The isolation of specific transfer ribonucleic acids

The preliminary sequence of tRNAMetF from Anacystis nidulans compared with other initiator tRNAs

“…The [32p]methionine tRNAs were isolated using the procedure of Gillam et al [11] and separated by RPC-5 column chromatography [12]. Aminoacylation of algal methionine tRNA was performed by the E. coli aminoacyl-tRNA synthetase [ 12].…”

Initiation of protein synthesis with mischarged tRNAMetf fromE. coli

“…The chemical formylation procedure [6,7] of aminoacyl-tRNA's has been used instead of the enzymatic one [3] because the former not only gives better yields but also makes possible the formylation of all aminoacyl-tRNA's tested. The formylation obtained ranged from 75 to 90% as judged from samples subjected to alkaline hydrolysis by NH4OH followed by paper electrophoresis at pH 3.5.…”

Specific spin-labeling of transfer ribonucleic acid molecules

“…We then conclude from this that the 3'end terminal adenosine was indeed transformed into its morpholino derivatives MSL. Table 1 summarizes the results and gives the location of labeling and the nature of the attachment of the labels as based on previous results (4)(5)(6)10,16). For all the elution patterns, only one peak contains the paramagnetic material, the analysis of which showing that in all cases only one position on the tRNA molecule carries the label, and this in spite of the fact that other groups are also succeptible to react.…”