“…Of 3 measurements made for each participant the highest value was used for this study. The main study factors were as follows: (a) physical exercise, assessed by the International Physical Activity Questionnaire and daily hours walked outdoors (www.ipaq.ki.se) [13]; (b) nutritional status, assessed by anthropometric measurements (weight, height, body mass index), recent weight loss and the short-form Mini Nutritional Assessment questionnaire; (c) comorbidities (arthrosis, diabetes, ischaemic heart disease, heart failure, stroke, chronic obstructive pulmonary disease, chronic kidney failure, chronic liver disease, Parkinson disease, depression, etc); (d) anabolic hormone levels, namely, fasting plasma levels of total ghrelin, insulin-like growth factor 1 (IGF-1), testosterone and insulin determined using validated commercial kits; (e) inflammatory markers, namely, fasting plasma levels of interleukin-6 (IL-6) and C-reactive protein, determined using validated commercial kits; and (f) frailty phenotype, whereby participants were classified as robust, pre-frail or frail if they fulfilled 0, 1-2 or ≥3, respectively, of the following five Fried criteria: unintentional weight loss, exhaustion, low physical activity, slow walking speed and poor grip strength [5]. Other study variables included sociodemographic characteristics (age, sex, education level); chronic medication; appetite and satiety assessed by means of a visual analogue scale; functional capacity assessed by the Barthel index, timed up-and-go test, single-leg stance test, falls and gait speed; and, finally, a complete blood count and basic blood biochemical analyses for glucose, creatinine, albumin and lipid profile.…”