The key genes and pathways related to male sterility of eggplant revealed by comparative transcriptome analysis

Yang, Yan; Bao, Shengyou; Zhou, Xiaohui; Li, Jun; Zhuang, Yun

doi:10.1186/s12870-018-1430-2

Cited by 39 publications

(28 citation statements)

References 65 publications

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“…NADH dehydrogenase (ubiquinone), oxidoreductase, and photosynthetic electron transport play a crucial role in energy production and conversion, in which the proteins are components in the mitochondrial respiratory chain. This showed that mitochondrial respiratory-related enzymes play an important role in the regulation of the CMS line C2P5A, and this result is consistent with a previous study in CMS plants [85][86][87]. Pectinesterase is a wall-degrading enzyme, and abnormal expression may lead to the disruption of the cell wall structure in the CMS line C2P5A; a previous study demonstrated that pectinesterase plays a major role in the plant [88].…”

Section: Lncrna In the Anther Development Of Cotton And Predicted Funsupporting

confidence: 91%

“…Significant KEGG pathways were primarily enriched for several processes, such as fatty acid biosynthesis, flavonoid biosynthesis, glutathione metabolism, MAPK signaling pathway to the plant, oxidative phosphorylation, ubiquinone and other terpenoid quinone biosynthesis, pentose and glucuronate interconversions, and plant hormone signal transduction ( Figure 6A-C). Previous studies show that these metabolism pathways play a vital role during anther development in flowering plants, in which a disturbed metabolism pathway seriously leads to the impairment of anther development, and causes male sterility [55,57,61,62,85,89]. This study showed that some lncRNAs and mRNAs might play important roles in anther development, such as cis-target gene Ghir_A09G011050.1 of LTCONS_00105434 through GO:0008137 (NADH dehydrogenase (ubiquinone) activity) and ko00190 (oxidative phosphorylation); cis-target gene Ghir_A01G005150.1 of LTCONS_00004262 through GO:0016491 (oxidoreductase activity) and ko00941 (flavonoid biosynthesis); cis-target gene Ghir_D05G003710.2 of LTCONS_00126105 through GO:0005975 (carbohydrate metabolic process) and ko00040 (pentose and glucuronate interconversions); cis-target gene Ghir_A03G016640.1 of LTCONS_00085561 through GO:0016790 (catalytic activity) and ko00061 (fatty acid biosynthesis); and cis-target gene Ghir_A12G005100.1 of LTCONS_00085561 through GO:0005524 (ATP binding) and ko04016 (MAPK signaling pathway to the plant).…”

Section: Lncrna In the Anther Development Of Cotton And Predicted Funmentioning

confidence: 99%

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Integrative Analysis of the lncRNA and mRNA Transcriptome Revealed Genes and Pathways Potentially Involved in the Anther Abortion of Cotton (Gossypium hirsutum L.)

Qin

Dong

et al. 2019

Genes

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Cotton plays an important role in the economy of many countries. Many studies have revealed that numerous genes and various metabolic pathways are involved in anther development. In this research, we studied the differently expressed mRNA and lncRNA during the anther development of cotton between the cytoplasmic male sterility (CMS) line, C2P5A, and the maintainer line, C2P5B, using RNA-seq analysis. We identified 17,897 known differentially expressed (DE) mRNAs, and 865 DE long noncoding RNAs (lncRNAs) that corresponded to 1172 cis-target genes at three stages of anther development using gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment of DE mRNAs; and cis-target genes of DE lncRNAs probably involved in the degradation of tapetum cells, microspore development, pollen development, and in the differentiation, proliferation, and apoptosis of the anther cell wall in cotton. Of these DE genes, LTCONS_00105434, LTCONS_00004262, LTCONS_00126105, LTCONS_00085561, and LTCONS_00085561, correspond to cis-target genes Ghir_A09G011050.1, Ghir_A01G005150.1, Ghir_D05G003710.2, Ghir_A03G016640.1, and Ghir_A12G005100.1, respectively. They participate in oxidative phosphorylation, flavonoid biosynthesis, pentose and glucuronate interconversions, fatty acid biosynthesis, and MAPK signaling pathway in plants, respectively. In summary, the transcriptomic data indicated that DE lncRNAs and DE mRNAs were related to the anther development of cotton at the pollen mother cell stage, tetrad stage, and microspore stage, and abnormal expression could lead to anther abortion, resulting in male sterility of cotton.

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Section: Lncrna In the Anther Development Of Cotton And Predicted Funsupporting

confidence: 91%

Section: Lncrna In the Anther Development Of Cotton And Predicted Funmentioning

confidence: 99%

Integrative Analysis of the lncRNA and mRNA Transcriptome Revealed Genes and Pathways Potentially Involved in the Anther Abortion of Cotton (Gossypium hirsutum L.)

