The in vitro stimulation of lymphocytes from peripheral blood and lymph nodes of the laboratory mouse

Abstract

The results of comparative studies of the efficacy of phytohemagglutinin (PHA-P) and pokeweed mitogen (PWM) as mitotic stimulators for in vitro cultures of mouse lymphocytes are reported. These studies have established that clearly defined optimal concentration ranges exist for each mitogen and that higher than optimal levels of either mitogen exert an inhibitory effect on cell proliferative activities. The levels of mitotic stimulation achieved with peripheral-blood cultures and with cultures of lymph-node lymphocytes should facilitate a variety of cytogenetic studies not heretofore practicable with the mouse.

Stimulation of Peripheral Blood Lymphocytes by Herpes Simplex Virus in Vitro

“…Whole-blood cultures have been applied previously for lymphocyte stimulation by mitogens (4,10,12,17,(22)(23)(24) or various antigens (5,17,23,24). Most of the authors recommend methods which consist of simply diluting the anti-coagulated blood in serum-free medium.…”