The interplay between miR156/SPL13 and DFR/WD40–1 regulate drought tolerance in alfalfa

Feyissa, Biruk A.; Arshad, Muhammad; Gruber, Margaret Y.; Kohalmi, Susanne E.; Hannoufa, Abdelali

doi:10.1186/s12870-019-2059-5

Cited by 108 publications

(126 citation statements)

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“…A previous report indicated a direct binding of SPL13 to DFR promoter (Feyissa et al, 2019) to enhance anthocyanin biosynthesis. Using the current IPMS analysis, we were able to identify DFR and other candidate proteins interacting with SPL13, such as phenylalanine ammonia-lyase1(PAL1) which catalyses the rst step in the phenylpropanoid pathway by converting phenylalanine to cinnamate (Nuñez-Palenius and Ochoa-Alejo, 2005).…”

Section: Spl13 Interacts With Photosynthesis-and Phenylpropanoid Pathmentioning

confidence: 91%

Section: Photosynthesis-related Deg Are Upregulated In Leaves Of Spl1mentioning

confidence: 97%

“…Physiological investigation of SPL13RNAi plants under drought stress showed maintenance of vital physiological processes, such as photosynthesis and leaf water holding capacity (Feyissa et al, 2019). This was accompanied by an upregulation of photosynthesis-related genes (PSI and PSII) in SPL13RNAi plants (Feyissa et al, 2019). In the current study, comparison of transcript pro les of leaves of both genotypes under control and drought conditions revealed that various metabolic pathways were affected, with photosynthesis being predominantly higher in SPL13RNAi plants.…”

Section: Photosynthesis-related Deg Are Upregulated In Leaves Of Spl1mentioning

confidence: 97%

“…Among the molecular strategies, the role of microRNAs in regulating various plant processes to enhance stress tolerance has been reported in a variety of crops (Hannoufa et al, 2018). Of the hundreds of plant microRNAs, microRNA156 (miR156) is Previous reports showed that the miR156/SPL13 module regulates physiological, metabolic and phenotypic adjustments in alfalfa under drought (Arshad et al, 2017;Feyissa et al, 2019). To understand SPL13-driven drought stress tolerance strategy, the global transcriptomic pro le of leaf, stem, and root tissues of RNAi-silenced SPL13 and EV plants were investigated under drought stress.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome-IPMS analysis reveals a tissue-dependent miR156/SPL13 regulatory mechanism in alfalfa drought tolerance

Feyissa

Renaud

Nasrollahi

et al. 2020

Preprint

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Background We previously reported on the interplay between miR156/SPL13 and WD40-1/DFR to improve response to drought stress in alfalfa (Medicago sativa L.). Here we aimed to investigate whether the role of miR156/SPL13 module in drought response is tissue-specific, and to identify SPL13-interacting proteins. We analyzed the global transcript profiles of leaf, stem, and root tissues of one-month old RNAi-silenced SPL13 (SPL13RNAi) alfalfa plants exposed to drought stress and conducted protein-protein interaction analysis to identify SPL13 interacting partners . Result Transcript analysis combined with weighted gene co-expression network analysis showed tissue and genotype-specific gene expression patterns. Moreover, pathway analysis of stem-derived differentially expressed genes (DEG) revealed upregulation of genes associated with stress mitigating primary and specialized metabolites, whereas genes associated with photosynthesis light reactions were silenced in SPL13RNAi plants. Leaf-derived DEG were attributed to enhanced light reactions, largely photosystem I, II, and electron transport chains, while roots of SPL13RNAi plants upregulated transcripts associated with metal ion transport, carbohydrate, and primary metabolism. Using immunoprecipitation combined with mass spectrometry (IPMS) we showed that SPL13 interacts with proteins involved in photosynthesis, specialized metabolite biosynthesis, and stress tolerance. Conclusions We conclude that the miR156/SPL13 module mitigates drought stress in alfalfa by regulating molecular and physiological processes in a tissue-dependent manner.

