The Kinase ERULUS Controls Pollen Tube Targeting and Growth in Arabidopsis thaliana

Schoenaers, Sébastjen; Balcerowicz, Daria; Costa, Alex; Vissenberg, Kris

doi:10.3389/fpls.2017.01942

Cited by 33 publications

(31 citation statements)

References 56 publications

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“…Previous studies of CrRLK1L proteins where mutation results in low or absent seed set have identified functions in pollen tube growth (ANX1, ANX2, BUPS1, BUPS2 and ERU [11][12][13][14][15]) and femalemediated pollen tube growth arrest at the synergids (FER [17]). To test which step in fertilisation is impaired in the herk1 anj mutant, we tracked pollen tube growth through the style and ovary in single and double mutants.…”

Section: Herk1 and Anj Are Female Determinants Of Pollen Tube Receptionmentioning

confidence: 99%

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CrRLK1L receptor‐like kinases HERK1 and ANJEA are female determinants of pollen tube reception

et al. 2019

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Communication between the gametophytes is vital for angiosperm fertilisation. Multiple CrRLK1L-type receptor kinases prevent premature pollen tube burst, while another CrRLK1L protein, FERONIA (FER), is required for pollen tube reception in the female gametophyte. We report here the identification of two additional CrRLK1L homologues, HERCULES RECEPTOR KINASE 1 (HERK1) and ANJEA (ANJ), which act redundantly to promote pollen tube growth arrest at the synergid cells. HERK1 and ANJ localise to the filiform apparatus of the synergid cells in unfertilised ovules, and in herk1 anj mutants, a majority of ovules remain unfertilised due to pollen tube overgrowth, together indicating that HERK1 and ANJ act as female determinants for fertilisation. As in fer mutants, the synergid cell-specific, endomembrane protein NORTIA (NTA) is not relocalised after pollen tube reception; however, unlike fer mutants, reactive oxygen species levels are unaffected in herk1 anj double mutants. Both ANJ and HERK1 associate with FER and its proposed co-receptor LORELEI (LRE) in planta. Together, our data indicate that HERK1 and ANJ act with FER to mediate female-male gametophyte interactions during plant fertilisation.

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Section: Herk1 and Anj Are Female Determinants Of Pollen Tube Receptionmentioning

confidence: 99%

“…[10] for a detailed review). A fifth CrRLK1L protein, ERULUS (ERU), has also been implicated in male-determined pollen tube growth via regulation of Ca 2+ oscillations [15]. A fifth CrRLK1L protein, ERULUS (ERU), has also been implicated in male-determined pollen tube growth via regulation of Ca 2+ oscillations [15].…”

Section: Introductionmentioning

confidence: 99%

CrRLK1L receptor‐like kinases HERK1 and ANJEA are female determinants of pollen tube reception

et al. 2019

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“…To provide further evidence that ROP signaling is compromised in agcdm pollen tubes, we examined a few intracellular activities that are downstream of ROP signaling (Fu et al, 2001;Hwang et al, 2005;Lavy et al, 2007). We first introduced yellow cameleon 3.6 (YC3.6), a ratio-imaging Ca 2+ probe (Iwano et al, 2009;Schoenaers et al, 2017), to examine Ca 2+ levels in the pollen tubes. As reported previously (Iwano et al, 2009;Schoenaers et al, 2017), wild-type pollen tubes contained a tip-focused Ca 2+ gradient with maximal concentrations at the apical region (Figure 2A).…”

Section: Loss Of Agc15 and Agc17 Functions Compromised Rop Downstrementioning

confidence: 99%

AGC1.5 Kinase Phosphorylates RopGEFs to Control Pollen Tube Growth

Cui

et al. 2018

Molecular Plant

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Double fertilization in angiosperms requires the targeted delivery of immotile sperm to the eggs through pollen tubes. The polarity of tip-growing pollen tubes is maintained through dynamic association of active Rho GTPases of plants (ROP-GTP) with the apical plasma membrane. Guanine nucleotide exchange factors for ROPs (RopGEFs) catalyze the activation of ROPs and thereby affect spatiotemporal ROP signaling. Whereas RopGEFs have been found to be phosphorylated proteins, the kinases responsible for their phosphorylation in vivo and biological consequences of RopGEF phosphorylation in pollen tube growth remain unclear. We report here that the Arabidopsis AGC1.5 subfamily of cytoplasmic kinases is critical for the restricted localization of ROP-GTP during pollen tube growth. Loss of AGC1.5 and AGC1.7 functions resulted in the mistargeting of active ROPs and defective events downstream of ROP signaling in pollen tubes. AGC1.5 interacts with RopGEFs via their catalytic PRONE domain and phosphorylates RopGEFs at a conserved Ser residue of PRONE domain. Loss of AGC1.5 and AGC1.7 functions resulted in the mistargeting of RopGEFs in pollen tubes, similar to the phenotype caused by the mutation that renders RopGEFs non-phosphorylatable by AGC1.5. Collectively, our results provide mechanistic insights into the spatiotemporal activation of ROPs during the polar growth of pollen tubes.

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“…Most of these belong to the family of malectin‐like receptor kinases (RLKs), also known as the Catharanthus roseus RLK‐like proteins ( Cr RLK1Ls), which in Arabidopsis comprises 17 members (Franck et al ., ). Within this family, ERULUS (ERU) is an important component of the fertilization pathway and regulates pollen tube targeting in vivo (Schoenaers et al ., ). ERU also regulates the dynamics of pectin methylation/de‐methylation during the growth of the root hair tip by functionally interacting with another CrRLK1L member, FERONIA (FER) (Schoenaers et al ., ; Kwon et al ., ).…”

Section: Developmentmentioning

confidence: 97%

“…Within this family, ERULUS (ERU) is an important component of the fertilization pathway and regulates pollen tube targeting in vivo (Schoenaers et al ., ). ERU also regulates the dynamics of pectin methylation/de‐methylation during the growth of the root hair tip by functionally interacting with another CrRLK1L member, FERONIA (FER) (Schoenaers et al ., ; Kwon et al ., ). FER is required for growth of root hairs as well as of trichomes, which both lose their integrity upon or shortly after emergence in the loss‐of‐function fer mutants (Franck et al ., ).…”

Section: Developmentmentioning

confidence: 97%

Cell wall traits that influence plant development, immunity, and bioconversion

et al. 2019

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Summary The architecture of the plant cell wall is highly dynamic, being substantially re‐modeled during growth and development. Cell walls determine the size and shape of cells and contribute to the functional specialization of tissues and organs. Beyond the physiological dynamics, the wall structure undergoes changes upon biotic or abiotic stresses. In this review several cell wall traits, mainly related to pectin, one of the major matrix components, will be discussed in relation to plant development, immunity and industrial bioconversion of biomass, especially for energy production. Plant cell walls are a source of oligosaccharide fragments with a signaling function for both development and immunity. Sensing cell wall damage, sometimes through the perception of released damage‐associated molecular patterns (DAMPs), is crucial for some developmental and immunity responses. Methodological advances that are expected to deepen our knowledge of cell wall (CW) biology will also be presented.

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The Kinase ERULUS Controls Pollen Tube Targeting and Growth in Arabidopsis thaliana

Cited by 33 publications

References 56 publications

CrRLK1L receptor‐like kinases HERK1 and ANJEA are female determinants of pollen tube reception

CrRLK1L receptor‐like kinases HERK1 and ANJEA are female determinants of pollen tube reception

AGC1.5 Kinase Phosphorylates RopGEFs to Control Pollen Tube Growth

Cell wall traits that influence plant development, immunity, and bioconversion

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