The interaction between Histoplasma capsulatum cell wall carbohydrates and host components: relevance in the immunomodulatory role of histoplasmosis

Gorocica, Patrícia; Taylor, Mária Lucía; Alvarado-Vásquez, Noé; Pérez‐Torres, Armando; Lascuraín, Ricardo; Zenteno, Edgar

doi:10.1590/s0074-02762009000300016

Cited by 20 publications

(12 citation statements)

References 49 publications

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“…A similar composition has been previously reported for the cell wall of other species of pathogenic fungi, such as Histoplasma capsulatum (Gorocica et al, 2009). However, for Aspergillus fumigatus, A. wentii and Chaetosartory chrysella , mannose-containing molecules were also found forming cell walls as galactomannans with distinct chemical and physical properties (Gomez-Miranda et al, 2003).…”

Section: Resultssupporting

confidence: 86%

Glycan analysis of Fonsecaea monophora from clinical and environmental origins reveals different structural profile and human antigenic response

Burjack

Santana-Filho

Ruthes

et al. 2014

Front. Cell. Infect. Microbiol.

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Dematiaceous fungi constitute a large and heterogeneous group, characterized by having a dark pigment, the dihydroxynaftalen melanin—DHN, inside their cell walls. In nature they are found mainly as soil microbiota or decomposing organic matter, and are spread in tropical and subtropical regions. The fungus Fonsecaea monophora causes chromoblastomycosis in humans, and possesses essential mechanisms that may enhance pathogenicity, proliferation and dissemination inside the host. Glycoconjugates confer important properties to these pathogenic microorganisms. In this work, structural characterization of glycan structures present in two different strains of F. monophora MMHC82 and FE5p4, from clinical and environmental origins, respectively, was performed. Each one were grown on Minimal Medium (MM) and Czapeck-Dox (CD) medium, and the water soluble cell wall glycoconjugates and exopolysaccharides (EPS) were evaluated by NMR, methylation and principal component analysis (PCA). By combining the methylation and 2D NMR analyses, it was possible to visualize the glycosidic profiles of the complex carbohydrate mixtures. Significant differences were observed in β-D-Galf-(1→5) and (1→6) linkages, α- and β-D-Glcp-(1→3), (1→4), and (1→6) units, as well as in α-D-Manp. PCA from 1H-NMR data showed that MMHC82 from CD medium showed a higher variation in the cell wall carbohydrates, mainly related to O-2 substituted β-D-Galf (δ 106.0/5.23 and δ 105.3/5.23) units. In order to investigate the antigenic response of the glycoconjugates, these were screened against serum from chromoblastomycosis patients. The antigen which contained the cell wall of MMHC82 grown in MM had β-D-Manp units that promoted higher antigenic response. The distribution of these fungal species in nature and the knowledge of how cell wall polysaccharides and glycoconjugates structure vary, may contribute to the better understanding and the elucidation of the pathology caused by this fungus.

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Section: Resultssupporting

confidence: 86%

Glycan analysis of Fonsecaea monophora from clinical and environmental origins reveals different structural profile and human antigenic response

Burjack

Santana-Filho

Ruthes

et al. 2014

Front. Cell. Infect. Microbiol.

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“…The latter is the predominant component of the fungus in the mycelial stage, participates in leukocyte recruitment and stimulates the production of inflammatory mediators. 57 b-glucans interact with dectin-1, a C-type lectin. Dectin-1 is an important b-glucan receptor and one of the first PRRs identified that can mediate signaling both independently and synergistically with TLRs to initiate specific responses to microorganisms.…”

Section: Dendritic Cells In the Immune Responsementioning

confidence: 99%

“…Dectin-1 is found in neutrophils, natural killer cells, DCs and a subset of T cells. 57 Dectin-1 engagement is associated with enhanced phagocytosis, 58 reactive oxygen species production 59 and up-regulation of cytokine production. 60 a-1,3-glucan contributes to virulence in Histoplasma as avirulent strains produce significantly less a-1,3-glucan.…”

Section: Dendritic Cells In the Immune Responsementioning

confidence: 99%

Dendritic cell interactions withHistoplasmaandParacoccidioides

2015

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“…Lung histoplasmosis is histoplasma capsulatum infection caused by a type of fungal disease, the bacteria present in the soil is mainly a bird or bat droppings or dust contaminated, is a bipolar fungi within the organization and 37 ℃ medium was yeast type, showing hyphae at room temperature and soil type, the size of the spores [5][6][7]. Fungal spores inhaled into the lungs after the macrophages and other phagosome swallowed, by converting to a yeast form and avoid being cleared.…”

Section: Discussionmentioning

confidence: 99%

Differential Diagnosis Value of 18F-FDG Imaging in Lung Histoplasmosis and Lung Cancer

He¹,

Wu²,

Huang³

et al. 2016

JPP

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Abstract:In order to confirm the accuracy of ECT (emission computed tomography) scanning imaging in the diagnosis of lung histoplasmosis is higher than CT (computed tomography) scan. We retrospect a lung histoplasmosis patient, he was examined by the CT scan and ECT scanning, respectively. Results showed that lung cancer for CT diagnosis and large benign lesions for 18 F-FDG ( 18 F-fluorodeoxyglucose) ECT diagnosis. This indicated that the ECT diagnosis for benign lesions in the lung maybe have higher accuracy than CT scan.

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The interaction between Histoplasma capsulatum cell wall carbohydrates and host components: relevance in the immunomodulatory role of histoplasmosis

Cited by 20 publications

References 49 publications

Glycan analysis of Fonsecaea monophora from clinical and environmental origins reveals different structural profile and human antigenic response

Glycan analysis of Fonsecaea monophora from clinical and environmental origins reveals different structural profile and human antigenic response

Dendritic cell interactions withHistoplasmaandParacoccidioides

Differential Diagnosis Value of 18F-FDG Imaging in Lung Histoplasmosis and Lung Cancer

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