The infiltration and functional regulation of eosinophils induced by TSLP promote the proliferation of cervical cancer cell

Xie, Feng; Liu, Libing; Shang, Wen-Qing; Chang, Kai‐Kai; Meng, Yuguang; Mei, Jie; Yu, Jiajun; Li, Da‐Jin; Li, Ming‐Qing

doi:10.1016/j.canlet.2015.04.029

Cited by 82 publications

(76 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…HL-60 cells, a promyelocytic leukemia cell line, were purchased from the Type Culture Collection of the Chinese Academy of Sciences. According to a previous study (19), the HL-60-eosinophils (HL-60E) were generated by incubating HL-60 cells for 2 months at pH 7.7 in RPMI-1640 medium containing 25 mM N-(2-hydroxyethyl) piperazine-N'-3-propane-sulfonic acid (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) and subsequently culturing for 7 days in RPMI-1640 medium with 0.5 mM butyric acid (Sigma-Aldrich; Merck KGaA). HUVECs (Type Culture Collection of the Chinese Academy of Sciences) were maintained as monolayers in Dulbecco's modified Eagle medium/F-12 medium (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS.…”

Section: Methodsmentioning

confidence: 99%

“…Our previous study demonstrated that TSLP secreted by cervical cancer cells promotes angiogenesis (18). In addition, an increased level of TSLP in cancer lesions, mediated by hypoxia, was a regulator of the progression of cervical cancer, and functioned by recruiting and enabling tumor-associated eosinophils (EOS) to promote the growth of the cervical cancer cells (19). However, it remains unknown whether and how TSLP, derived from cervical cells, regulates angiogenesis by modulating the crosstalk between cervical cancer cells and EOS.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

TSLP promotes angiogenesis of human umbilical vein endothelial cells by strengthening the crosstalk between cervical cancer cells and eosinophils

et al. 2017

Self Cite

View full text Add to dashboard Cite

Abstract. Our previous study demonstrated that thymic stromal lymphopoietin (TSLP) secreted by cervical cancer cells promotes angiogenesis and recruitment, and regulates the function of eosinophils (EOS). However, the function of TSLP in the crosstalk between EOS and vascular endothelial cells in cancer lesions remains unknown. The aim of the present study was to investigate the effect of EOS caused by TSLP in in vitro angiogenesis of human umbilical vein endothelial cells (HUVECs). The results of the present study revealed that recombinant human TSLP protein (rhTSLP) increased the secretion of vascular endothelial growth factor (VEGF), but not fibroblast growth factors, in HL-60-eosinophils (HL-60E). Compared with cervical cancer cells (HeLa or CasKi cells) or HL-60E alone, there were increased levels of interleukin (IL)-8 and VEGF in the co-culture system between cervical cancer cells, and HL-60E cells. This effect was strengthened by rhTSLP, but inhibited by inhibiting the TSLP signal with anti-human TSLP or TSLP receptor neutralizing antibodies. The results of the tube formation assays revealed that treatment with the supernatant from cervical cancer cells and/or HL-60E resulted in an increase in angiogenesis in HUVECs, which could be decreased by TSLP or TSLPR inhibitors. The results of the present study suggested that TSLP derived of cervical cancer cells may indirectly stimulate angiogenesis of HUVECs, by upregulating IL-8 and VEGF production, in a co-culture model between cervical cancer cells and EOS, therefore promoting the development of cervical cancer.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

TSLP promotes angiogenesis of human umbilical vein endothelial cells by strengthening the crosstalk between cervical cancer cells and eosinophils

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…Previous research has established that TSLP is aberrantly highly expressed in CC cells, indirectly promoting their growth by recruiting and regulating tumor-associated EOS, and stimulating angiogenesis in CC lesions (9)(10)(11). Additionally, hypoxia may contribute to the increase in the TSLP expression level in CC cells.…”

Section: Discussionmentioning

confidence: 99%

“…The high level of TSLP production is associated with local hypoxia (9). Notably, hypoxia also results in the upregulation of miR-132 (34,35).…”

