The influence of nano-scale surface roughness on bacterial adhesion to ultrafine-grained titanium

Truong, Vi Khanh; Lapovok, Rimma; Estrin, Yuri; Rundell, Stuart; Wang, James; Fluke, C. J.; Crawford, Russell J.; Ivanova, Elena P.

doi:10.1016/j.biomaterials.2010.01.071

Cited by 399 publications

(323 citation statements)

References 47 publications

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“…48 In two recent studies investigating the adherence of bacteria to medicinally used titanium, the investigators reported that both S. aureus and P. aeruginosa adhered better to the specifically treated and thus on a nanoscale rougher titanium surface than they did to a smooth surface equivalent. 49 Thereby, the observed interspecies differences in adherence could be ascribed to the morphological characteristics of the bacteria. 50 The barbed suture's teeth have two consequences in that respect: An enlarged surface in comparison to monofilament sutures and a macroscopic niche for bacterial growth and possibly biofilm formation.…”

Section: Discussionmentioning

confidence: 99%

Bacterial adhesion to suture material in a contaminated wound model: Comparison of monofilament, braided, and barbed sutures

Dhom

Bloes

Peschel

et al. 2016

Journal Orthopaedic Research

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Contaminated suture material plays an important role in the physiopathology of surgical site infections. Recently, suture material has been developed characterized by barbs projecting from a monofilament base. Claimed advantages for barbed sutures are a shortened wound closure time and reduced maximum wound tension. It has also been suggested that these sutures would be advantageous microbiologically. The aim of this study was to test the microbiological characteristics of the barbed Quill in comparison to the monofilament Ethilon II and the braided sutures Vicryl and triclosan-coated Vicryl Plus. In our study, sutures were cultivated on color-change agar with Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecium, Escherichia coli, and Pseudomonas aeruginosa and the halo size was measured. In a second study arm with longer cultivation bacterial growth was followed by antibiotic treatment. Ethilon II and Quill showed good comparable results, whereas large halos were found around Vicryl. Vicryl Plus results depended on triclosan sensitivity. After longer bacterial cultivation and antibiotic treatment, halos were up to 3.6 times smaller on Quill than on Vicryl (p < 0.001), but 1.4 times larger than on Ethilon II (p < 0.001) regarding S. aureus. Confocal microscopy analysis showed bacterial colonization between the braided filaments on Vicryl and beneath the barbs on Quill. From a microbiological perspective, barbed sutures can be recommended in aseptic surgery, but should only be used carefully in septic surgery.

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Section: Discussionmentioning

confidence: 99%

Bacterial adhesion to suture material in a contaminated wound model: Comparison of monofilament, braided, and barbed sutures

Dhom

Bloes

Peschel

et al. 2016

Journal Orthopaedic Research

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“…roughness) on material surfaces are also crucial in cell and tissue responses to biomaterials. Indeed, surface roughness has shown to alter fibroblast proliferation on aluminiun, titanium and titanium alloys substrates [60][61][62] , and strongly influence bacterial attachment to nitinol, titanium and glass surfaces [63][64][65][66] . Overall, the three functionalization strategies displayed the same value of R a (~ 30 nm; Table 2), slightly increasing the original R a of control samples (~ 25 nm; Table 2).…”

Section: Physicochemical Characterization Of the Surfacesmentioning

confidence: 99%

Antibacterial Properties of hLf1–11 Peptide onto Titanium Surfaces: A Comparison Study Between Silanization and Surface Initiated Polymerization

