro-CT scanning and safranin O staining. Furthermore, immunohistochemical staining was used to measure the expression level of Beclin-1 and immunofluorescence labeling was used to visualize LC3 expression, and western blotting was used to determine the expression levels of autophagy proteins and key proteins in the PI3K signaling pathway. The apoptotic rate of OA chondrocytes was markedly elevated by 3-methyladenine and suppressed by rapamycin and icariin; autophagy genes were drastically downregulated in the 3-methyladenine group and upregulated in the rapamycin and icariin groups; and the PI3K/AKT/mTOR signaling pathway was activated by 3-methyladenine and inhibited by rapamycin and icariin. Notably, following treatment with rapamycin and icariin, the severe pathological state in OA cartilage tissues was substantially alleviated, and this was accompanied by activated autophagy and inhibited PI3K signaling in the cartilage tissues. Taken together, these findings indicate that icariin alleviates OA by regulating the autophagy of chondrocytes by mediating PI3K/AKT/mTOR signaling.

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Section: Methodsmentioning

confidence: 99%

Icariin alleviates osteoarthritis by regulating autophagy of chondrocytes by mediating PI3K/AKT/mTOR signaling

Tang

Li²,

Xin

et al. 2021

Bioengineered

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“…In experimented tongue squamous cell carcinoma cells (H103), methanolic extract of D. cinnabari resulted in morphological changes, induction of apoptotic conditions, and cell cycle arrest (S and G2/M phase). It was noticed that the incidence of squamous cell carcinoma in induced oral cancer was 85.7%; however, groups that were administrated with methanolic extract (100, 500, and 100 mg kg -1 ) of dragon's blood resin were 57.1%, 28.6%, and 14.3%, respectively [60]. They further stated that this anticancer effect of D. cinnabari may be due to inhibition of p-53, Cox-2 Bcl-2, and cyclin D1 expression and upregulation of Casp-3 and Bax genes [61].…”

Section: Anti-inflammatory and Analgesic Effects Alwashli Et Almentioning

confidence: 97%

Phytochemistry and Pharmacological Activities of Dracaena cinnabari Resin

Al-Awthan

Bahattab

2021

BioMed Research International

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Dracaena cinnabari (D. cinnabari) is an endemic plant located in Socotra Island, Yemen. Deep red resin attained from different plant species including D. cinnabari is commonly known as dragon’s blood. In folk medicine, it is prescribed for the treatment of traumatic dermal, dental, and eye injuries as well as blood stasis, pain, and gastrointestinal diseases in humans. Numerous studies have investigated that this resinous medicine has antidiarrheal, antiulcer, antimicrobial, antiviral, antitumor, anti-inflammatory, analgesic, wound healing, and antioxidant activity. Several phytochemicals have been isolated from D. cinnabari, including the biflavonoid cinnabarone, triflavonoids, metacyclophanes, chalcones, chalcanes, dihydrochalcones, sterols, and terpenoids. The present review highlights the structures and bioactivities of main phytochemicals isolated from D. cinnabari regarding the botany and pharmacological effects of the resin derived from this plant.

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“…After treatment of H103 cells, morphological changes and cell migration reduction were observed, apoptosis was induced through the intrinsic (mitochondrial) pathway, and G2/M and S phase cell cycle arrest was observed. In an in vivo study, D. cinnabari methanolic extract-treated rats showed incidences of SCC of 500 mg/kg (28.6%), 100 mg/kg (57.1%) and 1000 mg/kg (14.3%) compared to 4NQO-induced cancer rats (85.7%) [ 61 ].…”

Section: Plants With Beneficial Effects Against Oral Cancermentioning

confidence: 99%

Plant-Based Antioxidant Extracts and Compounds in the Management of Oral Cancer

et al. 2021

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Oral cancer continues to be a leading cause of death worldwide, and its prevalence is particularly high in developing countries, where people chew tobacco and betel nut on a regular basis. Radiation-, chemo-, targeted-, immuno-, and hormone-based therapies along with surgery are commonly used as part of a treatment plan. However, these treatments frequently result in various unwanted short- to long-term side effects. As a result, there is an urgent need to develop treatment options for oral cancer that have little or no adverse effects. Numerous bioactive compounds derived from various plants have recently attracted attention as therapeutic options for cancer treatment. Antioxidants found in medicinal plants, such as vitamins E, C, and A, reduce damage to the mucosa by neutralizing free radicals found in various oral mucosal lesions. Phytochemicals found in medicinal plants have the potential to modulate cellular signalling pathways that alter the cellular defence mechanisms to protect normal cells from reactive oxygen species (ROS) and induce apoptosis in cancer cells. This review aims to provide a comprehensive overview of various medicinal plants and phytoconstituents that have shown the potential to be used as oral cancer therapeutics.

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The in vitro and in vivo antitumor effects of Dracaena cinnabari resin extract on oral cancer

Cited by 10 publications

References 57 publications

Icariin alleviates osteoarthritis by regulating autophagy of chondrocytes by mediating PI3K/AKT/mTOR signaling

Icariin alleviates osteoarthritis by regulating autophagy of chondrocytes by mediating PI3K/AKT/mTOR signaling

Phytochemistry and Pharmacological Activities of Dracaena cinnabari Resin

Plant-Based Antioxidant Extracts and Compounds in the Management of Oral Cancer

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