The inhibitory effect Polygonum Cillinerve polysaccharide on transmissible gastroenteritis virus of swine

Pan, Xingxue; Zhou, Yu; Duan, Xueqin; Cui, Jing; Liu, Jia; Song, Xiaoping; Ma, Wuren; Zhang, Weiyang; Liu, Yingqiu

doi:10.1016/j.rvsc.2021.08.005

Cited by 14 publications

(6 citation statements)

References 28 publications

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“…Isatis root polysaccharide can inhibit H3N2 swine influenza virus in vivo and in vitro [ 25 ]. Polygonum cillinerve polysaccharide plays antiviral activity via inhibiting transmissible gastroenteritis virus of swine (TGEV) replication, reducing TGEV-mediated apoptosis and ROS level [ 26 ]. Therefore, plant polysaccharides could be a potential source of antiviral medicines.…”

Section: Discussionmentioning

confidence: 99%

Antiviral Activity of Plantago asiatica Polysaccharide against Pseudorabies Virus In Vitro

Huan

Zhang

et al. 2022

Oxidative Medicine and Cellular Longevity

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Pseudorabies (PR) is an acute infectious disease of various domestic animals and wild animals caused by pseudorabies virus (PRV). It is mainly characterized by fever, itching, encephalomyelitis, and respiratory and neurological disorders. Plantago asiatica polysaccharide (PLP), extracted from the whole plant of Plantago asiatica L., showed immunomodulatory and antioxidation effects, but the antiviral activity had not been reported. In this study, the inhibitory effect of PLP on PRV infection was studied. Our study first revealed that PLP could inhibit PRV infection in a dose-dependent manner. By adding PLP at different stages of the virus’s life cycle, we revealed that PLP could reduce the attachment and penetration of PRV into PK15 cells. The inhibition of PRV attachment was better than inhibition of PRV penetration. However, PLP did not affect PRV replication and inactivation. In addition, PLP decreased the intracellular ROS levels in infected cells significantly, and ROS scavenger NAC decreased PRV infection. Therefore, our study provided preliminary data of anti-PRV activity of PLP, which was established to be a novel anti-PRV infection agent.

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Section: Discussionmentioning

confidence: 99%

Antiviral Activity of Plantago asiatica Polysaccharide against Pseudorabies Virus In Vitro

Huan

Zhang

et al. 2022

Oxidative Medicine and Cellular Longevity

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“…(+)-Catechin, a catechin in green tea, has anti-TGEV properties, which are related to its antioxidation [ 57 ]. Polygonum Cillinerve polysaccharide can inhibit TGEV replication and alleviate ROS expression and apoptosis [ 58 ]. Tyrphostin A9 is a receptor tyrosine kinase inhibitor of the tyrphostin class.…”

Section: Bioactive Compounds With Anti-pecov Activitymentioning

confidence: 99%

A Review of Bioactive Compounds against Porcine Enteric Coronaviruses

Duan

Luo

Liang

et al. 2022

Viruses

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Pig diarrhea is a universal problem in the process of pig breeding, which seriously affects the development of the pig industry. Porcine enteric coronaviruses (PECoVs) are common pathogens causing diarrhea in pigs, currently including transmissible gastroenteritis virus (TGEV), porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV) and swine acute diarrhea syndrome coronavirus (SADS-CoV). With the prosperity of world transportation and trade, the spread of viruses is becoming wider and faster, making it even more necessary to prevent PECoVs. In this paper, the host factors required for the efficient replication of these CoVs and the compounds that exhibit inhibitory effects on them were summarized to promote the development of drugs against PECoVs. This study will be also helpful in discovering general host factors that affect the replication of CoVs and provide references for the prevention and treatment of other CoVs.

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“…Previous research has revealed that PCP was primarily composed of glucose, it was α-d-glucan and the backbone was consisted of repeating units of (1 → 4)-α-d-glucose ( 17 ). In addition, research has also shown that Polygonum Cillinerve polysaccharide could significantly decrease the cell apoptosis rate and the expression of ROS induced by TGEV, reduce TGEV replication, increase the expression levels of Bcl-2 and Bax mRNA, increase the expression of Bcl-2, reduce cyt C protein, and inhibit caspase 3 degradation ( 18 ).…”

Section: Introductionmentioning

confidence: 99%

Effect of miR-17 on Polygonum Cillinerve polysaccharide against transmissible gastroenteritis virus

Duan,

Xu,

Wang

et al. 2024

Front. Vet. Sci.

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Transmissible gastroenteritis virus (TGEV) could cause diarrhea, vomiting, dehydration and even death in piglets, miRNA played an important role in the interaction between virus and cell. The study aimed to investigate the impact of miR-17 on the polysaccharide of Polygonum Cillinerve (PCP) in combating TGEV. miR-17 was screened and transfection validation was performed by Real-time PCR. The function of miR-17 on PK15 cells infected with TGEV and treated with PCP was investigated by DCFH-DA loading probe, JC-1 staining and Hoechst fluorescence staining. Furthermore, the effect of miR-17 on PCP inhibiting TGEV replication and apoptosis signaling pathways during PCP against TGEV infection was measured through Real-time PCR and Western blot. The results showed that miR-17 mimic and inhibitor could be transferred into PK15 cells and the expression of miR-17 significantly increased and decreased respectively compared with miR-17 mimic and inhibitor (P < 0.05). A total 250 μg/mL of PCP could inhibit cells apoptosis after transfection with miR-17. PCP (250 μg/mL and 125 μg/mL) significantly inhibited the decrease in mitochondrial membrane potential induced by TGEV after transfection with miR-17 (P < 0.05). After transfection of miR-17 mimic, PCP at concentrations of 250 μg/mL and 125 μg/mL significantly promoted the mRNA expression of P53, cyt C and caspase 9 (P < 0.05). Compared with the control group, the replication of TGEV gRNA and gene N was significantly inhibited by PCP at concentrations of 250 μg/mL and 125 μg/mL after transfection of both miR-17 mimic and inhibitor (P < 0.05). PCP at 62.5 μg/mL significantly inhibited the replication of gene S following transfection with miR-17 inhibitor (P < 0.05). These results suggested that PCP could inhibit the replication of TGEV and apoptosis induced by TGEV by regulating miR-17.

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The inhibitory effect Polygonum Cillinerve polysaccharide on transmissible gastroenteritis virus of swine

Cited by 14 publications

References 28 publications

Antiviral Activity of Plantago asiatica Polysaccharide against Pseudorabies Virus In Vitro

Antiviral Activity of Plantago asiatica Polysaccharide against Pseudorabies Virus In Vitro

A Review of Bioactive Compounds against Porcine Enteric Coronaviruses

Effect of miR-17 on Polygonum Cillinerve polysaccharide against transmissible gastroenteritis virus

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