The intercellular adhesin locus ica is present in clinical isolates of Staphylococcus aureus from bacteremic patients with infected and uninfected prosthetic joints

Fowler, Vance G.; Fey, Paul D.; Reller, L. Barth; Chamis, Anna Lisa; Corey, G. Ralph; Rupp, Mark E.

doi:10.1007/s430-001-8018-5

Cited by 66 publications

(44 citation statements)

References 12 publications

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“…S. aureus comprised the majority (56% [55 out of 99]) of the PJI-associated isolates investigated in this study, and all were found to be icaA positive, substantiating past findings that the ica operon is common to all strains of S. aureus (13,14,22). A total of 55% (17 out of 31) of S. epidermidis PJI isolates were icaA positive.…”

supporting

confidence: 90%

icaA Is Not a Useful Diagnostic Marker for Prosthetic Joint Infection

Frank¹,

Hanssen

Patel

2004

J Clin Microbiol

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A collection of 99 staphylococcal isolates associated with prosthetic joint infection and 23 coagulase-negative staphylococci isolated from noninfected arthroplasty-associated specimens were screened in order to determine whether the presence of icaA could be used to distinguish between pathogens and nonpathogens. All Staphylococcus aureus prosthetic joint infection isolates (n ‫؍‬ 55) were icaA positive. A total of 46% (20 out of 44) of coagulase-negative staphylococcal prosthetic joint infection isolates were icaA positive, and 30% (7 out of 23) of arthroplasty-associated non-prosthetic joint infection-associated coagulase-negative staphylococcal isolates were icaA positive (P ‫؍‬ 0.23). Certain coagulase-negative Staphylococcus species appeared more likely to be isolated as either arthroplasty-associated non-prosthetic joint infection-associated isolates (e.g., Staphylococcus warneri and Staphylococcus hominis) or pathogens (e.g., Staphylococcus lugdunensis). The presence of icaA in a coagulase-negative staphylococcal isolate associated with an arthroplasty is not a useful diagnostic indicator of pathogenicity.

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supporting

confidence: 90%

icaA Is Not a Useful Diagnostic Marker for Prosthetic Joint Infection

Frank¹,

Hanssen

Patel

2004

J Clin Microbiol

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“…Among the group of 102 S. aureus mastitis isolates from India, only 36 revealed the presence of both genes (Dhanawade et al, 2010). On the other hand, several authors showed presence of the ica locus genes in all S. aureus clinical isolates analyzed in their studies (Fowler et al, 2001;Rohde et al, 2001;Knobloch et al, 2002;Asthan andShamsudin, 2011, Szweda et al, 2012). These variations could be due to circulations of different clones of S. aureus in different regions.…”

Section: Discussionmentioning

confidence: 98%

Genotypic and Phenotypic Characterization of Clinical Isolates of Staphylococcus Aureus for Biofilm Formation Ability

Goyal¹

2014

Adv. Anim. Vet. Sci.

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The objective of the study was to characterize Staphylococcus aureus (S. aureus) isolated from human and animal clinical cases for their biofilm formation ability by genotypic and phenotypic methods. A total of 130 S. aureus strains isolated from human wound (n = 20), animal wound (n = 70) and animal mastitis (n = 40) cases were subjected to screening for 3 different biofilm associated genes (bap, icaA and icaD) and for phenotypic assessment for biofilm formation using Congo red agar, modified Congo red agar and microtitre plate assay. PCR assays were standardized for the detection of bap, icaA and icaD genes. The results indicated that icaA gene was present in 51.15% of the isolates and bap gene was present in 8.46% isolates. None of the isolates were positive for icaD gene. Human isolates (65%) had higher occurrence of icaA gene in comparison to animal isolates (49.09%). Dog wound isolates had higher occurrence of bap gene. Of the 3 methods used for phenotypic expression of biofilm by S. aureus isolates modified Congo red agar method showed 86.92% isolates to be positive, whereas by Congo red agar method only 63.07% S. aureus were found to be biofilm producer. Microtitre plate assay showed 75.38% S. aureus isolates to be biofilm producers. A good correlation was observed between genotypic and phenotypic biofilm formation ability of the isolates. Bap gene contained isolates showed higher biofilm producing ability compare to icaA gene harbored isolates.All copyrights reserved to Nexus® academic publishers

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“…A presença do gene icaD foi observada em dois S. aureus bubalino e três S. intermedius (2 bovinos e 1 bubalino). Arciola et al (2001) detectaram o gene icaD em 61,0% dos isolados, enquanto Fowler et al (2001) encontraram o mesmo gene em todas as amostras pesquisadas. Neste trabalho, nenhuma amostra foi positiva para o gene icaD e CRA simultaneamente e quatro amostras foram positivas para icaD e o teste de Aderência em Placa.…”

Section: Discussão E Conclusõesunclassified

Caracterização fenotípica, produção de biofilme e resistência aos antimicrobianos em isolados de Staphylococcus spp. obtidos de casos de mastite em bovinos e bubalinos

et al. 2012

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No presente estudo, objetivou-se avaliar a sussuscetibilidade aos principais antimicrobianos e realizar uma caracterização fenotípica e genotípica de isolados de Staphylococcus spp. obtidos de casos de mastite em vacas (n=30) e búfalas (n=30). A suscetibilidade foi avaliada pela técnica de disco-difusão e a presença de bomba de efluxo foi avaliada utilizando-se Ágar Mueller Hinton (MH) adicionado de brometo de etídeo e pesquisa do gene msrA. Pela técnica da Reação em Cadeia da Polimerase (PCR) ainda foram identificados os genes mecA, blaZ e ermA, B e C, que posteriormente foram associados com os métodos fenotípicos para a identificação de resistência a antimicrobianos. A caracterização da formação de biofilme foi realizada utilizandose os métodos Ágar Vermelho Congo (CRA), Aderência em Placa e a identificação do gene icaD. Pelo método de discodifusão, os Staphylococcus spp. apresentaram alta sensibilidade aos antimicrobianos. O índice de resistência múltipla aos antimicrobianos (IRMA) apresentou variação de 0 a 0,5. Na pesquisa de bomba de efluxo, 26,7% das amostras foram positivas ao método fenotípico e 6,7% ao método genotípico (gene msrA). Os genes erm, mecA e blaZ foram detectados, respectivamente, em 1,7%, 6,7% e 11,7% das amostras de Staphylococcus spp. Na produção de biofilme, 23,3% dos isolados foram considerados positivos no CRA, 50,0% na Aderência em Placas e 8,3% na PCR pela detecção do gene icaD. Observou-se que os isolados obtidos de amostras bovinas apresentaram uma menor sensibilidade aos antimicrobianos no teste de disco-difusão quando comparados com as amostras bubalinas. A caracterização destes isolados é importante para orientar uma antibioticoterapia bem planejada. A presença de biofilme nos isolados pode estar associada a outros fatores que não a resistência às drogas antimicrobianas.

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The intercellular adhesin locus ica is present in clinical isolates of Staphylococcus aureus from bacteremic patients with infected and uninfected prosthetic joints

Cited by 66 publications

References 12 publications

icaA Is Not a Useful Diagnostic Marker for Prosthetic Joint Infection

icaA Is Not a Useful Diagnostic Marker for Prosthetic Joint Infection

Genotypic and Phenotypic Characterization of Clinical Isolates of Staphylococcus Aureus for Biofilm Formation Ability

Caracterização fenotípica, produção de biofilme e resistência aos antimicrobianos em isolados de Staphylococcus spp. obtidos de casos de mastite em bovinos e bubalinos

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