The Increases in mRNA Expressions of Inflammatory Cytokines by Adding Cleaning Solvent or Tetrachloroethylene in the Murine Macrophage Cell Line J774.1 Evaluated by Real-time PCR

Kido, Takamasa; Sugaya, Chiemi; Ikeuchi, Ryutaro; Kudo, Yuichiro; Tsunoda, Masashi; Aizawa, Yoshifusa

doi:10.2486/indhealth.ms1364

Cited by 4 publications

(2 citation statements)

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“…Targets were amplified on a LightCycler Nano (Roche Diagnostics) using LightCycler FastStart DNA Master PLUS SYBR Green I (Roche Diagnostics) over 40 cycles of denaturation at 94°, annealing at temperatures indicated in Table , and extension at 72°. Calibrator‐normalized cytokine to 18S rRNA relative ratio was calculated as the index of mRNA expression of each cytokine, following the manufacturer's protocols (Roche) …”

Section: Methodsmentioning

confidence: 99%

Inflammatory response under zinc deficiency is exacerbated by dysfunction of the T helper type 2 lymphocyte–M2 macrophage pathway

et al. 2019

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Nutritional zinc deficiency leads to immune dysfunction and aggravates inflammation. However, the underlying mechanism remains unknown. In this study, the relationship between macrophage subtypes (M1 and M2) and helper T lymphocytes (Th1 and Th2) was investigated using the spleen from rats fed zinc-deficient or standard diet. In experiment I, 5week-old male Sprague-Dawley rats were fed a zinc-deficient diet (without zinc additives) or a standard diet (containing 0Á01% zinc) for 6 weeks. In experiment II, the rats were divided into four groups: one group was fed a standard diet for 6 weeks; two groups were fed zinc-deficient diets and were injected three times a week with either saline or interleukin-4 (IL-4) (zinc-deficient/IL-4 i.p.); a fourth group (zinc-deficient/standard) was fed a zinc-deficient diet for 6 weeks followed by a standard diet for 4 weeks. In experiment I; GATA-binding protein 3 (GATA-3) protein level, M2 macrophage, CD3 + CD8 + cells, and IL-4/IL-13-positive cells significantly decreased in the spleens of the zinc-deficient group. Additionally, IL-1b and macrophage inflammatory protein-1a (MIP-1a) mRNA levels significantly increased in the splenic macrophages of the zinc-deficient group. In experiment II; M2 macrophages, CD3 + CD8 + cells, IL-4/IL-13-positive cells, and GATA-3 protein levels significantly increased in the spleens of the zinc-deficient/IL-4 i.p. and zinc-deficient/standard groups. Furthermore, IL-1b and MIP-1a mRNA levels decreased in the splenic macrophages of the zinc-deficient/IL-4 i.p. and zinc-deficient/standard groups. Zinc deficiency-induced aggravated inflammation is related to Th2 lymphocytes and followed by the association with loss of GATA-3, IL-4 and anti-inflammatory M2 macrophages. Importantly, IL-4 injection or zinc supplementation can reverse the effects of zinc deficiency on immune function.

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Section: Methodsmentioning

confidence: 99%

Inflammatory response under zinc deficiency is exacerbated by dysfunction of the T helper type 2 lymphocyte–M2 macrophage pathway

et al. 2019

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“…These data suggest possible mechanisms for tetrachloroethylene-induced perturbation of the immune response to allergens, and exacerbation of inflammation. An in vitro study by Kido et al (2013) also showed effects on pro-inflammatory cytokine gene expression. Significant (p<0.05) increases in the expression of IL-6…”

Section: Renal Effectsmentioning

confidence: 92%

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Brooke¹

2014

Essentials of Environmental Public Health Science

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Managing Hazardous Materials Incidents is a three-volume set of recommendations for on-scene (prehospital) and hospital medical management of patients exposed during a hazardous materials incident (see https://www.atsdr.cdc.gov/MHMI/index.asp). Volumes I and II are planning guides to assist first responders and hospital emergency department personnel in planning for incidents that involve hazardous materials. Volume III-Medical Management Guidelines for Acute Chemical Exposures-is a guide for health care professionals treating patients exposed to hazardous materials.Fact Sheets (ToxFAQs™) provide answers to frequently asked questions about toxic substances (see https://www.atsdr.cdc.gov/toxfaqs/Index.asp). Other Agencies and OrganizationsThe National Center for Environmental Health (NCEH) focuses on preventing or controlling disease, injury, and disability related to the interactions between people and their environment outside the workplace. Contact: NCEH,

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Fluoride potentiates tubulointerstitial nephropathy caused by unilateral ureteral obstruction

et al. 2017

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The Increases in mRNA Expressions of Inflammatory Cytokines by Adding Cleaning Solvent or Tetrachloroethylene in the Murine Macrophage Cell Line J774.1 Evaluated by Real-time PCR

Cited by 4 publications

References 11 publications

Inflammatory response under zinc deficiency is exacerbated by dysfunction of the T helper type 2 lymphocyte–M2 macrophage pathway

Inflammatory response under zinc deficiency is exacerbated by dysfunction of the T helper type 2 lymphocyte–M2 macrophage pathway

Physicochemical properties

Fluoride potentiates tubulointerstitial nephropathy caused by unilateral ureteral obstruction

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