The invasive Neisseria meningitidis MenC CC103 from Brazil is characterized by an accessory gene repertoire

Abanto, Michel; Fonseca, Érica L.; Encinas, Fernando; Freitas, Fernanda; Camargo, Dhian Almeida; Coimbra, Roney Santos; Filippis, Ivano de; Vicente, Ana Carolina Paulo

doi:10.1038/s41598-017-01671-x

Cited by 5 publications

(7 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nm-PG3 has NmSA22, NmSA44, NmSA15 (~40 kb), prophages predicted by Orazi et al (2022) [ 25 ], and IHT-E [ 9 ]. Nm-PG4 contains most of the prophages predicted in this study ( n = 21), which would be associated with MuMenB, including Pnm1, Pnm2, Phast1-FAM18, and Phast2-FAM18 mu-like prophages [ 12 , 15 ]. Nm-PG5 includes prophages ~41 kb in size and predicted by Orazi et al (2022) [ 25 ] and by us.…”

Section: Resultsmentioning

confidence: 99%

“…These prophages have been described in lineages belonging to specific serogroups, clonal complexes, or geographic regions. For instance, the MDA (Meningococcal Disease Associated) prophage copies (Nf1-C1, Nf1-C2, Nf1-C3, and Nf1-C4), IHT-E, and phast-2 have been identified in the FAM18 strain belonging to serogroup C and cc11 [ 9 , 10 , 11 , 12 ]. The presence of MDA in the genome of N. meningitidis , via the production of viral particles, increases the colonization of N. meningitidis in the nasopharynx.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comprehensive Genome Analysis of Neisseria meningitidis from South America Reveals a Distinctive Pathogenicity-Related Prophage Repertoire

Madariaga-Troncoso

Leyton-Carcaman

García

et al. 2022

IJMS

View full text Add to dashboard Cite

Neisseria meningitidis, a bacterium that colonizes in the human nasopharynx, occasionally causes invasive meningococcal disease leading to meningitis or septicemia. Different serogroups and lineages (clonal complexes) are related to the occurrence and epidemiology of N. meningitidis. Despite vaccines for most serogroups, N. meningitidis lineages causing unusual clinical manifestations and a higher fatality rate compared to other lineages have been reported in South America. The present study focused on exploring the diversity of N. meningitidis prophages from South America and their relationship with the epidemiological variables of these strains. We found a high diversity of prophages among the different clonal complexes. By comparing them with previously described N. meningitidis phages and prophages, we revealed groups of prophages sharing similar compositions, which could be useful for prophage comparison in N. meningitidis. Furthermore, we observed a high correlation between the prophage content and epidemiological features, e.g., pathogenicity or clonal complex. Additionally, a distinctive filamentous prophage named here as IMSAR-11 (Invasive Meningococci from South America Related to cc11) was identified. Interestingly, two versions of IMSAR-11, circular and chromosomally integrated, were found. Overall, this study reinforces the importance of the genomic characterization of circulating N. meningitidis lineages to generate new targets for lineage monitoring, diagnosis, or appropriateness of vaccine development. Further studies are necessary to understand the role of these prophages in the persistence, dispersal, and virulence of N. meningitidis in the world.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comprehensive Genome Analysis of Neisseria meningitidis from South America Reveals a Distinctive Pathogenicity-Related Prophage Repertoire

Madariaga-Troncoso

Leyton-Carcaman

García

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…To the best of our knowledge, the only previously characterized neisserial ICE is a GGI-like meningococcal element, ICE Nm CC103, associated with an invasive clonal complex and suggested to contribute to persistence of this lineage by aiding meningococcal defences against oxidative stress-induced DNA damage [ 75 ]. blastn comparisons between ICE Nm CC103 and all ICE riNK sequences listed in Fig.…”

Section: Resultsmentioning

confidence: 99%

Identification of integrative and conjugative elements in pathogenic and commensal Neisseriaceae species via genomic distributions of DNA uptake sequence dialects

