The intercorrelation among CCT6A, CDC20, CCNB1, and PLK1 expressions and their clinical value in papillary thyroid carcinoma prognostication

Abstract

Background CCT6A promotes several carcinomas' growth and invasion in multiple ways, and it relates to CCNB1 and PLK1 through its interaction with CDC20 via protein–protein interaction bioinformatics. This study aimed to explore the intercorrelation among CCT6A, CDC20, CCNB1, and PLK1, and their association with tumor features and prognosis in papillary thyroid carcinoma (PTC) patients. Methods CCT6A, CDC20, CCNB1, and PLK1 expressions in 186 tumor and 30 non‐tumor specimens from PTC patients were determined by immunohistochemical (IHC). Clinical features, disease‐free survival (DFS), and overall survival (OS) were retrieved. Results CCT6A, CDC20, CCNB1, and PLK1 expressions were upregulated in tumor tissues compared with non‐tumor tissues (all p < 0.001). CCT6A expression positively correlated with CDC20, CCNB1, and PLK1 expressions; besides, CDC20 expression positively associated with CCNB1 and PLK1 expressions, and CCNB1 expression was also positively related to PLK1 expression (all p < 0.05). Moreover, elevated tumor CCT6A expression was correlated with extrathyroidal invasion ( p = 0.015), higher pT stage ( p < 0.001), pN stage ( p = 0.046), and pTNM stage ( p = 0.042); while tumor CDC20, CCNB1, and PLK1 expressions only correlated with some of these indexes (most p < 0.05). Notably, CCT6A and CDC20 high expressions predicted worse DFS and OS (all p < 0.05); CCNB1 positive expression only predicted poor DFS ( p = 0.044) but not OS ( p = 0.152); however, PLK1 expression failed to predict these two indexes (both p > 0.05). After adjustment using multivariate Cox's regression, CCT6A expression (high vs. low) independently estimated shorter DFS ( p = 0.010) and OS ( p = 0.006). Conclusion CCT6A, CDC20, CCNB1, and PLK1 are intercorrelated, and they exhibit certain prognostic values in PTC patients.

CCT6A is associated with CDC20, Enneking stage and prognosis in osteosarcoma, and its knockdown suppresses osteosarcoma cell viability and invasion

“…CCT6A and CDC20 are overexpressed in various types of cancer (16,27,(29)(30)(31)(32), and a previous study reported that CCT6A is positively associated with CDC20 in patients with papillary thyroid carcinoma (16). However, the dysregulation and intercorrelation of CCT6A and CDC20 in patients with osteosarcoma still needs to be explored.…”

“…Chaperonin-containing tailless complex polypeptide 1 subunit 6A (CCT6A) is a recently discovered oncogene, which promotes cancer progression by facilitating the proliferation, invasiveness and stemness and of tumor cells (10)(11)(12)(13). In addition, it exhibits potency as a prognostic marker in numerous types of cancer, such as lung cancer, gastric cancer and papillary thyroid carcinoma (14)(15)(16). Notably, CCT6A interacts with cell division cycle 20 (CDC20), as shown by the STRING database (https://cn.string-db.org/) and further validated by a previous study in papillary thyroid carcinoma cancer tissue (16).…”

“…In addition, it exhibits potency as a prognostic marker in numerous types of cancer, such as lung cancer, gastric cancer and papillary thyroid carcinoma ( 14 – 16 ). Notably, CCT6A interacts with cell division cycle 20 (CDC20), as shown by the STRING database ( ) and further validated by a previous study in papillary thyroid carcinoma cancer tissue ( 16 ). CDC20 is a well-known oncogene that facilitates the initiation and progression of several types of cancer, including osteosarcoma ( 17 – 21 ).…”

“…Therefore, it was hypothesized that CCT6A may be involved in the pathogenesis and prognosis of osteosarcoma. Furthermore, previous studies have reported that CCT6A regulates osteosarcoma cell proliferation via Akt activation ( 22 ) and that CCT6A is associated with CDC20 in patients with papillary thyroid carcinoma ( 16 ). However, these studies did not explore the association between CCT6A and clinical/pathological features, neoadjuvant chemotherapy response and prognosis in patients with osteosarcoma, nor did the assess the relationship between CCT6A and CDC20 in patients with osteosarcoma.…”

Increased expression of GIPC2 in colon adenocarcinoma is associated with a favorable prognosis and high levels of immune cell infiltration

“…Two senior pathologists performed double-blind evaluations by examining the sections under a light microscope. According to a previous study (16), 10 high-power fields were randomly selected from each section. A semi-quantitative score based on the intensity of positively stained cells and the staining area was used to evaluate the results of IHC; the comprehensive score of staining intensity was multiplied by the staining area.…”