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Section: Spl13 Interacts With Photosynthesis-and Phenylpropanoid Pathmentioning

confidence: 91%

Section: Photosynthesis-related Deg Are Upregulated In Leaves Of Spl1mentioning

confidence: 97%

Section: Photosynthesis-related Deg Are Upregulated In Leaves Of Spl1mentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Transcriptome-IPMS analysis reveals a tissue-dependent miR156/SPL13 regulatory mechanism in alfalfa drought tolerance

Feyissa

Renaud

Nasrollahi

et al. 2020

Preprint

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“…Previously, our group showed that overexpression of miR156 improved multiple physiological traits and altered the transcriptome pro le of alfalfa [30]. Further investigations revealed a positive role for miR156 in abiotic stress tolerance, including drought [26,31], salinity [27] and heat [28]. In addition, transcriptomic analysis showed that miR156 affects a wide array of gene families under drought stress in alfalfa [23].…”

Section: Alfalfa Proteome Is Affected By Mir156 Under Heat Stressmentioning

confidence: 98%

Label-free quantitative proteomic analysis of alfalfa in response to microRNA156 under high temperature

Arshad

Puri

Simkovich

et al. 2020

Preprint

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Background Abiotic stress, including heat, is one of the major factors that affect alfalfa growth and forage yield. The small RNA, microRNA156 (miR156), regulates multiple traits in alfalfa during abiotic stress. The aim of this study was to explore the role of miR156 in regulating heat response in alfalfa at the protein level. Results In this study, we compared an empty vector control and miR156 overexpressing (miR156OE) alfalfa plants after exposing them to heat stress (40 ° C) for 24 hours. We measured physiological parameters of control and miR156OE plants under heat stress, and collected leaf samples for protein analysis. A higher proline and antioxidant contents were detected in miR156OE plants than in controls under heat stress. Protein samples were analyzed by label-free quantification proteomics. Across all samples, a total of 1878 protein groups were detected. Under heat stress, 45 protein groups in the empty vector plants were significantly altered (P < 0.05; |log 2 FC| > 2). Conversely, 105 protein groups were significantly altered when miR156OE alfalfa was subjected to heat stress, of which 91 were unique to miR156OE plants. The identified protein groups unique to miR156OE plants were related to diverse functions including metabolism, photosynthesis, stress-response and plant defenses. Furthermore, we identified transcription factors in miR156OE plants, which belonged to squamosa promoter binding-like protein, MYB, ethylene responsive factors, AP2 domain, ABA response element binding factor and bZIP families of transcription factors. Conclusions These results suggest a positive role for miR156 in heat stress response in alfalfa. They reveal a miR156-regulated network of mechanisms at the protein level to modulate heat responses in alfalfa.

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Analysis of the transcriptome and metabolome reveals phenylpropanoid mechanism in common bean (Phaseolus vulgaris) responding to salt stress at sprout stage

Zhang,

Wang,

Qin

et al. 2023

Food and Energy Security

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Common bean (Phaseolus vulgaris) seeds are important legume crops and an important source of dietary proteins and carbohydrates. Therefore, it is important to develop strategies to improve salt tolerance in common beans. In this study, transcriptome and metabolome analyses were conducted on local common bean variety under salt stress at the sprout stage for a period of 0, 12, and 24 h. Results showed that phenylpropanoid pathways (including phenylpropanoid biosynthesis and phenylalanine metabolism) and flavonoid pathways (including flavonoid biosynthesis and flavone and flavonol biosynthesis) played an important role in controlling responses to salt stress as evidenced by analysis of differentially expression genes, common expression patterns, WCGNA, and differentially altered metabolites (DAMs) analyses. In addition, exploration of the activities of 4‐coumarate‐CoA ligase (4CL), caffeoyl‐CoA O‐methyltransferase (CCoAOMT), peroxidase (POD), chalcone isomerase (CHI), dihydroflavonol‐4‐reductase (DFR), and flavonol synthase (FLS) further showed that phenylpropanoid and flavonoid pathways participate in plant responses to salt stress. Moreover, the phenylpropanoid pathways and flavonoid pathways were found to be potential pathways regulating plant response to salt stress based on transcriptome and metabolome analysis. The activities of 4CL, CCoAOMT, POD, CHI, DFR, and FLS revealed that these pathways are crucial to the regulation of plant responses to salt stress. These findings provided theoretical basis for further improvement of salt tolerance in common bean.

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The interplay between miR156/SPL13 and DFR/WD40–1 regulate drought tolerance in alfalfa

Cited by 108 publications

References 93 publications

Transcriptome-IPMS analysis reveals a tissue-dependent miR156/SPL13 regulatory mechanism in alfalfa drought tolerance

Transcriptome-IPMS analysis reveals a tissue-dependent miR156/SPL13 regulatory mechanism in alfalfa drought tolerance

Label-free quantitative proteomic analysis of alfalfa in response to microRNA156 under high temperature

Analysis of the transcriptome and metabolome reveals phenylpropanoid mechanism in common bean (Phaseolus vulgaris) responding to salt stress at sprout stage

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