The Influence of Application Protocol of Simplified and Universal Adhesives on the Dentin Bonding Performance

Zecin-Deren, Anna; Łukomska−Szymańska, Monika; Szczesio-Włodarczyk, Agata; Piwoński, Ireneusz; Sokołowski, Jerzy; Łapińska, Barbara

doi:10.3390/app10010124

Cited by 12 publications

(13 citation statements)

References 40 publications

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“…The included studies mostly used universal adhesives in self-etching mode when placing bulk-fill resins. These adhesives are gaining popularity among clinicians, allowing for simplified procedures, however their dentin bonding potential can be enhanced by modifying the application method [ 49 , 50 ]. One study [ 21 ] using the self-etch adhesive Xeno III attributed the post-operative sensitivity to the adhesive used rather than to the bulk-fill resin material.…”

Section: Discussionmentioning

confidence: 99%

Clinical Effectiveness of Bulk-Fill and Conventional Resin Composite Restorations: Systematic Review and Meta-Analysis

et al. 2020

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The objective of this systematic review and meta-analysis was to determine the clinical effectiveness of bulk-fill and conventional resin in composite restorations. A bibliographic search was carried out until May 2020, in the biomedical databases Pubmed/MEDLINE, EMBASE, Scopus, CENTRAL and Web of Science. The study selection criteria were: randomized clinical trials, in English, with no time limit, with a follow-up greater than or equal to 6 months and that reported the clinical effects (absence of fractures, absence of discoloration or marginal staining, adequate adaptation marginal, absence of post-operative sensitivity, absence of secondary caries, adequate color stability and translucency, proper surface texture, proper anatomical form, adequate tooth integrity without wear, adequate restoration integrity, proper occlusion, absence of inflammation and adequate point of contact) of restorations made with conventional and bulk resins. The risk of bias of the study was analyzed using the Cochrane Manual of Systematic Reviews of Interventions. Sixteen articles were eligible and included in the study. The results indicated that there is no difference between restorations with conventional and bulk resins for the type of restoration, type of tooth restored and restoration technique used. However, further properly designed clinical studies are required in order to reach a better conclusion.

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Section: Discussionmentioning

confidence: 99%

Clinical Effectiveness of Bulk-Fill and Conventional Resin Composite Restorations: Systematic Review and Meta-Analysis

et al. 2020

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“…Interestingly, based on both proportional composition as well as the chemistry of these components dental adhesives may be divided into different generations [1,4]. Dental adhesives, that constitute a multifunctional systems, may be used as self-etch adhesives, etch-and-rinse adhesives or in a selective enamel etching technique [5][6][7][8][9]. They are commonly used for a wide range of clinical application, whereas a several important factors should be considered before selection of the bonding procedure and adhesive system for the vital dentine.…”

Section: Introductionmentioning

confidence: 99%

The Cytotoxicity and Genotoxicity of Three Dental Universal Adhesives—An In Vitro Study

Wawrzynkiewicz

Rozpędek‐Kamińska

Galita

et al. 2020

IJMS

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Dental universal adhesives are considered an useful tool in modern dentistry as they can be used in different etching techniques, allow for simplified protocol and provide sufficient bond strength. However, there is still no consensus as to their toxicity towards pulp. Thus, the present study aimed to evaluate the cytotoxicity and genotoxicity of three universal adhesives: OptiBond Universal, Prime&Bond Universal and Adhese in an in vitro experimental model, monocyte/macrophage cell line SC (ATCC CRL-9855). The cytotoxicity was measured by means of XTT assay, whereas the genotoxicity (comet assay) was evaluated based on the percentage of DNA present in the comet tail. Furthermore, the ability of the adhesives to induce apoptosis was analyzed using flow cytometry (FC) with the FITC annexin V/propidium iodide (PI) double staining. The analysis of the cell cycle progression was performed with FC using PI staining. OptiBond Universal presented significant, while Prime&Bond Universal and Adhese Universal had minimal cytotoxicity and genotoxicity towards human SC cells. Moreover, only OptiBond Universal increased the level of apoptosis in SC cell line. None of the adhesives showed significant cell cycle arrest, as revealed by FC analysis. Due to substantial differences in toxicity in in vitro studies of dental adhesives, there is a great need for further research in order to establish more reliable test protocols allowing for standardized methodology.

