The in vitro isolated whole guinea pig brain as a model to study epileptiform activity patterns

Curtis, Marco de; Librizzi, Laura; Uva, Laura

doi:10.1016/j.jneumeth.2015.03.026

Cited by 19 publications

(5 citation statements)

References 47 publications

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“…US-induced BBB opening was performed on an isolated guinea pig brain maintained in vitro by arterial perfusion, a unique experimental preparation in which the BBB is morphologically and functionally preserved for several hours. [5] The US system mounted a planar transducer with nominal frequency of 0.989 MHz. Sonications were carried out for 2 consecutive minutes with intensity from 20 to 100 mW/cm2, pulsed for 100 ms ON, 900 ms OFF duty cycle (1 kHz pulse repetition rate).…”

Section: Methodsmentioning

confidence: 99%

Quantitative analysis of CEUS guided blood-brain barrier opening in an in vitro isolated brain preparation

Gionso,

Librizzi,

Uva

et al. 2022

Proceedings of the 10th Convention of the European Acoustics Association Forum Acusticum 2023

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Section: Methodsmentioning

confidence: 99%

Quantitative analysis of CEUS guided blood-brain barrier opening in an in vitro isolated brain preparation

Gionso,

Librizzi,

Uva

et al. 2022

Proceedings of the 10th Convention of the European Acoustics Association Forum Acusticum 2023

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“…Brains from female Hartley guinea pigs (150‐200 g, Charles River, Calco, Italy) were isolated and maintained in vitro according to the standard procedure described elsewhere . Briefly, animals anesthetized with sodium thiopental (125 mg/kg i.p., Farmotal, Pharmacia, Milan, Italy) were trans‐cardially perfused with a cold (10°C), carboxygenated (95% O 2 , 5% CO 2 ) solution containing:126 mmol/L NaCl, 3 mmol/L KCl, 1.2 mmol/L KH 2 PO 4 , 1.3 mmol/L MgSO 4 , 2.4 mmol/L CaCl 2 , 26 mmol/L NaHCO 3 , 15 mmol/L glucose, 2.1 mmol/L HEPES, and 3% dextran M.W.…”

Section: Methodsmentioning

confidence: 99%

Enhanced thalamo‐hippocampal synchronization during focal limbic seizures

et al. 2018

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“…Forty adult male guinea pigs, weighting 400-500g were employed in the present work. Researchers found that guinea pigs are the best experimental model to study epileptic activity in the brain and they offer experimental advantages not achieved with use of other experimental models [13] . They were housed at room temperature in stainless steel cages.…”

Section: Animalsmentioning

confidence: 99%

Histological and immunohistochemical study on the effect of valporic acid on the bone of adult male guinea pigs and the possible protective role of L-carnitine

Ali

2019

Egyptian Journal of Histology

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Background: Valporic acid (VPA) is a broad-spectrum antiepileptic drug and is effective in the treatment of different types of epileptic seizures. It usually affects bone and mineral metabolism and increase the risk of fractures due to decreased bone mineral density (BMD). Among dietary supplements, L-carnitine is an emergic candidate with potential bone protective effects. It promotes energy utilization and is important for tissues with high energy requirements. Objective: The target of this work is to estimate the effect of valproic acid on the femur and the possible protective effect of L-carnitine administration in adult male guinea pigs. Materials & Methods: Forty adult male guinea pigs were utilized in the current study. The guinea pigs were divided into four groups, Group I (control group), Group II(L-carnitine treated group), Group III (Valporic acid treated group) and Group IV (L-carnitine and valporic acid treated group). After 10 weeks, the upper parts of femur were processed for histological, morphological and immunohistochemical studies. Results: Valporic acid treated group was associated with enhanced bone turnover as evident by a significant change in serum levels of calcium, osteocalcin and TRAP. Moreover, there was a significant decrease in trabecular and cortical bone thickness and a significant change in the mean number of osteoclasts and osteoblasts. Histologically, evidence of bone resorption was manifested in the femoral bone with resorption cavities, irregular endosteal surface. Decrease of collagen in the cortical bone was evident in trichrome-stained sections. Immunohistochemically, this group showed positive immunoreactivity for caspase-3 in osteocytes and decrease in osteopontin expression in bone matrix. L-carnitine supplementation with valporic acid in group IV has ameliorating effect on the histological abnormalities of bone. Conclusion: L-carnitine improved the biochemical, histological and morphometric changes induced by VPA, and therefore, there is a potential benefit in using L-carnitine with long term administration of valporic acid.

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The in vitro isolated whole guinea pig brain as a model to study epileptiform activity patterns

Cited by 19 publications

References 47 publications

Quantitative analysis of CEUS guided blood-brain barrier opening in an in vitro isolated brain preparation

Quantitative analysis of CEUS guided blood-brain barrier opening in an in vitro isolated brain preparation

Enhanced thalamo‐hippocampal synchronization during focal limbic seizures

Histological and immunohistochemical study on the effect of valporic acid on the bone of adult male guinea pigs and the possible protective role of L-carnitine

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