et al. 2020

View full text Add to dashboard Cite

Mobile genetic elements (MGEs) are key factors responsible for dissemination of virulence determinants and antimicrobial-resistance genes amongst pathogenic bacteria. Conjugative MGEs are notable for their high gene loads donated per transfer event, broad host ranges and phylogenetic ubiquity amongst prokaryotes, with the subclass of chromosomally inserted integrative and conjugative elements (ICEs) being particularly abundant. The focus on a small number of model systems has biased the study of ICEs towards those conferring readily selectable phenotypes to host cells, whereas the identification and characterization of integrated cryptic elements remains challenging. Even though antimicrobial resistance and horizontally acquired virulence genes are major factors aggravating neisserial infection, conjugative MGEs of Neisseria gonorrhoeae and Neisseria meningitidis remain poorly characterized. Using a phenotype-independent approach based on atypical distributions of DNA uptake sequences (DUSs) in MGEs relative to the chromosomal background, we have identified two groups of chromosomally integrated conjugative elements in Neisseria : one found almost exclusively in pathogenic species possibly deriving from the genus Kingella , the other belonging to a group of Neisseria mucosa -like commensals. The former element appears to enable transfer of traditionally gonococcal-specific loci such as the virulence-associated toxin–antitoxin system fitAB to N. meningitidis chromosomes, whilst the circular form of the latter possesses a unique attachment site ( attP ) sequence seemingly adapted to exploit DUS motifs as chromosomal integration sites. In addition to validating the use of DUS distributions in Neisseriaceae MGE identification, the >170 identified ICE sequences provide a valuable resource for future studies of ICE evolution and host adaptation.

show abstract

“…Based on bacterial genome sequences, 24 invasive strains of N. meningitidis serogroup C from Brazil were mapped, and enzyme-encoding gene studies for the synthesis of meningococcal polysaccharide capsule showed that these genes are located on pathogenicity islands and organized as an operon (66). Furthermore, the whole genome sequencing (WGS) method leads to advances in the detection of isolated N. meningitidis strains (67).…”

Section: Capsular Polysaccharides and Serotypingmentioning

confidence: 99%

Characteristics of Neisseria Species Colonized in the Human’s Nasopharynx

Tavana

Ataee

Jonaidi

et al. 2020

Jundishapur J Microbiol

View full text Add to dashboard Cite

Context: Neisseria meningitidis is the causative agent of a life-threatening infection with high mortality and morbidity worldwide. The most common types of this bacterium are serogroups A, B, C, W135, X, and Y. Although in some countries, such as Iran, the meningococcal meningitis has been well monitored and controlled by the use of divalent and quadrivalent vaccines, other fatal infections caused by these bacteria are still an important threat. For the above reason, this review focused on the differences of Neisseria characteristics, particularly in capsular composition, pathogenic and commensal stages to a better understanding of how to manage Neisseria infections. Evidence Acquisition: In this review, PubMed, EMBASE, ScienceDirect, Scopus, and Google Scholar were searched for English-language publications on pathogenic or commensal strains of Neisseria, meningococcal disease, Neisseria biology, genetic diversity, molecular typing, serogroups, diagnostic, and epidemiology around the world up to July 2019. All articles and academic reports in the defined area of this research were considered too. The data were extracted and descriptively discussed. Results: We included 85 studies in the survey. The data analysis revealed that the distribution of meningococcal serogroups was different regionally. For example, the serogroups C and W-135 accounted for Africa and Latin America regions, serogroup B in the European countries, and rarely in the Western Pacific, and serogroups A and C were dominant in Asian countries. Although data set for laboratory-based diagnosis of N. meningitidis are available for all countries, only 30% of the countries rely on reference laboratories for serogroup determination, and more than half of the countries lack the ability of surveillance system. Nevertheless, molecular detection procedure is also available for all countries. The use of the meningococcal vaccine is a variable country by country, but most countries have applied the meningococcal vaccine, either divalent or quadrivalent, for the protection of high-risk groups. Conclusions: Owing to the geographical distribution of N. meningitidis serogroups in circulating, each country has to monitor for changes in serogroups diversity and its control management. Furthermore, laboratories should scale up the epidemiology and disease burden. It should be mentioned that quadrivalent meningococcal vaccines reduce the meningococcal disease burden sharply.

show abstract

The invasive Neisseria meningitidis MenC CC103 from Brazil is characterized by an accessory gene repertoire

Cited by 5 publications

References 58 publications

Comprehensive Genome Analysis of Neisseria meningitidis from South America Reveals a Distinctive Pathogenicity-Related Prophage Repertoire

Comprehensive Genome Analysis of Neisseria meningitidis from South America Reveals a Distinctive Pathogenicity-Related Prophage Repertoire

Identification of integrative and conjugative elements in pathogenic and commensal Neisseriaceae species via genomic distributions of DNA uptake sequence dialects

Characteristics of Neisseria Species Colonized in the Human’s Nasopharynx

Contact Info

Product

Resources

About