et al. 2015

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Dental implant failure can be associated to infections which develop into periimplantitis. In order to reduce biofilm formation, several strategies focusing on the use of antimicrobial peptides (AMP) have been studied. To covalently immobilize these molecules onto metallic substrates several techniques have been developed, including silanization and polymer brush prepared by surface-initiated atom transfer radical polymerization (ATRP), with varied peptide binding yield and antibacterial performance. The aim of the present study was to compare the efficiency of these methods to immobilize the lactoferrin-derived hLf1-11 antibacterial peptide onto titanium, and evaluate their antibacterial activity in vitro.Smooth titanium samples were coated with hLf1-11 peptide under three different conditions: silanization with APTES, and polymer brush based coatings with two different silanes. Peptide presence was determined by X-ray photoelectron spectroscopy and the mechanical stability of the coatings was studied under ultrasonication. The LDH assays confirmed that HFFs viability and proliferation were no affected by the treatments. The in vitro antibacterial properties of the modified surfaces were tested with two oral strains (Streptococcus sanguinis and Lactobacillus salivarius) showing an outstanding reduction. A higher decrease in bacterial attachment was noticed when samples were modified by ATRP methods compared to silanization. This effect is likely due to the capacity to immobilize more peptide on the surfaces using polymer brushes and the non-fouling nature of polymer PDMA segment.3

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“…Accelerated stem cell attachment on ECAPed CP-Ti specimens was observed by Estrin [3]. Enhanced bacterials adhesion on nanocrystalline CP-Ti was reported by the paper performed by Truong [4]. Nano size surface roughness produced on the nanocrystalline grained titanium is considered as the mechanisms by them.…”

Section: Introductionmentioning

confidence: 90%

“…The P 2p spectra ( Figure 7a) show P 3+ 2p3/2 at 138.6 eV and P 5+ 2p3/2 at 133.5 eV. CaHPO 4 and Ca 3 (PO 4 ) 2 are possible according to the bonding energy of Ca 2p and P 2p [25]. The semi-quantitative comparison of different types of specimens shown in Figures 6b and 7b indicates larger numbers of depositions containing Ca and P produced on ECAPed and FC specimens than those produced on WQ specimens.…”

Section: Samplementioning

confidence: 98%

“…However its strength can be elevated through microstructural modification by severe plastic deformation (SPD). Not only strength, but also corrosion resistance and surface cells attachment can be improved by the method of SPD [1][2][3][4][5][6][7][8]. The strength increment is correlated to ultrafine grain size given by Hall-Petch relationship.…”

Section: Introductionmentioning

confidence: 99%

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Enhanced Surface Precipitates on Ultrafine-Grained Titanium in Physiological Solution

Zhou

Wang

Zou

2017

Metals

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Enhanced cell adherence to the surface of nanocrystallized commercially pure titanium (CP-Ti) was observed by several authors. However, the understanding of the surface modification of Ti in a physiological solution due to nanocrystallized grain size has not been reached. In this work, equal channel angular pressing (ECAP) was applied to manufacturing ultrafine grained CP-Ti. Martensite and Widmanstatten microstructures were also obtained for comparison. The CP-Ti pieces with different microstructures were subjected to soaking tests in a simulated body fluid. Electrochemical impedance spectroscopy (EIS) measurements, X-ray photoelectron spectroscopy (XPS) and scanning electron microscope (SEM), energy dispersive spectrometer (EDS) were used to characterize the surfaces. The results show the surface precipitates mainly contain Ti, O, Ca and P. The quantity of precipitates on ECAPed CP-Ti is the largest among different specimens corresponded to the observation of the thickest layer formation on ECAPed CP-Ti found by EIS. EDS results show more CaPO and less Ti are included on ECAPed Ti comparing to the deposits on other two types of specimens. Smaller numbers of precipitates and denser film are produced on the surface of the water-quenched CP-Ti. The regeneration kinetics of the CaP precipitates evaluated by Gibbs free energy is introduced to interpret the precipitating behaviors on different CP-Ti specimens.

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The influence of nano-scale surface roughness on bacterial adhesion to ultrafine-grained titanium

Cited by 399 publications

References 47 publications

Bacterial adhesion to suture material in a contaminated wound model: Comparison of monofilament, braided, and barbed sutures

Bacterial adhesion to suture material in a contaminated wound model: Comparison of monofilament, braided, and barbed sutures

Antibacterial Properties of hLf1–11 Peptide onto Titanium Surfaces: A Comparison Study Between Silanization and Surface Initiated Polymerization

Enhanced Surface Precipitates on Ultrafine-Grained Titanium in Physiological Solution

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