The in vitro propagation system of Citrus × latifolia (Yu. Tanaka) Yu. Tanaka (Rutaceae) affects the growth and depletion of nutriments

Bulbarela-Marini, Javier Emanuel; Gómez-Meriño, Fernando Carlos; Galindo-Tovar, María Elena; Solano-Rodríguez, Luis Alberto; Murguía-González, Joaquín; Pastelín-Solano, Miriam Cristina; Núñez-Pastrana, Rosalía; Castañeda-Castro, Odón

doi:10.1007/s11627-019-09976-4

Cited by 7 publications

(1 citation statement)

References 27 publications

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“…These authors suggested that the extracellular chemical compounds produced by the cultivations stimulate the formation of shoots, which would be more diluted if larger volumes of medium are used. In Citrus x latifolia, Bulbarela-Marini et al (2019) obtained the best results as regards the number of developed shoots and their size when using higher volumes per explant (40 ml). In Stevia rebaudiana, (Bayraktar 2019), achieved 4 times more shoots than in semi-solid medium on using a higher volume of medium per container (300 ml).…”

Section: Shoot Numbermentioning

confidence: 98%

Temporary immersion systems to improve alder micropropagation

José

Blázquez

Cernadas

et al. 2020

Plant Cell Tiss Organ Cult

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A protocol has been developed for the propagation of Alnus glutinosa axillary shoots in liquid medium. The explants were cultured in Woody Plant Medium supplemented with 0.1 mg l − 1 benzyladenine and 0.5 mg l − 1 indole acetic acid. The effect of the bioreactor type (RITA® and Plantform™), frequency of the immersions, regulator concentrations, and volume of medium per explant was investigated. All the treatments with the temporal immersion systems (TIS) increased the proliferation rates. The best results were obtained on using the Plantform™ vessels and, unlike the shoots cultured in RITA® vessels, the culture in Plantform™ had very low hyperhydricity percentages. Shoots originating from the culture in semisolid medium and in Plantform™ rooted in semi-solid medium with 0.1 mg/l indole butyric acid for 7 days. No significant differences were observed in the rooting or acclimatization percentages, with survival percentages greater than 85% being achieved. This is the first work on the use of TIS systems in alder propagation, with the results of this study providing new perspectives for its mass propagation. Key messageA new protocol is described in order to reduce the propagation costs of alder.

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Section: Shoot Numbermentioning

confidence: 98%