Section: Discussionmentioning

confidence: 99%

“…The TSLP receptor (TSLPR) is a typical heterodimeric cytokine receptor, including a TSLP binding subunit (TSLPRα) and α-subunit of the interleukin-7 receptor (7,8). A previous study reported that an aberrantly high level of TSLP present in cancer lesions, potentially mediated by hypoxia, was a notable regulator of the progression of CC by recruiting and licensing tumor-associated eosinophils (EOS) to CC lesions (9). In addition, TSLP produced by CC cells promotes angiogenesis in an EOS-dependent and -independent manner (10,11).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Human thymic stromal lymphopoietin promotes the proliferation and invasion of cervical cancer cells by downregulating microRNA‑132 expression

et al. 2017

Self Cite

View full text Add to dashboard Cite

Abstract. Thymic stromal lymphopoietin (TSLP), produced by cervical cancer (CC) cells, promotes angiogenesis, and the recruitment and functional regulation of eosinophils. It has been reported that microRNA (miR)-132 is aberrantly decreased in CC tissues. However, the function and mechanism of TSLP on the biological behaviors of CC cells is largely unknown. The aim of the present study was to investigate the effect of TSLP on the expression of miR-132 and the proliferation and invasion in vitro of CC cell lines, namely, HeLa and SiHa cells. The transcrpitional level of miR-132 was analyzed using reverse transcription-quantitative polymerase chaon reaction. The proliferation, invasion, and the expression of proliferation and invasion-related molecules in HeLa and SiHa cells in vitro were evaluated using bromodeoxyuridine cell proliferation, Matrigel invasion assays, flow cytometry and ELISA, respectively. Here, it was revealed that recombinant human TSLP (rhTSLP) downregulated the expression levels of miR-132 in HeLa and SiHa cells, and by contrast, the neutralizing antibodies for TSLP or TSLP receptor (TSLPR) upregulated miR-132 expression levels in HeLa and SiHa cells. The overexpression of miR-132 resulted in a lowered proliferation and invasiveness, decreased levels of proliferation-associated molecules marker of proliferation Ki-67 and proliferating cell nuclear antigen, and the decreased production of matrix metalloproteinase (MMP)2 and MMP9 in HeLa and SiHa cells. Compared with the control group, there was a higher level of proliferation and invasion in HeLa and SiHa cells following stimulation with rhTSLP. However, these effects induced by rhTSLP were significantly impaired in HeLa and SiHa cells with miR-132 overexpression. The results of the present study indicated that TSLP produced by CC cells downregulated miR-132 expression, and stimulated the proliferation and invasion of CC cells, thereby further promoting the development of CC. IntroductionCervical cancer (CC) is one of the most common gynecological malignancies with high rates of morbidity (0.001-0.05%) and mortality (0.0024-0.0175%), endangering the health and quality of life of women globally (1). Although the decreased incidence of CC has primarily been attributed to the widespread use of cytological screening, invasive CC remains an issue regarding the mortality rates of patients with CC. Thus, the elucidation of the molecular pathogenesis of CC is urgently required. The pathogenesis of CC remains enigmatic and may be connected with numerous factors, including infection with high-risk human papillomaviruses (HPVs) (2).Thymic stromal lymphopoietin (TSLP) is primarily produced by stromal cells, epithelial cells, fibroblasts, keratinocytes, basophils and other types of cells, including mast cells, smooth muscle cells, fibroblasts, dendritic cells, trophoblasts, and cancer or cancer-associated cells (3-5). TSLP may trigger helper T-cell 2 cytokines, and is associated with airway inflammatory disease, immunoglobulin E production, allergic respons...

show abstract

From the bench to the clinic: basophils and type 2 epithelial cytokines of thymic stromal lymphopoietin and IL‐33

Obata‐Ninomiya,

Jayaraman,

Ziegler

2024

Clin & Trans Imm

View full text Add to dashboard Cite

Type 2 epithelial cytokines, including thymic stromal lymphopoietin and IL‐33, play central roles in modulation of type 2 immune cells, such as basophils. Basophils are a small subset of granulocytes within the leukocyte population that predominantly exist in the blood. They have non‐redundant roles in allergic inflammation in peripheral tissues such as the lung, skin and gut, where they increase and accumulate at inflammatory lesions and exclusively produce large amounts of IL‐4, a type 2 cytokine. These inflammatory reactions are known to be, to some extent, phenocopies of infectious diseases of ticks and helminths. Recently, biologics related to both type 2 epithelial cytokines and basophils have been approved by the US Food and Drug Administration for treatment of allergic diseases. We summarised the roles of Type 2 epithelial cytokines and basophils in basic science to translational medicine, including recent findings.

show abstract

The infiltration and functional regulation of eosinophils induced by TSLP promote the proliferation of cervical cancer cell

Cited by 82 publications

References 38 publications

TSLP promotes angiogenesis of human umbilical vein endothelial cells by strengthening the crosstalk between cervical cancer cells and eosinophils

TSLP promotes angiogenesis of human umbilical vein endothelial cells by strengthening the crosstalk between cervical cancer cells and eosinophils

Human thymic stromal lymphopoietin promotes the proliferation and invasion of cervical cancer cells by downregulating microRNA‑132 expression

From the bench to the clinic: basophils and type 2 epithelial cytokines of thymic stromal lymphopoietin and IL‐33

Contact Info

Product

Resources

About