Qin

Dong

et al. 2019

Genes

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“…With the advancement in genomics and transcriptomics studies, the effort has been made ease in identifying molecular marker linked to the Rf-genes which promote the integration of Rf-genes in various eggplant genetic backgrounds. Recently, comparative transcriptome analysis has been performed to identify the MS-related genes and pathway in the eggplant of the male sterile line and its maintainer line [103]. The finding of this study provided insight into key genes and pathways associated with eggplant male sterility, which provided a primary basis for further research on fertility and anther development.…”

Section: Prospects Of Male Sterility In Eggplant Heterosismentioning

confidence: 85%

Heterosis Breeding in Eggplant (Solanum melongena L.): Gains and Provocations

Kumar

Sharma

Jain

et al. 2020

Plants

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Heterosis (or hybrid vigor) results in a hybrid’s phenotypic superiority over its founder parents for quantitative and qualitative traits. Hybrid vigor is defined by mechanisms such as dominant complementation, over-dominance, and epistasis. Eggplant (Solanum melongena L.) is an essential vegetable crop and a good source of dietary minerals, vitamins, and anthocyanins, with a high oxygen radical absorbance capacity and low caloric value. Given the economic and nutritional significance of eggplants, breeding efforts focus on developing high-yielding varieties—mostly F1 hybrids—with important traits. Studies indicate the successful exploitation of heterosis in the eggplant for a considerable improvement with respect to quantitative traits. In this direction, estimating heterosis for yield-related traits could well be useful for examining the most beneficial hybrid mix with the exploitation of top-quality hybrid. This review examines the current perception of the breeding and molecular aspects of heterosis in eggplants and cites several studies describing the mechanisms. Rendering and combining recent genomics, epigenetic, proteomic, and metabolomics studies present new prospects towards the understanding of the regulatory events of heterosis involved in the evolution and the domestication of the eggplant ideotype.

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“…Understanding the molecular mechanisms controlling anther development and male sterility is of great importance for heterosis utilization in many crop species, including cotton. In recent years, male sterility lines have been widely studied by transcriptomic analysis in different species, such as wheat [56,57], maize [58], eggplant [59] and cotton [60][61][62][63]. However, most studies tend to carry out extensive enrichment of DEGs without systematic classi cation analysis based on different development stages.…”

Section: Discussionmentioning

confidence: 99%

Deficiencies in the formation and regulation of anther cuticle and tryphine contribute to male sterility in cotton PGMS line

Zhang

Liu

et al. 2020

Preprint

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Background: Male sterility is a simple and efficient pollination control system that is widely exploited in hybrid breeding. In upland cotton, CCRI9106, a photosensitive genetic male sterile (PGMS) mutant isolated from CCRI040029, was reported of great advantages to cotton heterosis. However, little information concerning the male sterility of CCRI9106 is known. Here, comparative transcriptome analysis of CCRI9106 (the mutant, MT) and CCRI040029 (the wild type, WT) anthers in Anyang (long-day, male sterile condition to CCRI9106) was performed to reveal the potential male sterile mechanism of CCRI9106.Results: Light and electron microscopy revealed that the male sterility phenotype of MT was mainly attributed to irregularly exine, lacking tryphine and immature anther cuticle. Based on the cytological characteristics of MT anthers, anther RNA libraries (18 in total) of tetrad (TTP), late uninucleate (LUNP) and binucleate (BNP) stages in MT and WT were constructed for transcriptomic analysis, therefore revealing a total of 870,4 differentially expressed genes (DEGs). By performing gene expression pattern analysis and protein-protein interaction (PPI) networks construction, we found down-regulation of DEGs, which enriched by the lipid biosynthetic process and the synthesis pathways of several types of secondary metabolites such as terpenoids, flavonoids and steroids, may crucial to the male sterility phenotype of MT, and resulting in the defects of anther cuticle and tryphine, even the irregularly exine. Furthermore, several lipid-related genes together with ABA-related genes and MYB transcription factors were identified as hub genes via weighted gene co-expression network analysis (WGCNA). Additionally, the ABA content of MT anthers was reduced across all stages when compared with WT anthers. At last, genes related to the formation of anther cuticle and tryphine could activated in MT under short-day condition.Conclusions: We propose that the down-regulation of genes related to the assembly of anther cuticle and tryphine may lead to the male sterile phenotype of MT, and MYB transcription factors together with ABA played key regulatory roles in these processes. The conversion of fertility in different photoperiods may closely relate to the functional expression of these genes. These findings contribute to elucidate the mechanism of male sterility in upland cotton.

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The key genes and pathways related to male sterility of eggplant revealed by comparative transcriptome analysis

Cited by 39 publications

References 65 publications

Integrative Analysis of the lncRNA and mRNA Transcriptome Revealed Genes and Pathways Potentially Involved in the Anther Abortion of Cotton (Gossypium hirsutum L.)

Integrative Analysis of the lncRNA and mRNA Transcriptome Revealed Genes and Pathways Potentially Involved in the Anther Abortion of Cotton (Gossypium hirsutum L.)

Heterosis Breeding in Eggplant (Solanum melongena L.): Gains and Provocations

Deficiencies in the formation and regulation of anther cuticle and tryphine contribute to male sterility in cotton PGMS line

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