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“…Moreover, by applying the adhesive with active rubbing motions, fresh monomers are constantly provided to enhance adhesive infiltration. Positive effects of altered application modes (active, prolonged or multiplied application) on the polymer-dentin bond strengths have been reported for universal adhesives in recent studies [8,24,25,30,39] and can be attributed to the better infiltration of monomers into decalcified dentin [8,38]. The fact that the low bond strengths of the group with halved acid etching time and halved adhesive application time (G2) could almost be raised to the level of the control group (G1) by 20 s of adhesive application time (G6) emphasizes the importance of strict adherence to the minimum recommended application time.…”

Section: Discussionmentioning

confidence: 93%

“…The extracted teeth were then cleaned [38] and stored in 0.5% chloramine-T solution [8,27,39]. In order to facilitate the subsequent use, the root tips were glued perpendicular to the center of a holder for scanning electron microscopes (Wenka, Karl Wenger SA, Courgenay, Switzerland) with superglue (Superglue No.…”

Section: Specimen Preparationmentioning

confidence: 99%

“…The achievement of a strong and solid hybrid layer plays an important role in bonding composite to dentin and is still a challenge during adhesive restorative procedures [1][2][3][4][5][6][7][8]. Establishing reliable adhesion to dentin is elaborate due to its high organic and water content, as well as low surface energy and the presence of a smear layer [7].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Over-Etching and Prolonged Application Time of a Universal Adhesive on Dentin Bond Strength

et al. 2020

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This study investigated the effect of over-etching and prolonged application time of a universal adhesive on dentin bond strength. Ninety extracted human molars were ground to dentin and randomly allocated into nine groups (G1–9; n = 10 per group), according to the following acid etching and adhesive application times. In the control group (G1), phosphoric acid etching was performed for 15 s followed by application of the universal adhesive Scotchbond Universal (3M) for 20 s, as per manufacturer’s instructions. In groups G2–5, both the etching and adhesive application times were either halved, doubled, quadrupled, or increased eightfold. In groups G6–9, etching times remained the same as in G2–5 (7.5 s, 30 s, 60 s, and 120 s, respectively), but the adhesive application time was set at 20 s as in the control group (G1). Specimens were then restored with a nanofilled composite material and subjected to microtensile bond strength testing. Bond strength data were statistically analyzed by ANOVA and Tukey’s post-hoc tests (α = 0.05). The relationship of bond strength with etching and adhesive application time was examined using linear regression analysis. Treatment of dentin with halved phosphoric acid etching and adhesive application times (G2) resulted in a significant bond strength decrease compared to the control group (G1) and all other test groups, including the group with halved acid etching, but 20 s of adhesive application time (G6). No significant differences in bond strength were found for groups with multiplied etching times and an adhesive application time of 20 s or more, when compared to the control group (G1). In conclusion, a universal adhesive application time of at least 20 s is recommended when bonding to over-etched dentin.

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The Influence of Application Protocol of Simplified and Universal Adhesives on the Dentin Bonding Performance

Cited by 12 publications

References 40 publications

Clinical Effectiveness of Bulk-Fill and Conventional Resin Composite Restorations: Systematic Review and Meta-Analysis

Clinical Effectiveness of Bulk-Fill and Conventional Resin Composite Restorations: Systematic Review and Meta-Analysis

The Cytotoxicity and Genotoxicity of Three Dental Universal Adhesives—An In Vitro Study

Effect of Over-Etching and Prolonged Application Time of a Universal Adhesive on Dentin Bond Strength

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