The International Mycotoxin Check Sample Programme

Friesen, Marlin D.

doi:10.3109/15569548909059766

“…This could be due to the use of TLC which is know n to be less speci® c than H PLC or ELISA m ethods for the analysis of traces of a¯atoxin M 1 (i.e. under the mg/ kg-level) and often leads to w idespread results in the a¯atoxin M 1 content in samples (Friesen 1989). The only labora tory using an ELISA test obtaine d`questionable' z-scores precisely located on the line between acceptable and questionable scores.…”

Section: Discussionmentioning

confidence: 97%

Proficiency testing for the evaluation of the ability of European Union-National Reference laboratories to determine aflatoxin M1 in milk at levels corresponding to the new European Union legislation

Dragacci¹,

Grosso²,

Pfauwathel-Marchond³

et al. 2001

Food Add. Contaminants

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In 1992, the European Union set up a network of National Reference Laboratories and charged the Community Reference Laboratory with the responsibility to design a proficiency testing scheme for assessing the analytical ability of laboratories involved in the official control of aflatoxin M1 in milk. Since 1996, two exercises of proficiency testing have been performed on samples of milk powder and liquid milk at various levels of aflatoxin M1 contents. The trials were conducted according to ISO Guide 43, in particular for the homogeneity testing of sample batches and for the calculation of laboratory z-scores. The National Reference Laboratories officially designated by their governments participated in this programme. Samples were naturally-contaminated milk obtained by feeding cows with aflatoxin B1-contaminated feed. The levels of aflatoxin M1 in the samples ranged from 0.2 to 0.7 microg/kg in milk powder and from 0.05 to 0.07 microg/l in liquid milk. These levels were chosen as being close to the European Union-regulated limit of 0.05 microg of aflatoxin M1 per litre. The results produced by laboratories were compiled and statistically analysed to detect any outlying results and to calculate the individual z-scores. Except for one laboratory in each exercise, all laboratories exhibited acceptable or questionable z-scores. The interlaboratory relative standard deviation for reproducibility (RSDR) obtained for both 1996 and 1998 exercises were in the range 15.7-30.3%. Compared with other published studies, this indicates a very good precision for the performance of this laboratory network in the analysis of traces of aflatoxin M1 in milk.

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“…This could be due to the use of TLC which is known to be less speci® c than H PLC or ELISA m ethods for the analysis of traces of a¯atoxin M 1 (i.e. under the mg/ kg-level) and often leads to w idespread results in the a¯atoxin M 1 content in samples (Friesen 1989). The only labora tory using an ELISA test obtaine d`questionable' z-scores precisely located on the line between acceptable and question able scores.…”

Section: Discussionmentioning

confidence: 97%

Proficiency testing for the evaluation of the ability of European Union-National Reference laboratories to determine aflatoxin M₁in milk at levels corresponding to the new European Union legislation

Dragacci

¹

,

Grosso

²

,

Pfauwathel-Marchond

³

et al. 2001

Food Additives and Contaminants

9

0

View full text Add to dashboard Cite

In 1992, the European Union set up a network of National Reference Laboratories and charged the Community Reference Laboratory with the responsibility to design a proficiency testing scheme for assessing the analytical ability of laboratories involved in the official control of aflatoxin M1 in milk. Since 1996, two exercises of proficiency testing have been performed on samples of milk powder and liquid milk at various levels of aflatoxin M1 contents. The trials were conducted according to ISO Guide 43, in particular for the homogeneity testing of sample batches and for the calculation of laboratory z-scores. The National Reference Laboratories officially designated by their governments participated in this programme. Samples were naturally-contaminated milk obtained by feeding cows with aflatoxin B1-contaminated feed. The levels of aflatoxin M1 in the samples ranged from 0.2 to 0.7 microg/kg in milk powder and from 0.05 to 0.07 microg/l in liquid milk. These levels were chosen as being close to the European Union-regulated limit of 0.05 microg of aflatoxin M1 per litre. The results produced by laboratories were compiled and statistically analysed to detect any outlying results and to calculate the individual z-scores. Except for one laboratory in each exercise, all laboratories exhibited acceptable or questionable z-scores. The interlaboratory relative standard deviation for reproducibility (RSDR) obtained for both 1996 and 1998 exercises were in the range 15.7-30.3%. Compared with other published studies, this indicates a very good precision for the performance of this laboratory network in the analysis of traces of aflatoxin M1 in milk.

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Chapter 6 Emerging Techniques: Enzyme-Linked Immunosorbent Assay (ELISA) as Alternatives to Chromatographic Methods

Ward

¹

,

Wilkinson

²

,

Morgan

³

1993

Chromatography of Mycotoxins - Techniques and Applications

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The International Mycotoxin Check Sample Programme

Cited by 3 publications

References 2 publications

Proficiency testing for the evaluation of the ability of European Union-National Reference laboratories to determine aflatoxin M1 in milk at levels corresponding to the new European Union legislation

Proficiency testing for the evaluation of the ability of European Union-National Reference laboratories to determine aflatoxin M1 in milk at levels corresponding to the new European Union legislation

Proficiency testing for the evaluation of the ability of European Union-National Reference laboratories to determine aflatoxin M₁in milk at levels corresponding to the new European Union legislation

Chapter 6 Emerging Techniques: Enzyme-Linked Immunosorbent Assay (ELISA) as Alternatives to Chromatographic Methods

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The International Mycotoxin Check Sample Programme

Cited by 3 publications

References 2 publications

Proficiency testing for the evaluation of the ability of European Union-National Reference laboratories to determine aflatoxin M1 in milk at levels corresponding to the new European Union legislation

Proficiency testing for the evaluation of the ability of European Union-National Reference laboratories to determine aflatoxin M1 in milk at levels corresponding to the new European Union legislation

Proficiency testing for the evaluation of the ability of European Union-National Reference laboratories to determine aflatoxin M1in milk at levels corresponding to the new European Union legislation

Chapter 6 Emerging Techniques: Enzyme-Linked Immunosorbent Assay (ELISA) as Alternatives to Chromatographic Methods

Contact Info

Product

Resources

About

Proficiency testing for the evaluation of the ability of European Union-National Reference laboratories to determine aflatoxin M₁in milk at levels corresponding to the new European Union legislation